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学科主题: 基础医学
题名:
Characterization of the in vivo dynamics of medullary CD4(+)CD8(-) thymocyte development
作者: Jin, Rong; Wang, Wei; Yao, Jin-Yan; Zhou, Yu-Bin; Qian, Xiao-Ping; Zhang, Jun; Zhang, Yu; Chen, Wei-Feng
刊名: JOURNAL OF IMMUNOLOGY
发表日期: 2008-02-15
卷: 180, 期:4, 页:2256-2263
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: T-CELL DEVELOPMENT ; LYMPHOKINE SECRETION ; NEGATIVE SELECTION ; THYMIC EMIGRATION ; MOUSE THYMUS ; MATURATION ; RECEPTOR ; IDENTIFICATION ; PROLIFERATION ; EXPRESSION
英文摘要:

Our previous studies have defined a differentiation program followed by the newly generated single-positive (SP) thymocytes before their emigration to the periphery. In the present study, we further characterize the development of CD4SP cells in the thymic medulla using mainly intrathymic adoptive transfer assays. By analyzing the differentiation kinetics of the donor cells, which were shown to home correctly to the medullary region following adoptive transfer, we established the precursor-progeny relationship among the four subsets of CD4SP thymocytes (SP1-SP4) and demonstrated that the progression from SP1 to SP4 was unidirectional and largely synchronized. Notably, while the phenotypic maturation from SP1 to SP4 was achieved in 2-3 days, a small fraction of donor cells could be retained in the thymus for a longer period, during which they further matured in function. BrdU incorporation indicated that cell expansion occurred at multiple stages except SP1. Nevertheless, CFSE labeling revealed that only a limited number of cells actually divided during their stay in the medulla. As to the thymic emigration, there was a clear bias toward cells with increasing maturity, but no distinction was found between dividing and nondividing thymocytes. Collectively, these data not only provide solid evidence for a highly ordered differentiation program for CD4SP thymocytes, but they also illustrate several important features associated with the developmental process.

语种: 英语
WOS记录号: WOS:000253005600037
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63668
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Jin, Rong,Wang, Wei,Yao, Jin-Yan,et al. Characterization of the in vivo dynamics of medullary CD4(+)CD8(-) thymocyte development[J]. JOURNAL OF IMMUNOLOGY,2008,180(4):2256-2263.
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