学科主题基础医学
Oct4 is expressed in Nestin-positive cells as a marker for pancreatic endocrine progenitor
Wang, Hong2; Wang, Shuling2; Hu, Jiang2; Kong, Yaxian2; Chen, Shen2; Li, Lingsong; Li, Liying1,2
关键词Oct4 Ngn3 Nestin Fetal pancreas
刊名HISTOCHEMISTRY AND CELL BIOLOGY
2009-05-01
DOI10.1007/s00418-009-0560-x
131期:5页:553-563
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Microscopy
研究领域[WOS]Cell Biology ; Microscopy
关键词[WOS]EMBRYONIC STEM-CELLS ; DEVELOPING MOUSE PANCREAS ; TRANSCRIPTION FACTORS ; GENE-EXPRESSION ; C-KIT ; DIFFERENTIATION ; EXOCRINE ; ISLET ; NEUROGENIN3 ; PRECURSORS
英文摘要

There are abundant progenitor cells in the developing pancreas, but molecular markers for these cells are lacking. Octamer-binding transcription factor-4 (Oct4) is an important transcription factor for keeping the features of self-renewal and pluripotency of embryonic stem cells. It′s well known that Oct4, as a totipotent stem cells marker, just is expressed in totipotent stem cells. In the present study, we collected ten human fetal pancreases, and found that Oct4 mRNA and protein were expressed in human fetal pancreas samples by RT-PCR, western blot and immunohistochemistry assays. Using double-staining, we demonstrated that Oct4 was not co-expressed with Chromogranin A (a peptide expressed in endocrine cells), but partially co-expressed with Ngn3 (a transcription factor expressed in pancreatic endocrine precursor cells) and Nestin (a intermediate filament, Nestin-positive cells isolated from islets can be induced to express insulin) in human fetal pancreases. Indeed, we prepared Nestin-positive cells from human fetal pancreas by cell selection, and found that these cells expressed Oct4 and Ngn3. The Nestin-positive cells displayed a rapid duplication and could differentiate into osteoblasts, fat and endocrine cells in vitro. These results indicated that the Nestin-positive cells in the fetal age should be pancreatic progenitor cells. Overall, our study suggested that Oct4 was a marker for pancreatic endocrine progenitor.

语种英语
WOS记录号WOS:000264811100002
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63766
专题北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Capital Med Univ, Dept Cell Biol, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100871, Peoples R China
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GB/T 7714
Wang, Hong,Wang, Shuling,Hu, Jiang,et al. Oct4 is expressed in Nestin-positive cells as a marker for pancreatic endocrine progenitor[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2009,131(5):553-563.
APA Wang, Hong.,Wang, Shuling.,Hu, Jiang.,Kong, Yaxian.,Chen, Shen.,...&Li, Liying.(2009).Oct4 is expressed in Nestin-positive cells as a marker for pancreatic endocrine progenitor.HISTOCHEMISTRY AND CELL BIOLOGY,131(5),553-563.
MLA Wang, Hong,et al."Oct4 is expressed in Nestin-positive cells as a marker for pancreatic endocrine progenitor".HISTOCHEMISTRY AND CELL BIOLOGY 131.5(2009):553-563.
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