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学科主题: 临床医学
题名:
Evaluation of germ-cell kinetics in infertile patients with proliferating cell nuclear antigen proliferating index
作者: Zeng, L; Kong, XT; Su, JW; Xia, TL; Na, YQ; Guo, YL
关键词: male infertility ; testis ; kinetics ; proliferating cell nuclear antigen
刊名: ASIAN JOURNAL OF ANDROLOGY
发表日期: 2001-03-01
卷: 3, 期:1, 页:63-66
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology ; Urology & Nephrology
研究领域[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]: CYCLIN
英文摘要:

Aim: To explore the usefulness of proliferating cell nuclear antigen proliferating index (PCNA PI) in the pathological diagnosis and treatment of male infertility. Methods: Testicular biopsy specimen obtained from 48 cases of male infertility and 2 normal controls were fixed and embedded. The sections were stained with anti-PCNA monoclonal antibodies or haematoxylin/eosin. proliferating index (PI), expressed as the percentage of germ-cell nuclei positively stained with PCNA antibody, was assessed from more than 20 seminiferous tubules or 600 germ-cells. Results: The infertile patients were divided into 4 groups: Group 1, normal spermatogenesis (14 cases); Group 2, hypospermatogenesis (16 cases); Group 3, germinal arrest (10 cases); Group 4, Sertoli cell only syndrome (8 cases). The PCNA PI of normal control testis was 86.5% (mean value). Group 3 had a significantly lower PCNA PI (29.8%) than normal testis; Group 1 and 2 had similar PIs (82.3% and 82.3%, respectively) as the control testis. PI of the negative control (Group 4) was 0 as no germ-cells were found. Conclusion: PCNA PI is useful for assessing germ-cell kinetics, especially for pathological diagnosis of germinal arrest which is difficult to differentiate by routine HE staining technique. In germinal arrest, there is a significantly lowered PCNA PI, which is an indication of DNA synthesis deterioration, suggesting the use of therapies be different from those for hypospermatogenesis.

语种: 英语
WOS记录号: WOS:000168027100010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63725
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: Peking Univ, Inst Urol, Beijing 100034, Peoples R China

Recommended Citation:
Zeng, L,Kong, XT,Su, JW,et al. Evaluation of germ-cell kinetics in infertile patients with proliferating cell nuclear antigen proliferating index[J]. ASIAN JOURNAL OF ANDROLOGY,2001,3(1):63-66.
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