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学科主题: 临床医学
题名:
Comparison of exendin-4 on beta-cell replication in mouse and human islet grafts
作者: Tian, Lei1,2,3; Gao, Jie1,2,4; Weng, Guangbin1,2,5; Yi, Huimin1,2,6; Tian, Bole1,2,7; O′ Brien, Timothy D.8; Guo, Zhiguang1,2
关键词: beta-cell regeneration ; beta-cell replication ; exendin-4 ; GLP-1 ; islet transplantation
刊名: TRANSPLANT INTERNATIONAL
发表日期: 2011-08-01
DOI: 10.1111/j.1432-2277.2011.01275.x
卷: 24, 期:8, 页:856-864
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Surgery ; Transplantation
研究领域[WOS]: Surgery ; Transplantation
关键词[WOS]: DIABETIC NOD MICE ; FRESH ISLETS ; TRANSPLANTATION ; TURNOVER ; PROLIFERATION ; REGENERATION ; APOPTOSIS ; MASS ; AGE ; CHIMERISM
英文摘要:

Exendin-4 can stimulate beta-cell replication in mice. Whether it can stimulate beta-cell replication in human islet grafts remains unknown. Therefore, we compared the effects of exendin-4 on beta-cell replication in mouse and human islet grafts. Islets, isolated from mouse and human donors at different ages, were transplanted into diabetic mice and/or diabetic nude mice that were given bromodeoxyuridine ( BrdU) with or without exendin-4. At 4 weeks post-transplantation, islet grafts were removed for insulin and BrdU staining and quantification of insulin(+)/BrdU(+) cells. Although diabetes was reversed in all mice transplanting syngeneic mouse islets from young or old donors, normoglycemia was achieved significantly faster in exendin-4 treated mice. Mouse islet grafts in exendin-4 treated mice had significantly more insulin (+)/BrdU(+) beta cells than in untreated mice (P < 0.01). Human islet grafts from <= 22-year-old donors had more insulin (+)/BrdU(+) beta cells in exendin-4 treated mice than that in untreated mice (P < 0.01). However, human islet grafts from >= 35-year-old donors contained few insulin (+)/BrdU(+) beta cells in exendin-4 treated or untreated mice. Our data demonstrated that the capacity for beta-cell replication in mouse and human islet grafts is different with and without exendin-4 treatment and indicated that GLP-1 agonists can stimulate beta-cell replication in human islets from young donors.

语种: 英语
项目资助者: JDRF
WOS记录号: WOS:000292462000017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63733
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Univ Minnesota, Dept Surg, Minneapolis, MN 55455 USA
2.Univ Minnesota, Schulze Diabet Inst, Minneapolis, MN 55455 USA
3.Guangxi Med Univ, Affiliated Hosp 1, Dept Surg, Nanning, Peoples R China
4.Peking Univ, Peoples Hosp, Dept Hepatobilary Surg, Beijing 100871, Peoples R China
5.Ningbo Univ, Yinzhou Hosp 2, Dept Urol, Ningbo 315211, Zhejiang, Peoples R China
6.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Surg, Guangzhou 510275, Guangdong, Peoples R China
7.Sichuan Univ, W China Hosp, Dept Surg, Chengdu 610064, Peoples R China
8.Univ Minnesota, Dept Vet Populat Med, Minneapolis, MN 55455 USA

Recommended Citation:
Tian, Lei,Gao, Jie,Weng, Guangbin,et al. Comparison of exendin-4 on beta-cell replication in mouse and human islet grafts[J]. TRANSPLANT INTERNATIONAL,2011,24(8):856-864.
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