学科主题基础医学
Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells
Li, Qi1,2; Wang, Li1,2; Zeng, Linge1,2; Zhang, Yingmei1,2; Li, Kangqi1,2; Lin, Peng1,2; Su, Bingnan2; Wang, Lu1,2
关键词Rab apoptosis motility focal adhesion kinase paclitaxel
刊名ONCOLOGY REPORTS
2010-08-01
DOI10.3892/or_00000876
24期:2页:433-440
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National Natural Science Foundation of China ; National High Biotechnology Development Program of China ; National Natural Science Foundation of China ; National High Biotechnology Development Program of China
研究领域[WOS]Oncology
关键词[WOS]PROTEIN ; PACLITAXEL ; TAXOL ; FAK ; INHIBITION ; MIGRATION ; TRANSPORT ; PATHWAY ; GTPASES ; OVARIAN
英文摘要

Rab is a large subfamily of small GTPases which play an important role in multiple processes relating to cellular transportation and modulation of the cytoskeleton. Novel gene Rab-like 3 (Rabl3), which we originally reported as a new member belonging to this subfamily, may participate in other processes in human cancer cell lines based on our research. In order to investigate the function of Rabl3 and the basic mechanism which regulates cancer cell survival and motility, we constructed the Rab13-pcDNA3.1 fusion plasmid, also, the specific siRNA against Rabl3 was used. We detected cellular viability and motility changes in Rabl3 overexpressed or si-Rabl3 transfected cancer cell lines. Overexpression of Rabl3 resulted in the enhancement of cell proliferation, inhibition of apoptosis and paclitaxel resistance in human cancer cell lines. Rabl3 also promoted cell motility activity. Next, we silenced Rabl3 in order to determine its exact physiological function. We found that processes which are associated with tumor formation and metastasis were inhibited. These included an increased incidence of apoptosis, abrogated cellular proliferation and mobility. Furthermore, Western blot analysis revealed that the function of Rabl3 was closely associated with focal adhesion kinase (FAK) phosphorylation, both in HeLa and MDA-MB-231 cell lines at the sites of Tyr 397/576/577. Our results suggested that Rabl3 may be a novel oncogene which regulates the oncological behavior of human cancer cells. As a consequence, Rabl3 can be considered as a novel candidate in the control of tumorigenesis and as a new target for anti-tumor treatment.

语种英语
所属项目编号30471581 ; 30571702 ; 2002BA711A01-09
资助者National Natural Science Foundation of China ; National High Biotechnology Development Program of China ; National Natural Science Foundation of China ; National High Biotechnology Development Program of China
WOS记录号WOS:000280050400018
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63774
专题基础医学院_北京大学人类疾病基因研究中心
作者单位1.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China
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GB/T 7714
Li, Qi,Wang, Li,Zeng, Linge,et al. Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells[J]. ONCOLOGY REPORTS,2010,24(2):433-440.
APA Li, Qi.,Wang, Li.,Zeng, Linge.,Zhang, Yingmei.,Li, Kangqi.,...&Wang, Lu.(2010).Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells.ONCOLOGY REPORTS,24(2),433-440.
MLA Li, Qi,et al."Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells".ONCOLOGY REPORTS 24.2(2010):433-440.
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