IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Tumor suppressor gene RBM5 delivered by attenuated Salmonella inhibits lung adenocarcinoma through diverse apoptotic signaling pathways
Shao, Chen1; Yang, Baoxue4; Zhao, Lijing2,3; Wang, Song5; Zhang, Jie1; Wang, Ke1
关键词RBM5 Lung adenocarcinoma Apoptosis A549 Xenograft mice model Attenuated Salmonella
刊名WORLD JOURNAL OF SURGICAL ONCOLOGY
2013-05-31
DOI10.1186/1477-7819-11-123
11
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Surgery
研究领域[WOS]Oncology ; Surgery
关键词[WOS]HUMAN-CHROMOSOME 3P21.3 ; IN-VIVO ; CANCER CELLS ; CD95-MEDIATED APOPTOSIS ; HOMOZYGOUS DELETION ; DOWN-REGULATION ; SOLID TUMORS ; GROWTH ; RNA ; EXPRESSION
英文摘要

Background: RBM5 (RNA-binding motif protein 5, also named H37/LUCA-15) gene from chromosome 3p21.3 has been demonstrated to be a tumor suppressor. Current researches in vitro confirm that RBM5 can suppress the growth of lung adenocarcinoma cells by inducing apoptosis. There is still no effective model in vivo, however, that thoroughly investigates the effect and molecular mechanism of RBM5 on lung adenocarcinoma.

Method: We established the transplanted tumor model on BALB/c nude mice using the A549 cell line. The mice were treated with the recombinant plasmids carried by attenuated Salmonella to induce the overexpression of RBM5 in tumor tissues. RBM5 overexpression was confirmed by immunohistochemistry staining. H&E staining was performed to observe the histological performance on plasmids-treated A549 xenografts. Apoptosis was assessed by TUNEL staining with a TUNEL detection kit. Apoptosis-regulated genes were detected by Western blot.

Results: We successful established the lung adenocarcinoma animal model in vivo. The growth of tumor xenografts was significantly retarded on the mice treated with pcDNA3.1-RBM5 carried by attenuated Salmonella compared to that on mice treated with pcDNA3.1. Overexpression of RBM5 enhanced the apoptosis in tumor xenografts. Furthermore, the expression of Bcl-2 protein was decreased significantly, while the expression of BAX, TNF-alpha, cleaved caspase-3, cleaved caspase-8, cleaved caspase-9 and cleaved PARP proteins was significantly increased in the pcDNA3.1-RBM5-treated mice as compared to that in the control mice.

Conclusions: In this study, we established a novel animal model to determine RBM5 function in vivo, and concluded that RBM5 inhibited tumor growth in mice by inducing apoptosis. The study suggests that although RBM5′s involvement in the death receptor-mediated apoptotic pathway is still to be investigated, RBM5-mediated growth suppression, at least in part, employs regulation of the mitochondrial apoptotic pathways.

语种英语
WOS记录号WOS:000319982600001
项目编号81071919 ; 30971315 ; 201015169
资助机构National Natural Science Foundation of China ; Provincial Natural Science Foundation of Jilin
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63746
专题北京大学基础医学院_药理学系
北京大学基础医学院
作者单位1.Jilin Univ, Dept Resp Med, Affiliated Hosp 2, Changchun 130041, Jilin, Peoples R China
2.Jilin Univ, China Japan Union Hosp, Dept Digest Med, Changchun 130033, Jilin, Peoples R China
3.Jilin Univ, Norman Bethune Coll Med, Dept Pathophysiol, Changchun 130021, Jilin, Peoples R China
4.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100191, Peoples R China
5.Jilin Univ, Dept Urinary Surg, Affiliated Hosp 1, Changchun 130041, Jilin, Peoples R China
推荐引用方式
GB/T 7714
Shao, Chen,Yang, Baoxue,Zhao, Lijing,et al. Tumor suppressor gene RBM5 delivered by attenuated Salmonella inhibits lung adenocarcinoma through diverse apoptotic signaling pathways[J]. WORLD JOURNAL OF SURGICAL ONCOLOGY,2013,11.
APA Shao, Chen,Yang, Baoxue,Zhao, Lijing,Wang, Song,Zhang, Jie,&Wang, Ke.(2013).Tumor suppressor gene RBM5 delivered by attenuated Salmonella inhibits lung adenocarcinoma through diverse apoptotic signaling pathways.WORLD JOURNAL OF SURGICAL ONCOLOGY,11.
MLA Shao, Chen,et al."Tumor suppressor gene RBM5 delivered by attenuated Salmonella inhibits lung adenocarcinoma through diverse apoptotic signaling pathways".WORLD JOURNAL OF SURGICAL ONCOLOGY 11(2013).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Tumor suppressor gen(1103KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Shao, Chen]的文章
[Yang, Baoxue]的文章
[Zhao, Lijing]的文章
百度学术
百度学术中相似的文章
[Shao, Chen]的文章
[Yang, Baoxue]的文章
[Zhao, Lijing]的文章
必应学术
必应学术中相似的文章
[Shao, Chen]的文章
[Yang, Baoxue]的文章
[Zhao, Lijing]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Tumor suppressor gene RBM5 delivered by attenuated Salmonella inhibits lung adenocarcinoma through diverse apoptotic signaling pathways.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。