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学科主题: 临床医学
题名:
Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney Tumor
作者: Xu, Xun1,2; Hou, Yong1,3,4; Yin, Xuyang1; Bao, Li1; Tang, Aifa5,6; Song, Luting1; Li, Fuqiang1; Tsang, Shirley7; Wu, Kui1; Wu, Hanjie1,8; He, Weiming1; Zeng, Liang1; Xing, Manjie1; Wu, Renhua1; Jiang, Hui1; Liu, Xiao1; Cao, Dandan1; Guo, Guangwu1; Hu, Xueda1; Gui, Yaoting; Li, Zesong5,6; Xie, Wenyue9; Sun, Xiaojuan5,6; Shi, Min9; Cai, Zhiming5,6,9; Wang, Bin1; Zhong, Meiming1; Li, Jingxiang1; Lu, Zuhong3,4; Gu, Ning3,4; Zhang, Xiuqing1; Goodman, Laurie1; Bolund, Lars1,10; Wang, Jian1; Yang, Huanming1; Kristiansen, Karsten1,11; Dean, Michael1,13; Li, Yingrui1; Wang, Jun1,11,12
刊名: CELL
发表日期: 2012-03-02
DOI: 10.1016/j.cell.2012.02.025
卷: 148, 期:5, 页:886-895
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: MULTIPLE DISPLACEMENT AMPLIFICATION ; LUNG-CANCER ; SULT1A1 POLYMORPHISM ; LYSINE ACETYLATION ; RENAL-CARCINOMA ; HIF PATHWAY ; GENOME ; GENE ; EXPRESSION ; PROTEIN
英文摘要:

Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer and has very few mutations that are shared between different patients. To better understand the intratumoral genetics underlying mutations of ccRCC, we carried out single-cell exome sequencing on a ccRCC tumor and its adjacent kidney tissue. Our data indicate that this tumor was unlikely to have resulted from mutations in VHL and PBRM1. Quantitative population genetic analysis indicates that the tumor did not contain any significant clonal subpopulations and also showed that mutations that had different allele frequencies within the population also had different mutation spectrums. Analyses of these data allowed us to delineate a detailed intratumoral genetic landscape at a single-cell level. Our pilot study demonstrates that ccRCC may be more genetically complex than previously thought and provides information that can lead to new ways to investigate individual tumors, with the aim of developing more effective cellular targeted therapies.

语种: 英语
所属项目编号: 2011CB809202 ; 2011CB809203 ; 2009AA022707 ; 2012AA02A201 ; ZYC201005250020A ; CX B200903 110066A ; CXB201108250096A ; CXB200903090055A ; CXB201005250016A
项目资助者: National Basic Research Program of China (973 program) ; Chinese 863 program ; Shenzhen Municipal Government of China ; Key Laboratory Project, Shenzhen City ; Shenzhen Key Laboratory of Gene Bank for National Life Science ; Innovative Research Team of Guangdong ; Guangdong Enterprise Key Laboratory of Human Disease Genomics ; Shenzhen Key Laboratory, Shenzhen, China ; Danish Natural Science Research Council ; Danish National Research Foundation ; National Natural Science Foundation of China ; Shenzhen Municipal Government ; Local Government of Yantian District of Shenzhen ; National Cancer Institute, National Institutes of Health, USA
WOS记录号: WOS:000300985000010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63700
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.BGI Shenzhen, Shenzhen 518083, Peoples R China
2.BGI Amer, Cambridge, MA 02142 USA
3.Southeast Univ, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China
4.Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
5.Shenzhen Second Peoples Hosp, Dept Urol, Shenzhen 518035, Peoples R China
6.Shenzhen Univ, Inst Urogenital Dis, Shenzhen 518060, Peoples R China
7.BioMatrix LLC, Rockville, MD 20849 USA
8.S China Univ Technol, Sch Biosci & Biotechnol, Guangzhou 510641, Guangdong, Peoples R China
9.Peking Univ, Shenzhen PKU HKUST Med Ctr, Inst Urol,Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China
10.Univ Aarhus, Inst Human Genet, DK-8100 Aarhus, Denmark
11.Univ Copenhagen, Dept Biol, DK-1165 Copenhagen, Denmark
12.Univ Copenhagen, Novo Nordisk Fdn Ctr Basic Metab Res, DK-1165 Copenhagen, Denmark
13.NCI, Canc & Inflammat Program, NIH, Frederick, MD 21701 USA

Recommended Citation:
Xu, Xun,Hou, Yong,Yin, Xuyang,et al. Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney Tumor[J]. CELL,2012,148(5):886-895.
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