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学科主题: 药学
题名:
Molecular Analysis of Stemona Plants in China Based on Sequences of Four Chloroplast DNA Regions
作者: Fan, Lan-Lan1; Zhu, Shu2; Chen, Hu-Biao3; Yang, Dong-Hui1; Cai, Shao-Qing1; Komatsu, Katsuko2
关键词: Stemona ; molecular identification ; trnL-trnF ; petB-petD ; trnH-psbA ; trnK-rps16
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2009-08-01
卷: 32, 期:8, 页:1439-1446
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: PHYLOGENETIC-RELATIONSHIPS ; TUBEROSA ; ALKALOIDS ; EVOLUTION ; POACEAE ; GENOME
英文摘要:

Stemona sessilifolia, S. japonica and S. tuberosa are the three original sources of Stemonae Radix specified in the Chinese Pharmacopoeia (CP), and have been traditionally used for antitussive and insecticidal remedy. Significant variations in alkaloids composition and content, as well as different degrees of antitussive activities were found among them. In order to identify the genuine sources of Stemonae Radix accurately in genetic level, we determined the nucleotide sequences of chloroplast DNA trnL-trnF, trnH-psbA, petB-petD and trnK-rps16 regions of the species recorded in CP and S. parviflora, as well as the common counterfeits of Stemonae Radix, Asparagus species. The results revealed that the sequences of petB-petD and trnK-rps16 regions, showing relatively high substitution rate, were more informative than those of trnL-trnF and trnH-psbA regions. The sequences from all the four regions provided useful information to discriminate the three CP species from each other and from S. parviflora and the counterfeits. A phylogenetic tree reconstructed by the trnH-psbA sequences for 9 Stemona species distributed in China and Thailand showed that the three CP species belonged to the same clade, among which S. japonica and S. sessillifolia formed a sister group, showing closer relations to each other than to S. tuberosa. By contrast, S. parviflora was genetically far from the three CP species. Intra-species variations were observed in the three CP species. Especially, in S. tuberosa two types of petB-petD sequence and four types each of trnL-trnF, trnK-rps16 and trnH-psbA sequences resulted in 6 haplotypes; whereas, these differences had no relation with the different chemical types, but seemed to be consistent with geographical distribution.

语种: 英语
所属项目编号: 30425018
项目资助者: National Science Fund for Distinguished Young Scholars of NSFC ; Ministry of Education, Culture, Sports, Science and Technology of Japan
WOS记录号: WOS:000268545800022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63784
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 110191, Peoples R China
2.Toyama Univ, Inst Nat Med, Dept Med Resources, Div Parmacognosy, Toyama 9300194, Japan
3.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Fan, Lan-Lan,Zhu, Shu,Chen, Hu-Biao,et al. Molecular Analysis of Stemona Plants in China Based on Sequences of Four Chloroplast DNA Regions[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2009,32(8):1439-1446.
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