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学科主题: 临床医学
题名:
A resonance light scattering sensor based on methylene blue-sodium dodecyl benzene sulfonate for ultrasensitive detection of guanine base associated mutations
作者: Chen, Zhanguang1; Qian, Sihua1; Chen, Junhui2; Chen, Xi3; Zheng, Liwen1; Liu, Jinbin4
关键词: Resonance light scattering ; Methylene blue ; Sodium dodecyl benzene sulfonate ; Guanine bases ; Mutations
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2012-10-01
DOI: 10.1007/s00216-012-6289-8
卷: 404, 期:6-7, 页:1673-1679
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: FACTOR-V-LEIDEN ; FLOW-INJECTION ANALYSIS ; NUCLEIC-ACIDS ; RAYLEIGH-SCATTERING ; IN-VITRO ; DNA ; PCR ; OLIGONUCLEOTIDES ; ELECTROPHORESIS ; AGGREGATION
英文摘要:

A resonance light scattering (RLS) sensor for guanine base associated mutations has been developed on the basis of the high selectivity of methylene blue (MB) for guanine bases in the presence of sodium dodecyl benzene sulfonate (SDBS). MB, when bound to SDBS, underwent a dramatic enhancement of its RLS intensity. However, the addition of double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) caused the strong RLS intensity of MB-SDBS to decrease, and the RLS intensity of MB-SDBS-ssDNA was much lower than that of MB-SDBS-dsDNA. Consequently, it can be concluded that the binding abilities of MB-SDBS with ssDNA and dsDNA were different. Besides, the experimental results showed that MB-SDBS could bind specifically to oligonucleotides rich in guanine bases. Short DNA targets with sequences related to beta-thalassaemia, thrombophilia and psoriasis, all of which are guanine base relevant mutations, were synthesized. It was found that MB-SDBS could recognize the single-base mismatches in the mutational DNA, followed by different RLS signal changes between MB-SDBS-normal DNA systems and MB-SDBS-mutational DNA systems. The ultrasensitive sensor allows simple, rapid, sensitive and selective detection of guanine base associated mutations, indicating its potential application in the medical field.

语种: 英语
所属项目编号: 2006B35630009 ; YR09009
项目资助者: Guangdong Science and Technology Department ; Science Foundation of Shantou University
WOS记录号: WOS:000309348400007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63620
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Univ Texas Dallas, Dept Chem, Richardson, TX 75080 USA
3.Peking Univ, Shenzhen Hosp, Intervent Oncol & Minimally Invas Therapies Dept, Shenzhen 518036, Peoples R China
4.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China

Recommended Citation:
Chen, Zhanguang,Qian, Sihua,Chen, Junhui,et al. A resonance light scattering sensor based on methylene blue-sodium dodecyl benzene sulfonate for ultrasensitive detection of guanine base associated mutations[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2012,404(6-7):1673-1679.
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