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学科主题: 临床医学
题名:
Development of a double-layer microfluidic chip with flow medium for chemotherapy resistance analysis of lung cancer
作者: Meng, Qiang2,3; He, Zhongzhou4; Zhang, Lichuan2,5; Zhao, Long1; Li, Encheng2; Zhang, Qianqian2; Zhang, Xiaolin2; Yang, Dongxia2; Zou, Lijuan1; Gao, Zhancheng6; Wang, Qi1
关键词: Chemoresistance ; Double-layer chip ; Lung cancer ; Microchip-based systems ; SPCA-1
刊名: ELECTROPHORESIS
发表日期: 2011-11-01
DOI: 10.1002/elps.201100086
卷: 32, 期:23, 页:3446-3453
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: TOTAL ANALYSIS SYSTEMS ; PROTEIN ; CELLS ; CULTURE ; TRANSPORTERS ; MODULATION ; INHIBITORS ; APOPTOSIS ; NETWORKS ; TRENDS
英文摘要:

Integration and miniaturization are main advantages of microchip-based systems. Vertical integration of the multiple operations within a multiple-layer chip is expected to satisfy the urgent demand for high-throughput and large-scale applications. This study aimed at establishing a double-layer chip to integrate the operations including the cell culture, the identification of the protein and the detection of the cell viability onto a platform systematically and supplied with flow fresh medium continuously via a syringe pump to mimic the microenvironment in vivo. With this device, human non-small cell lung cancer cell line (SPCA-1) was cultured well; the expression and the activity of multidrug resistance-associated protein (MRP1) were detected by immunofluorescence assay for the cells pretreated with or without MK-571, a known inhibitor of MRP1; apoptosis percentages were assayed for the cells after being treated by the anticancer drug etoposide (VP-16). The results demonstrated that the function of the MRP1 was inhibited by MK-571, and the percentage of apoptotic for the cells pretreated with MK-571 was higher than that of the control (38.2 +/- 2.5% versus 12.3 +/- 0.85%, p<0.005). All these indicated that the new device could provide a suitable condition for cell culture and functional analysis in biomedical research, and MK-571 is an effective inhibitor of MRP1 associated with the viability of SPCA-1 cell line treated by VP-16.

语种: 英语
所属项目编号: 30470464 ; 30670532
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000298300900019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63788
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Dalian Med Univ, Grad Sch, Dalian 116023, Peoples R China
2.Dalian Med Univ, Hosp 2, Dept Resp Med, Dalian 116023, Peoples R China
3.Jining First Peoples Hosp, Dept Intens Care Unit, Jining, Peoples R China
4.Dalian Med Univ, Hosp 1, Dept Urinary Surg, Dalian 116023, Peoples R China
5.Dalian Univ, Affiliated Zhongshan Hosp, Dept Resp Med, Dalian, Peoples R China
6.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100871, Peoples R China

Recommended Citation:
Meng, Qiang,He, Zhongzhou,Zhang, Lichuan,et al. Development of a double-layer microfluidic chip with flow medium for chemotherapy resistance analysis of lung cancer[J]. ELECTROPHORESIS,2011,32(23):3446-3453.
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