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学科主题临床医学
Vitamin D-binding protein (group-specific component) has decreased expression in rheumatoid arthritis
Yan, X.1; Zhao, Y.2; Pan, J.2; Fang, K.2; Wang, Y.2; Li, Z.3; Chang, X.2,4
关键词rheumatoid arthritis vitamin D-binding protein (VDBP) / group-specific component (Gc) 1,25-dihydroxyvitamin D3 ankylosing spondylitis SNP
刊名CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
2012-07-01
30期:4页:525-533
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
研究领域[WOS]Rheumatology
关键词[WOS]GENOME-WIDE ASSOCIATION ; MASS-SPECTROMETRY ; SYNOVIAL-FLUID ; D METABOLITES ; GC ; BIOMARKERS ; GLOBULIN ; THERAPY ; SERUM
英文摘要

Objective

This study used a proteomic approach to screen the proteins with decreased expression in the synovial tissues of rheumatoid arthritis (RA) patients by comparing their expression profiles to that of osteoarthritis (OA) and ankylosing spondylitis (AS) patients. The result was complemented by a SNP analysis.

Methods

Proteins extracted from the synovial membranes (n=10 for each disease) were separated by 2-D electrophoresis. The proteins with significantly decreased expression in the RA samples were subjected to MALD1-TOFITOF MS. The result was verified using western blotting. Mg SNPs located in the targeted gene were assessed using the Tayman assay in a cohort of 267 Chinese patients with RA, 51 patients with AS and 160 healthy controls. The genotyping result was confirmed in a large cohort of 389 patients with RA, 200 patients with AS and 371 healthy controls

Results

The proteomic approach detected significantly decreased expression of vitamin D-binding protein (VDBP) in the synovial membranes from patients with RA, which was confirmed with western blot analysis. rs2282679 was significantly associated with RA and AS (P=0.026794 and 0.007566, respectively). The result was confirmed in a large cohort of RA (OR=0.678639, 95%C1=10.541113-0.851118.1, p=0.000776) and AS (OR=0.564053, 95%C1=10.433716-0.7335581, p=.1.79e-005).

Conclusion

1,25-dihydroxyvitamin D3 inhibits cell proliferation. immunoglobulin production and the release of c)′tokines through binding to VDBP. VDBP also mediates bone resorption by activating osteoclasts. The decreased expression and the genetic effect of VDBP in RA suggest a novel pathogenic pathway that vitamin D contributes to the arthritic process of the disease.

语种英语
WOS记录号WOS:000308897200012
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63881
专题北京大学第二临床医学院_风湿免疫科
作者单位1.Shandong Univ, Shandong Qianfoshan Hosp, Orthopaed Surg Ctr, Jinan 250100, Shandong, Peoples R China
2.Shandong Acad Med Sci, Med Biotechnol Res Ctr, Prov Lab Modern Med & Technol Shandong, Natl Lab Biodrugs,Minist Hlth, Jinan, Shandong, Peoples R China
3.Beijing Univ, Dept Rheumatol, Peoples Hosp, Beijing 100871, Peoples R China
4.Shandong Univ, Lab Res Ctr, Shandong Qianfoshan Hosp, Jinan, Shandong, Peoples R China
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GB/T 7714
Yan, X.,Zhao, Y.,Pan, J.,et al. Vitamin D-binding protein (group-specific component) has decreased expression in rheumatoid arthritis[J]. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY,2012,30(4):525-533.
APA Yan, X..,Zhao, Y..,Pan, J..,Fang, K..,Wang, Y..,...&Chang, X..(2012).Vitamin D-binding protein (group-specific component) has decreased expression in rheumatoid arthritis.CLINICAL AND EXPERIMENTAL RHEUMATOLOGY,30(4),525-533.
MLA Yan, X.,et al."Vitamin D-binding protein (group-specific component) has decreased expression in rheumatoid arthritis".CLINICAL AND EXPERIMENTAL RHEUMATOLOGY 30.4(2012):525-533.
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