IR@PKUHSC  > 北京大学药学院
学科主题药学
Transferrin receptor specific nanocarriers conjugated with functional 7peptide for oral drug delivery
Du, Wenwen; Fan, Yuchen; Zheng, Nan; He, Bing; Yuan, Lan; Zhang, Hua; Wang, Xueqing; Wang, Jiancheng; Zhang, Xuan; Zhang, Qiang
关键词Transferrin receptor specific Functional 7peptide Functional nanocarriers Caco-2 cells Oral delivery
刊名BIOMATERIALS
2013
DOI10.1016/j.biomaterials.2012.10.003
34期:3页:794-806
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
资助者National Research Fund for Fundamental Key Project ; National Natural Science Foundation of China ; National Research Fund for Fundamental Key Project ; National Natural Science Foundation of China
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]POORLY SOLUBLE DRUGS ; POLYMERIC MICELLES ; ANTICANCER DRUGS ; EPITHELIAL-CELLS ; MEDIATED ENDOCYTOSIS ; TARGETED DELIVERY ; CELLULAR UPTAKE ; NANOPARTICLES ; TRANSPORT ; CLATHRIN
英文摘要

In an attempt to increase the interaction of a nanocarrier system with gastrointestinal epithelial cells, a transferrin receptor (TfR) specific 7peptide was conjugated to PEG-b-PCL copolymer and the functional nanocarriers were constructed and characterized. The endocytosis, intracellular trafficking and transcytosis of such nanocarriers loaded with coumarin 6 (7pep-M-C6) in a human colon carcinoma cell line (Caco-2) were investigated, followed by the in vivo intestine distribution study. The real-time imaging of live cell, three dimensional reconstruction of confocal image, quantitative colocalization analysis and other techniques were applied. First, the TfR expression was confirmed in Caco-2. Then, 7pep-M-C6 exhibited higher intracellular uptake compared with unmodified nanocarriers. In a live cell study, 7pep-M-C6 demonstrated faster uptake kinetics especially in the surface of cells. Together with a competition study using TfR antibody, it was proved that the increased cellular uptake was due to a receptor-mediated mechanism. Besides the unspecific endocytosis pathway, 7pep-M-C6 was found to enter the cells through a specific clathrin-mediated mechanism, related to the expression of TfR on Caco-2 cells. Possibly for this reason, 7pep-M-C6 tended to colocalize more with late endosomes and lysosomes than the control micelles. Also for the same mechanism, the increased transport of 7pep-M-C6 across Caco-2 monolayer was found, through a transcellular but not a paracellular pathway, while an increased in vivo intestinal distribution of 7pep-M-C6 was observed. In conclusion, the functional nanocarriers could specifically interact with gastrointestinal endothelial cells, increase their transport and alter their pathway as a result. (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号2009CB930300 ; 81130059
资助者National Research Fund for Fundamental Key Project ; National Natural Science Foundation of China ; National Research Fund for Fundamental Key Project ; National Natural Science Foundation of China
WOS记录号WOS:000312759800020
引用统计
被引频次:66[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63850
专题北京大学药学院
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Du, Wenwen,Fan, Yuchen,Zheng, Nan,et al. Transferrin receptor specific nanocarriers conjugated with functional 7peptide for oral drug delivery[J]. BIOMATERIALS,2013,34(3):794-806.
APA Du, Wenwen.,Fan, Yuchen.,Zheng, Nan.,He, Bing.,Yuan, Lan.,...&Zhang, Qiang.(2013).Transferrin receptor specific nanocarriers conjugated with functional 7peptide for oral drug delivery.BIOMATERIALS,34(3),794-806.
MLA Du, Wenwen,et al."Transferrin receptor specific nanocarriers conjugated with functional 7peptide for oral drug delivery".BIOMATERIALS 34.3(2013):794-806.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Du, Wenwen]的文章
[Fan, Yuchen]的文章
[Zheng, Nan]的文章
百度学术
百度学术中相似的文章
[Du, Wenwen]的文章
[Fan, Yuchen]的文章
[Zheng, Nan]的文章
必应学术
必应学术中相似的文章
[Du, Wenwen]的文章
[Fan, Yuchen]的文章
[Zheng, Nan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。