北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 北京大学人类疾病基因研究中心  > 期刊论文
学科主题: 基础医学
题名:
Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells
作者: Zhu, Xiaohui1; Wu, Lina1; Zhang, Li1; Hao, Peng1; Zhang, Shuai1; Huang, Jing1; Zheng, Jie1; Liu, Yinan2; Li, Wenjun1; Zhang, Yingmei1; Zhou, Chunyan2; Zhang, Youhui3,4; Yin, C. Cameron5; Qiu, Xiaoyan1
关键词: Ig gene transcription ; Oct-1 ; promoter ; transcription regulation ; VH4-59
刊名: CELLULAR & MOLECULAR IMMUNOLOGY
发表日期: 2010-07-01
DOI: 10.1038/cmi.2010.13
卷: 7, 期:4, 页:279-287
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: CHAIN CONSTANT-REGION ; KAPPA-LIGHT-CHAIN ; HEAVY-CHAIN ; TUMOR-CELLS ; EXPRESSION ; PROMOTER ; COACTIVATOR ; OBF-1 ; E2A ; ACTIVATION
英文摘要:

The restriction of immunoglobulin (Ig) expression to B lymphocytes is well established. However, several reports have confirmed that the Ig gene can be expressed in many non-B cancer cells and/or some normal cells. Our aim is to determine whether the Ig gene promoter can be activated in non-B cancer cells and to identify the regulatory mechanism for Ig gene expression. Our results show that the Ig promoter of VH4-59 was activated in several non-B cancer cell lines. Moreover, two novel positive regulatory elements, an enhancer-like element at -800 to -610 bp and a copromoter-like element at -610 to -300 bp, were identified in two epithelial cancer cell lines, HeLa S3 and HT-29. The octamer element (5′-ATGCAAAT-3′) located in the Ig promoter, a crucial element for B-cell-derived Ig gene transcription, was also very important for non-B-cell-derived Ig gene transcription. More importantly, we confirmed that octamer-related protein-1 (Oct-1), but not Oct-2, was a crucial transcriptional factor for Ig gene transcription due to its ability to bind to the octamer element of the Ig promoter in epithelial cancer cells. These results suggested the presence of a distinct regulatory mechanism for Ig gene expression in non-B cancer cells. Cellular & Molecular Immunology (2010) 7, 279-287; doi:10.1038/cmi.2010.13; published online 17 May 2010

语种: 英语
所属项目编号: 30572094 ; 30772470
项目资助者: Natural Sciences Foundation, China
WOS记录号: WOS:000279487500007
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63739
Appears in Collections:基础医学院_北京大学人类疾病基因研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells.pdf(858KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem, Beijing 100191, Peoples R China
3.Chinese Acad Med Sci, Inst Canc, Dept Immunol, Beijing 100021, Peoples R China
4.Peking Union Med Coll, Beijing 100021, Peoples R China
5.Univ Texas MD Anderson Canc Ctr, Dept Hematopathol, Houston, TX 77030 USA

Recommended Citation:
Zhu, Xiaohui,Wu, Lina,Zhang, Li,et al. Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells[J]. CELLULAR & MOLECULAR IMMUNOLOGY,2010,7(4):279-287.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhu, Xiaohui]'s Articles
[Wu, Lina]'s Articles
[Zhang, Li]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhu, Xiaohui]‘s Articles
[Wu, Lina]‘s Articles
[Zhang, Li]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace