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Effects of tumor necrosis factor-alpha on porcine oocyte meiosis progression, spindle organization, and chromosome alignment
Ma, Cai-Hong1; Yan, Li-Ying1; Qiao, Jie1; Sha, Wei2; Li, Li1; Chen, Yuan1; Sun, Qing-Yuan3
关键词Endometriosis TNF-alpha oocyte maturation microtubules chromosome alignment
刊名FERTILITY AND STERILITY
2010-02-01
DOI10.1016/j.fertnstert.2009.01.131
93期:3页:920-926
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]FOLLICULAR-FLUID ; MOUSE OOCYTE ; ENDOMETRIOSIS ; MATURATION ; VITRIFICATION ; FOLLICLES ; APOPTOSIS ; QUALITY ; MARKERS
英文摘要

Objective: To evaluate the effects of tumor necrosis factor-alpha (TNF-alpha) on porcine oocyte maturation, spindle dynamics, and chromosome alignment.

Design: Controlled, prospective study.

Setting: University hospital and IVF research laboratory.

Animal(s): Ovaries collected from slaughtered prepubertal gilts, Main Outcome Measure(s): Oocyte maturation rate and cytoskeleton distribution.

Materials and Method(s): Immature porcine oocytes (GV) were exposed to TNF-alpha at a concentration of 0 (as a control), 1, 5, 10, 100, 200, or 600 ng/mL in M199 medium. Oocytes were cultured for 24 hours to the pre-MI stage or 44 hours to the MII stage. After in vitro maturation for 44 hours, the rates of GV oocytes reaching MII stage were assessed, and MII oocytes were fixed for further examination of the cytoskeleton and the chromosomal distribution.

Result(s): The TNF-alpha concentration at 5 ng/mL decreased the porcine oocyte maturation rate compared with the control after culture for 44 hours, whereas exposure to 10 or 100 ng/mL TNF-alpha resulted in a significant increase in the frequency of defective spindles or abnormal microfilament distribution. Exposed to 200 ng/mL, TNF-alpha caused a significantly higher abnormality rate of chromosome alignment when compared with the controls.

Conclusion(S): Exposure of porcine oocytes to ail elevated TNF-alpha concentration clearly caused a reduction in their maturation from GV stage to MII stage and increased the proportion of oocytes with abnormal chromosome alignment and cytoskeleton structure. (Fertil Steril (R) 2010;93:920-6. (C)2010 by American Society For Reproductive Medicine.)

语种英语
WOS记录号WOS:000274957300035
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63757
专题北京大学第三临床医学院_妇产科
北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Ctr Reprod Med, Dept Obstet & Gynecol, Hosp 3, Beijing 100083, Peoples R China
2.NE Agr Univ, Dept Anim Sci & Technol, Harbin, Peoples R China
3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Ma, Cai-Hong,Yan, Li-Ying,Qiao, Jie,et al. Effects of tumor necrosis factor-alpha on porcine oocyte meiosis progression, spindle organization, and chromosome alignment[J]. FERTILITY AND STERILITY,2010,93(3):920-926.
APA Ma, Cai-Hong.,Yan, Li-Ying.,Qiao, Jie.,Sha, Wei.,Li, Li.,...&Sun, Qing-Yuan.(2010).Effects of tumor necrosis factor-alpha on porcine oocyte meiosis progression, spindle organization, and chromosome alignment.FERTILITY AND STERILITY,93(3),920-926.
MLA Ma, Cai-Hong,et al."Effects of tumor necrosis factor-alpha on porcine oocyte meiosis progression, spindle organization, and chromosome alignment".FERTILITY AND STERILITY 93.3(2010):920-926.
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