北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心  > 期刊论文
学科主题: 临床医学
题名:
High FSH decreases the developmental potential of mouse oocytes and resulting fertilized embryos, but does not influence offspring physiology and behavior in vitro or in vivo
作者: Li, Min1,2; Zhao, Yue1,3; Zhao, Cui H.1,2; Yan, Jie1,2; Yan, Ying L.1,3; Rong, Li1,2; Liu, Ping1,3; Feng, Huai-Liang3,4,5; Yu, Yang1,2; Qiao, Jie1,2,3
关键词: follicle-stimulating hormone ; spindle assembly ; embryo development ; physiology index ; behavior index
刊名: HUMAN REPRODUCTION
发表日期: 2013-05-01
DOI: 10.1093/humrep/det014
卷: 28, 期:5, 页:1309-1323
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: FOLLICLE-STIMULATING-HORMONE ; INTRACYTOPLASMIC SPERM INJECTION ; INCREASES ANEUPLOIDY ; NUCLEAR TRANSFER ; GENE-EXPRESSION ; BIRTH-WEIGHT ; MATURATION ; SUPEROVULATION ; RECOMBINANT ; COMPETENCE
英文摘要:

Do different concentrations of FSH in the assisted reproductive technology (ART) procedure in vitro or in vivo affect the developmental competence of oocytes, the embryos and the offspring conceived from these embryos?

Improper FSH treatment (200 IU/l in vitro, 10 IU/ml in vivo and 200 IU/ml in vivo) impairs the development competence of oocyte and embryo, but does not influence offspring physiology and behavior.

Exogenous FSH has been widely used in the field of ART. However, the effects of different concentrations of FSH on the developmental competence of oocytes, embryos and the offspring conceived from these embryos, are still unknown.

In a prospective study, a total of 45 mice at 810 weeks of age were primed in vivo with different dosages of FSH (9 mice in the 10 IU/ml, 10 mice in the 50 IU/ml, 10 mice in the 100 IU/ml and 16 mice in the 200 IU/ml groups). Fresh MII oocytes were retrieved from ovaries: this was designated as in vivo group. Thirty six mice at 810 weeks of age were sacrificed by cervical dislocation to obtain ovaries without FSH treatment (9 mice in the 0 IU/l, 9 mice in the 50 IU/l, 8 mice in the 100 IU/l and 10 mice in the 200 IU/l groups), and then the immature oocytes were collected from these ovaries and cultured in vitro matured medium supplemented with 0, 50, 100 and 200 IU/l FSH: this was designated as in vitro group.

Spindle assembly of matured MII oocytes was stained via an immunofluorescence method and the oocytes ratio of normal spindle was analyzed. The developmental competence of the resulting fertilized embryos in the pre- and post-implantation stages was examined in in vitro and in vivo groups. Furthermore, physiological index, including reproductive potential and body weight, of the offspring was investigated by mating experiments and behavior index, including learning, memory, probing and intelligence, was tested by Morris water maze in in vitro and in vivo groups.

In the in vitro groups, the oocyte maturation competence, normal spindle assembly, blastocyst formation and implantation, as well as viable pup production were all impaired in the group treated with 200 IU/l FSH (P 0.05). No differences were observed among the other three groups (P 0.05). In the in vivo groups, 10 IU/ml FSH but not 200 IU/ml treatment influenced blastocyst formation and viable pup production (P 0.05), although the high proportion of spindle assembly abnormality was only observed in the 200 IU/ml FSH treatment group (P 0.05). Furthermore, there were no significant differences in terms of physiological index (reproductive potential and body weight) and behavior index (learning, memory, probing and intelligence) in offspring from in vitro and in vivo groups (P 0.05).

The mouse model was used in this study. The results of the mouse follicle growth and oocyte development in responding to different concentrations of FSH are not 100 transferable to human, because of the physiological differences between mouse and human.

The findings indicated that FSH application in the field of ART is safe to the resulted offspring, but it should be more carefully used for each women in ART cycles because the inappropriate FSH concentration would decrease the oocyte developmental competence.

This work was partially supported by the Ministry of Science and Technology of China Grants (973 program; 2011CB944504), the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (30825038), the National Natural Science Funds for Young Scholar (31000661) and by the Joint Research Fund for Overseas, Hong Kong and Marco Scholars (31128013/C120205). None of the authors has any conflicts of interest.

语种: 英语
所属项目编号: 2011CB944504 ; 30825038 ; 31000661 ; 31128013/C120205
项目资助者: Ministry of Science and Technology of China ; Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China ; National Natural Science Funds for Young Scholar ; Joint Research Fund for Overseas, Hong Kong and Marco Scholars
WOS记录号: WOS:000318108900019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63744
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Dept Obstet & Gynecol, Ctr Reprod Med, Hosp 3, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
4.Cornell Univ, Weill Med Coll, New York Hosp Queens, Dept Obstet & Gynecol, New York, NY 10021 USA
5.Cornell Univ, Weill Med Coll, New York Hosp Queens, Dept Lab Med, New York, NY 10021 USA

Recommended Citation:
Li, Min,Zhao, Yue,Zhao, Cui H.,et al. High FSH decreases the developmental potential of mouse oocytes and resulting fertilized embryos, but does not influence offspring physiology and behavior in vitro or in vivo[J]. HUMAN REPRODUCTION,2013,28(5):1309-1323.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Min]'s Articles
[Zhao, Yue]'s Articles
[Zhao, Cui H.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Min]‘s Articles
[Zhao, Yue]‘s Articles
[Zhao, Cui H.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace