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学科主题基础医学
Characterization of the in vivo dynamics of medullary CD4(+)CD8(-) thymocyte development
Jin, Rong; Wang, Wei; Yao, Jin-Yan; Zhou, Yu-Bin; Qian, Xiao-Ping; Zhang, Jun; Zhang, Yu; Chen, Wei-Feng
刊名JOURNAL OF IMMUNOLOGY
2008-02-15
180期:4页:2256-2263
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]T-CELL DEVELOPMENT ; LYMPHOKINE SECRETION ; NEGATIVE SELECTION ; THYMIC EMIGRATION ; MOUSE THYMUS ; MATURATION ; RECEPTOR ; IDENTIFICATION ; PROLIFERATION ; EXPRESSION
英文摘要

Our previous studies have defined a differentiation program followed by the newly generated single-positive (SP) thymocytes before their emigration to the periphery. In the present study, we further characterize the development of CD4SP cells in the thymic medulla using mainly intrathymic adoptive transfer assays. By analyzing the differentiation kinetics of the donor cells, which were shown to home correctly to the medullary region following adoptive transfer, we established the precursor-progeny relationship among the four subsets of CD4SP thymocytes (SP1-SP4) and demonstrated that the progression from SP1 to SP4 was unidirectional and largely synchronized. Notably, while the phenotypic maturation from SP1 to SP4 was achieved in 2-3 days, a small fraction of donor cells could be retained in the thymus for a longer period, during which they further matured in function. BrdU incorporation indicated that cell expansion occurred at multiple stages except SP1. Nevertheless, CFSE labeling revealed that only a limited number of cells actually divided during their stay in the medulla. As to the thymic emigration, there was a clear bias toward cells with increasing maturity, but no distinction was found between dividing and nondividing thymocytes. Collectively, these data not only provide solid evidence for a highly ordered differentiation program for CD4SP thymocytes, but they also illustrate several important features associated with the developmental process.

语种英语
WOS记录号WOS:000253005600037
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被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63668
专题北京大学基础医学院_免疫学系
北京大学基础医学院
作者单位Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
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Jin, Rong,Wang, Wei,Yao, Jin-Yan,et al. Characterization of the in vivo dynamics of medullary CD4(+)CD8(-) thymocyte development[J]. JOURNAL OF IMMUNOLOGY,2008,180(4):2256-2263.
APA Jin, Rong.,Wang, Wei.,Yao, Jin-Yan.,Zhou, Yu-Bin.,Qian, Xiao-Ping.,...&Chen, Wei-Feng.(2008).Characterization of the in vivo dynamics of medullary CD4(+)CD8(-) thymocyte development.JOURNAL OF IMMUNOLOGY,180(4),2256-2263.
MLA Jin, Rong,et al."Characterization of the in vivo dynamics of medullary CD4(+)CD8(-) thymocyte development".JOURNAL OF IMMUNOLOGY 180.4(2008):2256-2263.
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