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学科主题: 临床医学
题名:
Impact of gonadotropins on oocyte maturation, fertilisation and developmental competence in vitro
作者: Wang, Xuemei1,2; Tsai, Tony1; Qiao, Jie3; Zhang, Zhan2; Feng, Huai L.1,3
关键词: early embryo development ; in vitro maturation ; IVF
刊名: REPRODUCTION FERTILITY AND DEVELOPMENT
发表日期: 2014
DOI: 10.1071/RD13024
卷: 26, 期:5, 页:752-757
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Reproductive Biology ; Zoology
研究领域[WOS]: Developmental Biology ; Reproductive Biology ; Zoology
关键词[WOS]: LUTEINIZING-HORMONE ; OVARIAN STIMULATION ; CHORIONIC-GONADOTROPIN ; ASSISTED REPRODUCTION ; RECOMBINANT FSH ; IVF PATIENTS ; HP-HMG ; EMBRYOS ; FOLLICLES ; HCG
英文摘要:

The aim of the present study was to evaluate the dose-dependent effects of gonadotropins, either singly (Bravelle (B), Luveris (L), Menupur (M), Repronex (R), Gonal-F (G), Follism (F) and Norvarel (N)) or in combination (Menupur+Bravelle; Repronext+Bravelle; and Bravelle+Norvarel), on rates of oocyte maturation, fertilisation and early embryo development in vitro in an animal model. Bovine cumulus-oocyte complexes (COCs) were purchased commercially and cultured in TCM-199 with 10% fetal bovine serum supplemented with varying concentrations of gonadotropin (0, 5, 10, 20, 40 IU or United States Pharmacopoeia (USP) mL(-1)) for 24 and 48 h according to current IVF clinical stimulation protocols. All gonadotropins enhanced oocyte maturation in vitro in a dose-dependent manner. Individually, Gonal-F (Merck KGaA, Darmstadt, Germany), Follism (Merck Co, Whitehouse Station, NJ, USA) and Repronext (Ferring, Parsippany, NJ, USA) promoted oocyte maturation; in combination, they effectively enhanced COC expansion and increased the maturation competence of MII oocytes. However, high concentrations of gonadotropins may result in maturation arrest. Specific combinations of gonadotropins may change the rate of early embryonic development (8-16-cells) and morula-blastocyst formation. These data provide support for the responsiveness of bovine oocytes to gonadotropins in vitro and the need to consider variations in the relative concentrations and ratio of combinations (FSH/LH or human chorionic gonadotropin) for optimisation of oocyte developmental competence. The results of the present study could be applied to therapeutic clinical stimulation protocols and help improve IVF success rates.

语种: 英语
所属项目编号: 31128013/C120205 ; 39252
项目资助者: China NSFC ; Merck and Co. Inc. (North Wales, PA, USA)
WOS记录号: WOS:000337730500013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63724
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Cornell Univ, Dept Obstet & Gynecol, New York Hosp Queens, Weill Med Coll, New York, NY 11355 USA
2.Zhengzhou Univ, Reprod Med Ctr, Affiliated Hosp 3, Zhengzhou 450052, Henan, Peoples R China
3.Peking Univ, Dept Obstet & Gynecol, Hosp 3, Beijing 100083, Peoples R China

Recommended Citation:
Wang, Xuemei,Tsai, Tony,Qiao, Jie,et al. Impact of gonadotropins on oocyte maturation, fertilisation and developmental competence in vitro[J]. REPRODUCTION FERTILITY AND DEVELOPMENT,2014,26(5):752-757.
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