IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney Tumor
Xu, Xun1,2; Hou, Yong1,3,4; Yin, Xuyang1; Bao, Li1; Tang, Aifa5,6; Song, Luting1; Li, Fuqiang1; Tsang, Shirley7; Wu, Kui1; Wu, Hanjie1,8; He, Weiming1; Zeng, Liang1; Xing, Manjie1; Wu, Renhua1; Jiang, Hui1; Liu, Xiao1; Cao, Dandan1; Guo, Guangwu1; Hu, Xueda1; Gui, Yaoting; Li, Zesong5,6; Xie, Wenyue9; Sun, Xiaojuan5,6; Shi, Min9; Cai, Zhiming5,6,9; Wang, Bin1; Zhong, Meiming1; Li, Jingxiang1; Lu, Zuhong3,4; Gu, Ning3,4; Zhang, Xiuqing1; Goodman, Laurie1; Bolund, Lars1,10; Wang, Jian1; Yang, Huanming1; Kristiansen, Karsten1,11; Dean, Michael1,13; Li, Yingrui1; Wang, Jun1,11,12
刊名CELL
2012-03-02
DOI10.1016/j.cell.2012.02.025
148期:5页:886-895
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]MULTIPLE DISPLACEMENT AMPLIFICATION ; LUNG-CANCER ; SULT1A1 POLYMORPHISM ; LYSINE ACETYLATION ; RENAL-CARCINOMA ; HIF PATHWAY ; GENOME ; GENE ; EXPRESSION ; PROTEIN
英文摘要

Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer and has very few mutations that are shared between different patients. To better understand the intratumoral genetics underlying mutations of ccRCC, we carried out single-cell exome sequencing on a ccRCC tumor and its adjacent kidney tissue. Our data indicate that this tumor was unlikely to have resulted from mutations in VHL and PBRM1. Quantitative population genetic analysis indicates that the tumor did not contain any significant clonal subpopulations and also showed that mutations that had different allele frequencies within the population also had different mutation spectrums. Analyses of these data allowed us to delineate a detailed intratumoral genetic landscape at a single-cell level. Our pilot study demonstrates that ccRCC may be more genetically complex than previously thought and provides information that can lead to new ways to investigate individual tumors, with the aim of developing more effective cellular targeted therapies.

语种英语
WOS记录号WOS:000300985000010
Citation statistics
Cited Times:322[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63700
Collection北京大学深圳医院
作者单位1.BGI Shenzhen, Shenzhen 518083, Peoples R China
2.BGI Amer, Cambridge, MA 02142 USA
3.Southeast Univ, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China
4.Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
5.Shenzhen Second Peoples Hosp, Dept Urol, Shenzhen 518035, Peoples R China
6.Shenzhen Univ, Inst Urogenital Dis, Shenzhen 518060, Peoples R China
7.BioMatrix LLC, Rockville, MD 20849 USA
8.S China Univ Technol, Sch Biosci & Biotechnol, Guangzhou 510641, Guangdong, Peoples R China
9.Peking Univ, Shenzhen PKU HKUST Med Ctr, Inst Urol,Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China
10.Univ Aarhus, Inst Human Genet, DK-8100 Aarhus, Denmark
11.Univ Copenhagen, Dept Biol, DK-1165 Copenhagen, Denmark
12.Univ Copenhagen, Novo Nordisk Fdn Ctr Basic Metab Res, DK-1165 Copenhagen, Denmark
13.NCI, Canc & Inflammat Program, NIH, Frederick, MD 21701 USA
Recommended Citation
GB/T 7714
Xu, Xun,Hou, Yong,Yin, Xuyang,et al. Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney Tumor[J]. CELL,2012,148(5):886-895.
APA Xu, Xun.,Hou, Yong.,Yin, Xuyang.,Bao, Li.,Tang, Aifa.,...&Wang, Jun.(2012).Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney Tumor.CELL,148(5),886-895.
MLA Xu, Xun,et al."Single-Cell Exome Sequencing Reveals Single-Nucleotide Mutation Characteristics of a Kidney Tumor".CELL 148.5(2012):886-895.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Xu, Xun]'s Articles
[Hou, Yong]'s Articles
[Yin, Xuyang]'s Articles
百度学术
百度学术Similar articles in
[Xu, Xun]'s Articles
[Hou, Yong]'s Articles
[Yin, Xuyang]'s Articles
必应学术
必应学术Similar articles in
[Xu, Xun]'s Articles
[Hou, Yong]'s Articles
[Yin, Xuyang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.