北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
Redox-induced apoptosis of human oocytes in resting follicles in vitro
作者: Zhang, Xiao; Li, Xiao-Hong; Ma, Xu; Wang, Zhao-Huel; Lu, Shuang; Guo, Ying-Lu
关键词: ovary ; oocyte ; oxidative stress ; apoptosis ; resting follicle
刊名: JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
发表日期: 2006-09-01
DOI: 10.1016/j.jsgi.2006.05.005
卷: 13, 期:6, 页:451-458
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology
研究领域[WOS]: Obstetrics & Gynecology
关键词[WOS]: HUMAN OVARIAN FOLLICLES ; CELL-DEATH ; OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; POLYCYSTIC-OVARY ; GROWTH ARREST ; CYTOCHROME-C ; MITOCHONDRIA ; SURVIVAL ; AGE
英文摘要:

BACKGROUND: The age-related depletion of the resting follicle (RF) stock occurs as a result of two processes: atresia and entry in growth phase. Due to difficulties in obtaining sufficient numbers of RFs for study, little is known about the apoptotic mechanisms for RF atresia. The present study was designed to investigate the effects of oxidative stress on the apoptosis of RF oocytes.

METHODS: RFs isolated from human adult ovaries were cultured in vitro, treated with H2O2 at various concentrations (50 mu M, 100 mu M, 1. 0 mM) for 1 hour) and observed for up to 48 hours. The oxidant-induced apoptosis of oocytes were observed by detection of DNA fragments, mitochondria membrane potential (NIMP), and cytochrome c release.

RESULTS: Based on nuclear morphology and TUNEL (terminal deoxynucleotidyl transferase-mediated dDTP nick end-labeling), oocyte apoptosis was observed in the RFs treated with 50 mu M H2O2 With rates of 35% and 43% at 24 and 48 h after treatment, respectively. But intensive oxidative stress (1 mM H2O2) caused mainly necrosis as measured by quantifying propidium iodide (PI)-positive oocytes (44% within 12 hours), with lower level of apoptosis (17%) being observed at 24 hours after treatment. RFs treated with 100 mu M H2O2 showed both apoptosis with the similar rate observed at 50 mu M and necrosis (13% PI-positive oocytes). Although pre-incubation with cyclosporine A (CsA) could effectively prevent oxidant-induced MMP collapse, but failed to suppress apoptosis of oocytes in RFs.

CONCLUSIONS: Oocytes of RFs in adult ovaries retain their ability to undergo apoptosis under oxidative stress, which is both dose- and time-dependent.

语种: 英语
WOS记录号: WOS:000240528100010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63705
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Inst Urol, Hosp 1, Beijing, Peoples R China
2.WHO, Collaborat Ctr Res Human Reprod, Beijing, Peoples R China
3.Natl Res Inst Family Planning, Beijing 100081, Peoples R China
4.Peking Univ, Reprod & Genet Ctr, Hosp 1, Beijing, Peoples R China
5.Peking Union Med Coll, Beijing, Peoples R China

Recommended Citation:
Zhang, Xiao,Li, Xiao-Hong,Ma, Xu,et al. Redox-induced apoptosis of human oocytes in resting follicles in vitro[J]. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION,2006,13(6):451-458.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Xiao]'s Articles
[Li, Xiao-Hong]'s Articles
[Ma, Xu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Xiao]‘s Articles
[Li, Xiao-Hong]‘s Articles
[Ma, Xu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace