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学科主题临床医学
Endothelin-1 is a potent regulator in vivo in vascular calcification and in vitro in calcification of vascular smooth muscle cells
Wu, SY; Zhang, BH; Pan, CS; Jiang, HF; Pang, YZ; Tang, CS; Qi, YF
关键词aorta endothelin-1 vitamin D calcification vascular smooth muscle cell
刊名PEPTIDES
2003-08-01
DOI10.1016/j.peptides.2003.07.008
24期:8页:1149-1156
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]VITAMIN-D-3 PLUS NICOTINE ; ARTERIAL CALCIFICATION ; RATS ; OSTEOPOROSIS ; EXPRESSION ; MECHANISM ; PROTEIN ; DISEASE ; LINK ; BONE
英文摘要

We observed changes of endothelin content and endothelin mRNA in vivo in vascular calcification and in vitro in calcification of vascular smooth muscle cells to explore the role of endothelin in vascular calcification. Calcification model in vivo was induced by administration of Vitamin D-3 plus nicotine. Calcification of vascular smooth muscle cells (VSMCs) was induced by beta-glycerophosphate. Endothelin content was measured by using radioimmunoassay. Endothelin mRNA amount was determined by using competitive quantitative RT-PCR. The results showed that calcium content, Ca-45(2+) uptake and alkaline phosphatase (ALP) activity were increased in calcified VSMCs, compared with controls, but were decreased, compared with calcified VSMCs plus BQ123 group. The endothelin content in the medium and endothelin mRNA in VSMCs were elevated by 35 and 120% (P < 0.05), respectively, compared with those normal VSMCs. Calcium content, Ca-45(2+) accumulation and ALP activity in calcified arteries increased by 5.0-, 1.4-, and 1.4-fold. The endothelin levels in plasma and aorta as well as the amount of endothelin mRNA in calcified aorta were increased by 102, 103, and 22%, respectively, compared with control group. However, calcium content, Ca-45(2+) uptake and ALP activity in VDN plus bosentan group was 33, 36.7, and 40.4% lower than those in VDN group. These results indicated an upregulated endothelin gene expression as well as an increased production of endothelin in calcified aorta and VSMCs with BQ123 and bosentan significantly reducing vascular calcification. This suggested that endothelin might be involved in pathogenesis of vascular calcification. (C) 2003 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000186823300007
引用统计
被引频次:39[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63817
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Inst Cardiovasc Dis, Hosp 1, Beijing 100034, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Wu, SY,Zhang, BH,Pan, CS,et al. Endothelin-1 is a potent regulator in vivo in vascular calcification and in vitro in calcification of vascular smooth muscle cells[J]. PEPTIDES,2003,24(8):1149-1156.
APA Wu, SY.,Zhang, BH.,Pan, CS.,Jiang, HF.,Pang, YZ.,...&Qi, YF.(2003).Endothelin-1 is a potent regulator in vivo in vascular calcification and in vitro in calcification of vascular smooth muscle cells.PEPTIDES,24(8),1149-1156.
MLA Wu, SY,et al."Endothelin-1 is a potent regulator in vivo in vascular calcification and in vitro in calcification of vascular smooth muscle cells".PEPTIDES 24.8(2003):1149-1156.
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