北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病原生物学系  > 期刊论文
学科主题: 基础医学
题名:
Thermal stability and inactivation of hepatitis C virus grown in cell culture
作者: Song, Hongshuo1; Li, Jin1; Shi, Shuang1; Yan, Ling1; Zhuang, Hui1; Li, Kui2
刊名: VIROLOGY JOURNAL
发表日期: 2010-02-18
DOI: 10.1186/1743-422X-7-40
卷: 7, 期:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: PLASMA PRODUCTS ; INFECTION ; TEMPERATURE ; CONCENTRATE ; RELEVANT
英文摘要:

Background: Hepatitis C virus (HCV) is a blood-borne flavivirus that infects many millions of people worldwide. Relatively little is known, however, concerning the stability of HCV and reliable procedures for inactivating this virus.

Methods: In the current study, the thermostability of cell culture-derived HCV (HCVcc, JFH-1 strain) under different environmental temperatures (37 degrees C, room temperature, and 4 degrees C) and the ability of heat, UVC light irradiation, and aldehyde and detergent treatments to inactivate HCVcc were evaluated. The infectious titers of treated viral samples were determined by focus-forming unit (FFU) assay using an indirect immunofluorescence assay for HCV NS3 in hepatoma Huh7-25-CD81 cells highly permissive for HCVcc infection. MTT cytotoxicity assay was performed to determine the concentrations of aldehydes or detergents at which they were no longer cytotoxic.

Results: HCVcc in culture medium was found to survive 37 degrees C and room temperature (RT, 25 +/- 2 degrees C) for 2 and 16 days, respectively, while the virus was relatively stable at 4 degrees C without drastic loss of infectivity for at least 6 weeks. HCVcc in culture medium was sensitive to heat and could be inactivated in 8 and 4 min when incubated at 60 degrees C and 65 degrees C, respectively. However, at 56 degrees C, 40 min were required to eliminate HCVcc infectivity. Addition of normal human serum to HCVcc did not significantly alter viral stability at RT or its susceptibility to heat. UVC light irradiation (wavelength = 253.7 nm) with an intensity of 450 mu W/cm(2) efficiently inactivated HCVcc within 2 min. Exposures to formaldehyde, glutaraldehyde, ionic or nonionic detergents all destroyed HCVcc infectivity effectively, regardless of whether the treatments were conducted in the presence of cell culture medium or human serum.

Conclusions: The results provide quantitative evidence for the potential use of a variety of approaches for inactivating HCV. The ability of HCVcc to survive ambient temperatures warrants precautions in handling and disposing of objects and materials that may have been contaminated with HCV.

语种: 英语
所属项目编号: 2008ZX10002-013 ; R01-AI069285
项目资助者: China Eleventh Five-year Plan ; National Key Science ; National Institute of Allergy and Infectious Diseases
WOS记录号: WOS:000275509500001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63655
Appears in Collections:基础医学院_病原生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Thermal stability and inactivation of hepatitis C virus grown in cell culture.pdf(436KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
2.Univ Tennessee, Hlth Sci Ctr, Dept Mol Sci, Memphis, TN 38163 USA

Recommended Citation:
Song, Hongshuo,Li, Jin,Shi, Shuang,et al. Thermal stability and inactivation of hepatitis C virus grown in cell culture[J]. VIROLOGY JOURNAL,2010,7(1).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Song, Hongshuo]'s Articles
[Li, Jin]'s Articles
[Shi, Shuang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Song, Hongshuo]‘s Articles
[Li, Jin]‘s Articles
[Shi, Shuang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace