|Cytokine-induced killer cells showing multidrug resistance and remaining cytotoxic activity to tumor cells after transfected with mdr1 cDNA|
|Li, HF; Yang, YH; Shi, YJ; Wang, YQ; Zhu, P|
|关键词||genes, mdr killer cells lymphokine activated leukemia immunotherpy adoptive|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||IMMUNOLOGICAL EFFECTOR-CELLS ; SEVERE COMBINED IMMUNODEFICIENCY ; CHRONIC MYELOID-LEUKEMIA ; T-CELLS ; INFILTRATING LYMPHOCYTES ; ANTITUMOR-ACTIVITY ; GENE-TRANSFER ; IN-VITRO ; IMMUNOTHERAPY ; CANCER|
Background Routine treatment of cancer such as surgery, radiation or chemotherapy is sometimes unable to erdiacate metastatic malignant cells. So we tried a new method and increased the adoptive immunotherapy of Cytokine-induced killer (CIK) cells in tumor patients and the multidrug resistance (mdr1) cDNA was transfected into CIK cells.
Methods CIK cells were obtained from peripheral blood and induced by IFN-gamma, anti-CD3 monoclonal antibody, IL-2 and IL-1. CIK cells were transfected with plasmid PHaMDR containing human mdr1 cDNA by electroporation. RT-PCR was used to detect mdr1 mRNA in transfected CIK cells. P-glycoprotein (P-gp) expressed on surface of CIK cells was assayed by FITC-conjugated anti-P-gp monoclonal antibody and flow cytometry. Multidrug resistance to doxorubicin and colchicine and cytotoxic activity to human breast cancer cell line MCF7 were performed using MTT method.
Results mdr1 mRNA was detected in transfected CIK cells. P-gp was expressed on the surface of the transfected CIK cells, and the P-gp positive cells reached 21% - 37% of the total CIK cells after transfection. The IC50 to doxorubicin increased to 22.3 - 45.8 times, and that to colchicines to 6.7 - 11.35 times, as compared to those of untransfected CIK cells. However, the cytotoxic activity to MCF7 cell line remained unaltered.
Conclusions CIK cells were successfully transfected with mdr1 cDNA by using electroporation. The transfected CIK cells had the characteristics of multidrug resistance without change in their cytotoxic activity to tumor cells.
|作者单位||Peking Univ, Hosp 1, Dept Hematol, Beijing 100034, Peoples R China|
|Li, HF,Yang, YH,Shi, YJ,et al. Cytokine-induced killer cells showing multidrug resistance and remaining cytotoxic activity to tumor cells after transfected with mdr1 cDNA[J]. CHINESE MEDICAL JOURNAL,2004,117(9):1348-1352.|
|APA||Li, HF,Yang, YH,Shi, YJ,Wang, YQ,&Zhu, P.(2004).Cytokine-induced killer cells showing multidrug resistance and remaining cytotoxic activity to tumor cells after transfected with mdr1 cDNA.CHINESE MEDICAL JOURNAL,117(9),1348-1352.|
|MLA||Li, HF,et al."Cytokine-induced killer cells showing multidrug resistance and remaining cytotoxic activity to tumor cells after transfected with mdr1 cDNA".CHINESE MEDICAL JOURNAL 117.9(2004):1348-1352.|