IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Comparative Study of the Biological Characteristics of Mesenchymal Stem Cells from Bone Marrow and Peripheral Blood of Rats
Fu, Wei-Li1,2; Zhang, Ji-Ying1; Fu, Xin1; Duan, Xiao-Ning1; Leung, Kevin Kar Ming1; Jia, Zhu-Qing3; Wang, Wei-Ping3; Zhou, Chun-Yan3; Yu, Jia-Kuo1
刊名TISSUE ENGINEERING PART A
2012-09-01
DOI10.1089/ten.tea.2011.0530
18期:17-18页:1793-1803
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology
资助者National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology
关键词[WOS]COLONY-STIMULATING FACTOR ; HEMATOPOIETIC-STEM ; PROGENITOR CELLS ; STROMAL CELLS ; ADIPOSE-TISSUE ; UMBILICAL-CORD ; IN-VIVO ; MOBILIZATION ; DIFFERENTIATION ; THERAPY
英文摘要

Mesenchymal stem cells (MSCs) from adult exhibit self-renewal and multilineage differentiation capacities, making the MSCs promising candidates for cell therapy and tissue engineering. Although bone marrow (BM) is the most universal source of MSCs, other tissues may also contain MSCs. Peripheral blood (PB), in particular, arises as the most attractive source of MSCs due to easy accessibility and noninvasive procedure. However, it is not certain that PB-MSCs have the equal biological characteristics to those of BM-MSCs. The purpose of this study was to compare the biological characteristics between BM-MSCs and PB-MSCs. We adopted granulocyte colony-stimulating factor combined with CXCR4 antagonist AMD3100 to stimulate MSCs to release into blood circulation of the rats. PB-MSCs were obtained from mobilized PB and expanded in long-term culture. BM-MSCs were isolated from the femur and tibia medullary canal of the same rats by density gradient centrifugation. After cell expansion in vitro, cell surface markers and multipotentiality analysis were performed to identify MSCs. Apoptosis resistance to H2O2-induced apoptosis, proliferation kinetics, cellular senescence, and karyotype analysis were measured to compare the biological characteristics of PB-MSCs and BM-MSCs. PB-MSCs with the typical adherent fibroblast-like morphology were similar to that of BM-MSCs. Both PB-MSCs and BM-MSCs were positive for CD44 and CD90, and negative for CD34 and CD45. They both exhibited trilineage differentiation potential and expressed lineage-specific genes. Although the BM-MSCs showed stronger osteogenic and adipogenic differentiation, PB-MSCs displayed a more chondrogenic capacity. Further, BM-MSCs have greater proliferation ability. Apoptosis resistance and cellular senescence were similar in MSCs derived from both sources. The results of our study demonstrate that PB-MSCs have similar biological characteristics to those of BM-MSCs despite certain minor differences, suggesting PB as a possible alternative source for MSCs.

语种英语
所属项目编号30471783 ; 20030001009 ; 7052046
资助者National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Key Doctoral Foundation of Chinese Education Commission ; Beijing Natural Science Foundation
WOS记录号WOS:000308704600006
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63836
专题北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ Third Hosp, Inst Sports Med, Beijing 100191, Peoples R China
2.Sichuan Univ, W China Hosp, Dept Orthoped Surg, Chengdu 610064, Peoples R China
3.Peking Univ Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Fu, Wei-Li,Zhang, Ji-Ying,Fu, Xin,et al. Comparative Study of the Biological Characteristics of Mesenchymal Stem Cells from Bone Marrow and Peripheral Blood of Rats[J]. TISSUE ENGINEERING PART A,2012,18(17-18):1793-1803.
APA Fu, Wei-Li.,Zhang, Ji-Ying.,Fu, Xin.,Duan, Xiao-Ning.,Leung, Kevin Kar Ming.,...&Yu, Jia-Kuo.(2012).Comparative Study of the Biological Characteristics of Mesenchymal Stem Cells from Bone Marrow and Peripheral Blood of Rats.TISSUE ENGINEERING PART A,18(17-18),1793-1803.
MLA Fu, Wei-Li,et al."Comparative Study of the Biological Characteristics of Mesenchymal Stem Cells from Bone Marrow and Peripheral Blood of Rats".TISSUE ENGINEERING PART A 18.17-18(2012):1793-1803.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Fu, Wei-Li]的文章
[Zhang, Ji-Ying]的文章
[Fu, Xin]的文章
百度学术
百度学术中相似的文章
[Fu, Wei-Li]的文章
[Zhang, Ji-Ying]的文章
[Fu, Xin]的文章
必应学术
必应学术中相似的文章
[Fu, Wei-Li]的文章
[Zhang, Ji-Ying]的文章
[Fu, Xin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。