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学科主题: 临床医学
题名:
Comparative Study of the Biological Characteristics of Mesenchymal Stem Cells from Bone Marrow and Peripheral Blood of Rats
作者: Fu, Wei-Li1,2; Zhang, Ji-Ying1; Fu, Xin1; Duan, Xiao-Ning1; Leung, Kevin Kar Ming1; Jia, Zhu-Qing3; Wang, Wei-Ping3; Zhou, Chun-Yan3; Yu, Jia-Kuo1
刊名: TISSUE ENGINEERING PART A
发表日期: 2012-09-01
DOI: 10.1089/ten.tea.2011.0530
卷: 18, 期:17-18, 页:1793-1803
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology
关键词[WOS]: COLONY-STIMULATING FACTOR ; HEMATOPOIETIC-STEM ; PROGENITOR CELLS ; STROMAL CELLS ; ADIPOSE-TISSUE ; UMBILICAL-CORD ; IN-VIVO ; MOBILIZATION ; DIFFERENTIATION ; THERAPY
英文摘要:

Mesenchymal stem cells (MSCs) from adult exhibit self-renewal and multilineage differentiation capacities, making the MSCs promising candidates for cell therapy and tissue engineering. Although bone marrow (BM) is the most universal source of MSCs, other tissues may also contain MSCs. Peripheral blood (PB), in particular, arises as the most attractive source of MSCs due to easy accessibility and noninvasive procedure. However, it is not certain that PB-MSCs have the equal biological characteristics to those of BM-MSCs. The purpose of this study was to compare the biological characteristics between BM-MSCs and PB-MSCs. We adopted granulocyte colony-stimulating factor combined with CXCR4 antagonist AMD3100 to stimulate MSCs to release into blood circulation of the rats. PB-MSCs were obtained from mobilized PB and expanded in long-term culture. BM-MSCs were isolated from the femur and tibia medullary canal of the same rats by density gradient centrifugation. After cell expansion in vitro, cell surface markers and multipotentiality analysis were performed to identify MSCs. Apoptosis resistance to H2O2-induced apoptosis, proliferation kinetics, cellular senescence, and karyotype analysis were measured to compare the biological characteristics of PB-MSCs and BM-MSCs. PB-MSCs with the typical adherent fibroblast-like morphology were similar to that of BM-MSCs. Both PB-MSCs and BM-MSCs were positive for CD44 and CD90, and negative for CD34 and CD45. They both exhibited trilineage differentiation potential and expressed lineage-specific genes. Although the BM-MSCs showed stronger osteogenic and adipogenic differentiation, PB-MSCs displayed a more chondrogenic capacity. Further, BM-MSCs have greater proliferation ability. Apoptosis resistance and cellular senescence were similar in MSCs derived from both sources. The results of our study demonstrate that PB-MSCs have similar biological characteristics to those of BM-MSCs despite certain minor differences, suggesting PB as a possible alternative source for MSCs.

语种: 英语
所属项目编号: 30471783 ; 20030001009 ; 7052046
项目资助者: National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation
WOS记录号: WOS:000308704600006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63836
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ Third Hosp, Inst Sports Med, Beijing 100191, Peoples R China
2.Sichuan Univ, W China Hosp, Dept Orthoped Surg, Chengdu 610064, Peoples R China
3.Peking Univ Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China

Recommended Citation:
Fu, Wei-Li,Zhang, Ji-Ying,Fu, Xin,et al. Comparative Study of the Biological Characteristics of Mesenchymal Stem Cells from Bone Marrow and Peripheral Blood of Rats[J]. TISSUE ENGINEERING PART A,2012,18(17-18):1793-1803.
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