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Effects of timely insulin treatment on protection of beta cells in a rat model of type 2 diabetes mellitus
Zhou, YS; Gao, Y; Guo, XH; Li, B; Shu, W; Chi, JM
关键词insulin type 2 diabetes rat model beta cells
刊名CHINESE MEDICAL JOURNAL
2004-10-01
117期:10页:1523-1529
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]ANIMAL-MODEL ; GROWTH ; GLUCOSE ; NIDDM ; ISLET ; DIFFERENTIATION ; HYPERGLYCEMIA
英文摘要

Background Insulin treatment plays a key role in management of diabetes mellitus. Clinical researches showed that extra improvements in restoration of insulin secretion of pancreatic beta cells were found in patients with newly diagnosed type 2 diabetes. The purpose of this study was to investigate the effects of early insulin treatment on insulin mRNA expression and morphological alterations of cells in a Sprague Dawley (SD) rat model of type 2 diabetes.

Methods A rat model of type 2 diabetes mellitus ( T2DM) was induced by a high fat diet (high energy, HE) and low doses of streptozotoxin ( STZ, 40 mg/kg). A group of diabetic rats was then injected with protamine zinc insulin (PZI, 1 -2 U . kg(-1) . d(-1)) for one week. Insulin mRNA expression, morphological features of pancreatic islets, and metabolic parameters were examined in rats using reverse transcriptase-polymerase chain reaction ( RT-PCR immunohistochemistry, and other techniques.

Results In insulin-treated diabetic rats, insulin mRNA levels prominently increased by 81.3% ( P < 0.05), as compared with untreated diabetic rats. Moreover, timely insulin treatment noticeably improved the insulin content of beta cells, with an increase of 10.2% ( P < 0.05 despite a slight reduction in fasting blood glucose ( FBG), triglyceride (TG), and free fatty acid (FFA) levels, as compared to an untreated diabetic group.

Conclusion Insulin treatment at the onset of T2DM effectively improves insulin synthesis, as confirmed by morphological changes to beta cells in a rat model of type 2 diabetes.

语种英语
WOS记录号WOS:000224787500016
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63855
专题北京大学第一临床医学院_内分泌内科
作者单位1.Beijing Hosp, Dept Plast Surg, Beijing 100730, Peoples R China
2.Beijing Inst Geriatr, Dept Biochem, Beijing 100730, Peoples R China
3.Beijing Hosp, Dept Endocrinol, Beijing 100730, Peoples R China
4.Peking Univ, Hosp 1, Dept Endocrinol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhou, YS,Gao, Y,Guo, XH,et al. Effects of timely insulin treatment on protection of beta cells in a rat model of type 2 diabetes mellitus[J]. CHINESE MEDICAL JOURNAL,2004,117(10):1523-1529.
APA Zhou, YS,Gao, Y,Guo, XH,Li, B,Shu, W,&Chi, JM.(2004).Effects of timely insulin treatment on protection of beta cells in a rat model of type 2 diabetes mellitus.CHINESE MEDICAL JOURNAL,117(10),1523-1529.
MLA Zhou, YS,et al."Effects of timely insulin treatment on protection of beta cells in a rat model of type 2 diabetes mellitus".CHINESE MEDICAL JOURNAL 117.10(2004):1523-1529.
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