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In vivo metabolites and plasma exposure of TongMai Keli analyzed by UHPLC/DAD/qTOF-MS and LC/MS/MS
Liu, Chun-fang1; Qiao, Xue1; Liu, Ke-di1; Miao, Wen-juan1; Li, Yan-jiao1; Liu, Yang2; Jiang, Yan-yan2; Bo, Tao3; Shi, Ren-bing2; Guo, De-an1; Ye, Min1
关键词TongMai Keli Metabolites identification Plasma exposure Pharmacokinetics UHPLC/DAD/qTOF-MS LC/MS/MS
刊名JOURNAL OF ETHNOPHARMACOLOGY
2013-01-30
DOI10.1016/j.jep.2012.11.021
145期:2页:509-516
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
资助者Ministry of Science and Technology of China ; Ministry of Science and Technology of China
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; PUERARIA-LOBATA ; C-GLYCOSIDES ; RAT PLASMA ; PHARMACOKINETICS ; DAIDZEIN ; QUANTIFICATION ; IDENTIFICATION ; CONSTITUENTS
英文摘要

Ethnopharmacological relevance: TongMai Keli (TM) is a widely used traditional Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular diseases. It is composed of Puerariae Lobatae Radix (roots of Pueraria lobata (Willd.) Ohwi), Salviae Miltiorrhizae Radix (roots of Salvia miltiorrhiza Bge.), and Chuanxiong Rhizoma (rhizomes of Ligusticum chuanxiong Hort.). The aim of this study is to identify the in vivo metabolites of TM, and to elucidate the pharmacokinetics of TM constituents and their metabolites.

Materials and methods: For metabolites identification, TM was orally administered to rats (n=3), and the metabolites in plasma were identified by UHPLC/DAD/qTOF-MS analysis and beta-glucuronidase hydrolysis. For pharmacokinetic study, rats (n=10) were treated with TM at a clinical dose, and the plasma was analyzed by LC/MS/MS.

Results: A total of 25 metabolites from TM were identified in rats plasma. Glucuronide and sulfate conjugations were the major metabolic reactions, and produced 14 metabolites. The analytical method for pharmacokinetic study was fully validated with good linearity (r>0.99), wide dynamic ranges (6-6000 ng/mL), and low variations ( < 14.3%). The plasma concentration-time curves of puerarin and nine metabolites were profiled.

Conclusion: Isoflavones from Puerariae Lobatae Radix were the major metabolites in rat plasma after oral administration of TM. Puerarin and other isoflavone glycosides could reach their first C-max. within 30 min, and were then rapidly eliminated, followed by their phase II metabolites. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

语种英语
所属项目编号2009ZX09502-006
资助者Ministry of Science and Technology of China ; Ministry of Science and Technology of China
WOS记录号WOS:000315005300011
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63673
专题北京大学药学院
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100102, Peoples R China
3.Agilent Technol, Beijing 100102, Peoples R China
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GB/T 7714
Liu, Chun-fang,Qiao, Xue,Liu, Ke-di,et al. In vivo metabolites and plasma exposure of TongMai Keli analyzed by UHPLC/DAD/qTOF-MS and LC/MS/MS[J]. JOURNAL OF ETHNOPHARMACOLOGY,2013,145(2):509-516.
APA Liu, Chun-fang.,Qiao, Xue.,Liu, Ke-di.,Miao, Wen-juan.,Li, Yan-jiao.,...&Ye, Min.(2013).In vivo metabolites and plasma exposure of TongMai Keli analyzed by UHPLC/DAD/qTOF-MS and LC/MS/MS.JOURNAL OF ETHNOPHARMACOLOGY,145(2),509-516.
MLA Liu, Chun-fang,et al."In vivo metabolites and plasma exposure of TongMai Keli analyzed by UHPLC/DAD/qTOF-MS and LC/MS/MS".JOURNAL OF ETHNOPHARMACOLOGY 145.2(2013):509-516.
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