北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心  > 期刊论文
学科主题: 临床医学
题名:
Effects of tumor necrosis factor-alpha on porcine oocyte meiosis progression, spindle organization, and chromosome alignment
作者: Ma, Cai-Hong1; Yan, Li-Ying1; Qiao, Jie1; Sha, Wei2; Li, Li1; Chen, Yuan1; Sun, Qing-Yuan3
关键词: Endometriosis ; TNF-alpha ; oocyte maturation ; microtubules ; chromosome alignment
刊名: FERTILITY AND STERILITY
发表日期: 2010-02-01
DOI: 10.1016/j.fertnstert.2009.01.131
卷: 93, 期:3, 页:920-926
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: FOLLICULAR-FLUID ; MOUSE OOCYTE ; ENDOMETRIOSIS ; MATURATION ; VITRIFICATION ; FOLLICLES ; APOPTOSIS ; QUALITY ; MARKERS
英文摘要:

Objective: To evaluate the effects of tumor necrosis factor-alpha (TNF-alpha) on porcine oocyte maturation, spindle dynamics, and chromosome alignment.

Design: Controlled, prospective study.

Setting: University hospital and IVF research laboratory.

Animal(s): Ovaries collected from slaughtered prepubertal gilts, Main Outcome Measure(s): Oocyte maturation rate and cytoskeleton distribution.

Materials and Method(s): Immature porcine oocytes (GV) were exposed to TNF-alpha at a concentration of 0 (as a control), 1, 5, 10, 100, 200, or 600 ng/mL in M199 medium. Oocytes were cultured for 24 hours to the pre-MI stage or 44 hours to the MII stage. After in vitro maturation for 44 hours, the rates of GV oocytes reaching MII stage were assessed, and MII oocytes were fixed for further examination of the cytoskeleton and the chromosomal distribution.

Result(s): The TNF-alpha concentration at 5 ng/mL decreased the porcine oocyte maturation rate compared with the control after culture for 44 hours, whereas exposure to 10 or 100 ng/mL TNF-alpha resulted in a significant increase in the frequency of defective spindles or abnormal microfilament distribution. Exposed to 200 ng/mL, TNF-alpha caused a significantly higher abnormality rate of chromosome alignment when compared with the controls.

Conclusion(S): Exposure of porcine oocytes to ail elevated TNF-alpha concentration clearly caused a reduction in their maturation from GV stage to MII stage and increased the proportion of oocytes with abnormal chromosome alignment and cytoskeleton structure. (Fertil Steril (R) 2010;93:920-6. (C)2010 by American Society For Reproductive Medicine.)

语种: 英语
WOS记录号: WOS:000274957300035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63757
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Ctr Reprod Med, Dept Obstet & Gynecol, Hosp 3, Beijing 100083, Peoples R China
2.NE Agr Univ, Dept Anim Sci & Technol, Harbin, Peoples R China
3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China

Recommended Citation:
Ma, Cai-Hong,Yan, Li-Ying,Qiao, Jie,et al. Effects of tumor necrosis factor-alpha on porcine oocyte meiosis progression, spindle organization, and chromosome alignment[J]. FERTILITY AND STERILITY,2010,93(3):920-926.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ma, Cai-Hong]'s Articles
[Yan, Li-Ying]'s Articles
[Qiao, Jie]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ma, Cai-Hong]‘s Articles
[Yan, Li-Ying]‘s Articles
[Qiao, Jie]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace