IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Development of a double-layer microfluidic chip with flow medium for chemotherapy resistance analysis of lung cancer
Meng, Qiang2,3; He, Zhongzhou4; Zhang, Lichuan2,5; Zhao, Long1; Li, Encheng2; Zhang, Qianqian2; Zhang, Xiaolin2; Yang, Dongxia2; Zou, Lijuan1; Gao, Zhancheng6; Wang, Qi1
关键词Chemoresistance Double-layer chip Lung cancer Microchip-based systems SPCA-1
刊名ELECTROPHORESIS
2011-11-01
DOI10.1002/elps.201100086
32期:23页:3446-3453
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TOTAL ANALYSIS SYSTEMS ; PROTEIN ; CELLS ; CULTURE ; TRANSPORTERS ; MODULATION ; INHIBITORS ; APOPTOSIS ; NETWORKS ; TRENDS
英文摘要

Integration and miniaturization are main advantages of microchip-based systems. Vertical integration of the multiple operations within a multiple-layer chip is expected to satisfy the urgent demand for high-throughput and large-scale applications. This study aimed at establishing a double-layer chip to integrate the operations including the cell culture, the identification of the protein and the detection of the cell viability onto a platform systematically and supplied with flow fresh medium continuously via a syringe pump to mimic the microenvironment in vivo. With this device, human non-small cell lung cancer cell line (SPCA-1) was cultured well; the expression and the activity of multidrug resistance-associated protein (MRP1) were detected by immunofluorescence assay for the cells pretreated with or without MK-571, a known inhibitor of MRP1; apoptosis percentages were assayed for the cells after being treated by the anticancer drug etoposide (VP-16). The results demonstrated that the function of the MRP1 was inhibited by MK-571, and the percentage of apoptotic for the cells pretreated with MK-571 was higher than that of the control (38.2 +/- 2.5% versus 12.3 +/- 0.85%, p<0.005). All these indicated that the new device could provide a suitable condition for cell culture and functional analysis in biomedical research, and MK-571 is an effective inhibitor of MRP1 associated with the viability of SPCA-1 cell line treated by VP-16.

语种英语
WOS记录号WOS:000298300900019
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63788
专题北京大学第二临床医学院
北京大学第二临床医学院_呼吸科
作者单位1.Dalian Med Univ, Grad Sch, Dalian 116023, Peoples R China
2.Dalian Med Univ, Hosp 2, Dept Resp Med, Dalian 116023, Peoples R China
3.Jining First Peoples Hosp, Dept Intens Care Unit, Jining, Peoples R China
4.Dalian Med Univ, Hosp 1, Dept Urinary Surg, Dalian 116023, Peoples R China
5.Dalian Univ, Affiliated Zhongshan Hosp, Dept Resp Med, Dalian, Peoples R China
6.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Meng, Qiang,He, Zhongzhou,Zhang, Lichuan,et al. Development of a double-layer microfluidic chip with flow medium for chemotherapy resistance analysis of lung cancer[J]. ELECTROPHORESIS,2011,32(23):3446-3453.
APA Meng, Qiang.,He, Zhongzhou.,Zhang, Lichuan.,Zhao, Long.,Li, Encheng.,...&Wang, Qi.(2011).Development of a double-layer microfluidic chip with flow medium for chemotherapy resistance analysis of lung cancer.ELECTROPHORESIS,32(23),3446-3453.
MLA Meng, Qiang,et al."Development of a double-layer microfluidic chip with flow medium for chemotherapy resistance analysis of lung cancer".ELECTROPHORESIS 32.23(2011):3446-3453.
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