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Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes
Xu Li1; Zhai Yao1; Lyu Yuan2; Wang Qi3; An Shuchang4; Chen Jichao5; Chen Yusheng6; Liu Lin1; Li Jiabin7; Gao Zhancheng1
关键词Klebsiella pneumoniae extended-spectrum beta-lactamase gene silencing beta-lactam resistance promoter regions
刊名CHINESE MEDICAL JOURNAL
2014-09-05
DOI10.3760/cma.j.issn.0366-6999.20140628
127期:17页:3051-3057
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]RESPIRATORY-TRACT INFECTION ; ESCHERICHIA-COLI ; CLINICAL ISOLATE ; PREVALENCE ; EXPRESSION ; ENTEROBACTERIACEAE ; SALMONELLA ; PROMOTERS ; COMMUNITY ; BLA(ESBL)
英文摘要

Background The extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae has increasingly become a major contributor to nosoconnial infections and can exhibit multiple antibiotic resistance. Previous studies have focused on the resistance genes in ESBL-producing strains, and the resistance-associated genetic environment of non-ESBL-producing strains has been ignored until now. Here, we investigated the occurrence and characteristics of non-ESBL-producing K. pneumoniae, which potentially carries unexpressed resistance genes.

Methods K. pneumoniae strains were collected from five medical institutions in China from February 2010 to August 2013. The VITEK-2 ESBL detection system was used as a primary screen to identify the ESBL-producing phenotype, and the three primary types of ESBL-associated genes (CTX, SHV, and TEM) were detected by polymerase chain reaction (PCR) to confirm the strains presenting with a non-ESBL-producing phenotype. mRNA expression in the non-ESBL-producing strains was further screened by reverse-transcription PCR (RT-PCR) to validate their transcriptional efficiency.

Results Out of 224 clinically isolated antibiotic-sensitive K. pneumoniae strains with a non-ESBL-producing phenotype, 5 (2.2%) were identified to carry inactivated ESBL bla(SHV) genes with intact upstream promoter regions and resistance gene sequences. Interestingly, three of the five antibiotic-sensitive K. pneumoniae strains containing ESBL bla(SHV) genes still exhibited mRNA transcription of bla(SHV), while the other two exhibited no mRNA transcription.

Conclusion These findings suggest that inactivated ESBL genes exist in non-ESBL-producing antibiotic-sensitive K. pneumoniae strains, which have the potential to transform the strain into an ESBL phenotype if an inappropriate application or overdose of antibiotics is implemented during clinical management.

语种英语
WOS记录号WOS:000343337100004
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63675
专题北京大学第二临床医学院
医学人文研究院/公共教学部_哲学与社会科学系
北京大学第二临床医学院_呼吸科
作者单位1.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China
2.Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing 100034, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Resp Med, Dalian 116023, Liaoning, Peoples R China
4.Tsinghua Univ, Hosp 1, Dept Resp Med, Beijing 100016, Peoples R China
5.China Aerosp Corp, Cent Hosp, Dept Resp Med, Beijing 100049, Peoples R China
6.Fujian Prov Hosp, Dept Resp Med, Fuzhou 350001, Fujian, Peoples R China
7.Anhui Med Univ, Affiliated Hosp 1, Dept Infect Dis, Hefei 230022, Anhui, Peoples R China
推荐引用方式
GB/T 7714
Xu Li,Zhai Yao,Lyu Yuan,et al. Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes[J]. CHINESE MEDICAL JOURNAL,2014,127(17):3051-3057.
APA Xu Li.,Zhai Yao.,Lyu Yuan.,Wang Qi.,An Shuchang.,...&Gao Zhancheng.(2014).Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes.CHINESE MEDICAL JOURNAL,127(17),3051-3057.
MLA Xu Li,et al."Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes".CHINESE MEDICAL JOURNAL 127.17(2014):3051-3057.
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