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学科主题: 基础医学
题名:
Effects of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion
作者: Chen, Wei-Xing2,3; Wang, Fang3; Liu, Yu-Ying3; Zeng, Qing-Jiang3; Sun, Kai3; Xue, Xin3; Li, Xiang3; Yang, Ji Ying2,3; An, Li-Hua3; Hu, Bai-He3; Yang, Jin-Hui3; Wang, Chuan-She1,3; Li, Zhi-Xin1,3; Liu, Lian-Yi3; Li, Yan3; Zheng, Jun2,3; Liao, Fu-Long3; Han, Dong3; Fan, Jing-Yu3; Han, Jing-Yan1,3
关键词: ischemia/reperfusion ; notoginsenoside R1 ; leukocytes adhesion ; E-selectin ; hepatic injury
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2008-01-07
DOI: 10.3748/wjg.14.29
卷: 14, 期:1, 页:29-37
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: NF-KAPPA-B ; INTERCELLULAR-ADHESION MOLECULE-1 ; INDUCED LIVER-INJURY ; MICROVASCULAR DYSFUNCTION ; PANAX-NOTOGINSENG ; IN-VITRO ; TRANSENDOTHELIAL MIGRATION ; ENDOTHELIAL-CELLS ; HERBAL MEDICINE ; FED ETHANOL
英文摘要:

AIM: To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion (I/R) in mice.

METHODS: The superior mesenteric artery (SMA) of C57/BL mice was ligated for 15 min to induce gut ischemia followed by 30-min reperfusion. In another set of experiments, R1 was continuously infused (10 mg/kg per hour) from 10 min before I/R until the end of the investigation to study the influence of R1 on hepatic microcirculatory disturbance induced by gut I/R. Hepatic microcirculation was observed by inverted microscopy, and the vascular diameter, red blood cell (RBC) velocity and sinusoid perfusion were estimated. Leukocyte rolling and adhesion were observed under a laser confocal microscope. Thirty and 60 min after reperfusion, lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate transaminase (AST) in peripheral blood were determined. The expression of adhesion molecules CD11b/CD18 in neutrophils and tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in plasma were evaluated by flow cytometry. E-selectin and intercellular adhesion molecule-1 (ICAM-1) in hepatic tissue were examined by immunofluorescence.

RESULTS: After gut I/R, the diameters of terminal portal venules and central veins, RBC velocity and the number of perfused sinusoids were decreased, while the leukocyte rolling and adhesion, the expression of E-selectin in hepatic vessels and CD18 in neutrophils, IL-6, MCP-1, LDH, ALT and AST were increased. R1 treatment attenuated these alterations except for IL-6 and MCP-1.

CONCLUSION: R1 prevents I/R-induced hepatic microcirculation disturbance and hepatocyte injury. The effect of R1 is related to its inhibition of leukocyte rolling and adhesion by inhibiting the expression of E-selectin in endothelium and CD18 in neutrophils.

语种: 英语
WOS记录号: WOS:000252240000004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63654
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Integrated Chines & Western Med, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Tianjin Univ Tradit Chinese Med, Dept Pharmacol, Tianjin 300193, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Chen, Wei-Xing,Wang, Fang,Liu, Yu-Ying,et al. Effects of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(1):29-37.
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