|Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes|
|Xu Li1; Zhai Yao1; Lyu Yuan2; Wang Qi3; An Shuchang4; Chen Jichao5; Chen Yusheng6; Liu Lin1; Li Jiabin7; Gao Zhancheng1|
|关键词||Klebsiella pneumoniae extended-spectrum beta-lactamase gene silencing beta-lactam resistance promoter regions|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||RESPIRATORY-TRACT INFECTION ; ESCHERICHIA-COLI ; CLINICAL ISOLATE ; PREVALENCE ; EXPRESSION ; ENTEROBACTERIACEAE ; SALMONELLA ; PROMOTERS ; COMMUNITY ; BLA(ESBL)|
Background The extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae has increasingly become a major contributor to nosoconnial infections and can exhibit multiple antibiotic resistance. Previous studies have focused on the resistance genes in ESBL-producing strains, and the resistance-associated genetic environment of non-ESBL-producing strains has been ignored until now. Here, we investigated the occurrence and characteristics of non-ESBL-producing K. pneumoniae, which potentially carries unexpressed resistance genes.
Methods K. pneumoniae strains were collected from five medical institutions in China from February 2010 to August 2013. The VITEK-2 ESBL detection system was used as a primary screen to identify the ESBL-producing phenotype, and the three primary types of ESBL-associated genes (CTX, SHV, and TEM) were detected by polymerase chain reaction (PCR) to confirm the strains presenting with a non-ESBL-producing phenotype. mRNA expression in the non-ESBL-producing strains was further screened by reverse-transcription PCR (RT-PCR) to validate their transcriptional efficiency.
Results Out of 224 clinically isolated antibiotic-sensitive K. pneumoniae strains with a non-ESBL-producing phenotype, 5 (2.2%) were identified to carry inactivated ESBL bla(SHV) genes with intact upstream promoter regions and resistance gene sequences. Interestingly, three of the five antibiotic-sensitive K. pneumoniae strains containing ESBL bla(SHV) genes still exhibited mRNA transcription of bla(SHV), while the other two exhibited no mRNA transcription.
Conclusion These findings suggest that inactivated ESBL genes exist in non-ESBL-producing antibiotic-sensitive K. pneumoniae strains, which have the potential to transform the strain into an ESBL phenotype if an inappropriate application or overdose of antibiotics is implemented during clinical management.
|项目编号||201202011 ; 2012ZX10004-206|
|资助机构||Special Fund for Health Scientific Research in the Public Interest Program from National Health and Family Planning Commission of China ; Ministry of Science and Technology of China|
|作者单位||1.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China|
2.Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing 100034, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Resp Med, Dalian 116023, Liaoning, Peoples R China
4.Tsinghua Univ, Hosp 1, Dept Resp Med, Beijing 100016, Peoples R China
5.China Aerosp Corp, Cent Hosp, Dept Resp Med, Beijing 100049, Peoples R China
6.Fujian Prov Hosp, Dept Resp Med, Fuzhou 350001, Fujian, Peoples R China
7.Anhui Med Univ, Affiliated Hosp 1, Dept Infect Dis, Hefei 230022, Anhui, Peoples R China
|Xu Li,Zhai Yao,Lyu Yuan,et al. Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes[J]. CHINESE MEDICAL JOURNAL,2014,127(17):3051-3057.|
|APA||Xu Li.,Zhai Yao.,Lyu Yuan.,Wang Qi.,An Shuchang.,...&Gao Zhancheng.(2014).Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes.CHINESE MEDICAL JOURNAL,127(17),3051-3057.|
|MLA||Xu Li,et al."Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes".CHINESE MEDICAL JOURNAL 127.17(2014):3051-3057.|