IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Thermal stability and inactivation of hepatitis C virus grown in cell culture
Song, Hongshuo1; Li, Jin1; Shi, Shuang1; Yan, Ling1; Zhuang, Hui1; Li, Kui2
刊名VIROLOGY JOURNAL
2010-02-18
DOI10.1186/1743-422X-7-40
7期:1
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]PLASMA PRODUCTS ; INFECTION ; TEMPERATURE ; CONCENTRATE ; RELEVANT
英文摘要

Background: Hepatitis C virus (HCV) is a blood-borne flavivirus that infects many millions of people worldwide. Relatively little is known, however, concerning the stability of HCV and reliable procedures for inactivating this virus.

Methods: In the current study, the thermostability of cell culture-derived HCV (HCVcc, JFH-1 strain) under different environmental temperatures (37 degrees C, room temperature, and 4 degrees C) and the ability of heat, UVC light irradiation, and aldehyde and detergent treatments to inactivate HCVcc were evaluated. The infectious titers of treated viral samples were determined by focus-forming unit (FFU) assay using an indirect immunofluorescence assay for HCV NS3 in hepatoma Huh7-25-CD81 cells highly permissive for HCVcc infection. MTT cytotoxicity assay was performed to determine the concentrations of aldehydes or detergents at which they were no longer cytotoxic.

Results: HCVcc in culture medium was found to survive 37 degrees C and room temperature (RT, 25 +/- 2 degrees C) for 2 and 16 days, respectively, while the virus was relatively stable at 4 degrees C without drastic loss of infectivity for at least 6 weeks. HCVcc in culture medium was sensitive to heat and could be inactivated in 8 and 4 min when incubated at 60 degrees C and 65 degrees C, respectively. However, at 56 degrees C, 40 min were required to eliminate HCVcc infectivity. Addition of normal human serum to HCVcc did not significantly alter viral stability at RT or its susceptibility to heat. UVC light irradiation (wavelength = 253.7 nm) with an intensity of 450 mu W/cm(2) efficiently inactivated HCVcc within 2 min. Exposures to formaldehyde, glutaraldehyde, ionic or nonionic detergents all destroyed HCVcc infectivity effectively, regardless of whether the treatments were conducted in the presence of cell culture medium or human serum.

Conclusions: The results provide quantitative evidence for the potential use of a variety of approaches for inactivating HCV. The ability of HCVcc to survive ambient temperatures warrants precautions in handling and disposing of objects and materials that may have been contaminated with HCV.

语种英语
WOS记录号WOS:000275509500001
项目编号2008ZX10002-013 ; R01-AI069285
资助机构China Eleventh Five-year Plan ; National Key Science ; National Institute of Allergy and Infectious Diseases
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63655
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
2.Univ Tennessee, Hlth Sci Ctr, Dept Mol Sci, Memphis, TN 38163 USA
推荐引用方式
GB/T 7714
Song, Hongshuo,Li, Jin,Shi, Shuang,et al. Thermal stability and inactivation of hepatitis C virus grown in cell culture[J]. VIROLOGY JOURNAL,2010,7(1).
APA Song, Hongshuo,Li, Jin,Shi, Shuang,Yan, Ling,Zhuang, Hui,&Li, Kui.(2010).Thermal stability and inactivation of hepatitis C virus grown in cell culture.VIROLOGY JOURNAL,7(1).
MLA Song, Hongshuo,et al."Thermal stability and inactivation of hepatitis C virus grown in cell culture".VIROLOGY JOURNAL 7.1(2010).
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