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学科主题: 临床医学
题名:
Vitamin D-binding protein (group-specific component) has decreased expression in rheumatoid arthritis
作者: Yan, X.1; Zhao, Y.2; Pan, J.2; Fang, K.2; Wang, Y.2; Li, Z.3; Chang, X.2,4
关键词: rheumatoid arthritis ; vitamin D-binding protein (VDBP) / group-specific component (Gc) ; 1,25-dihydroxyvitamin D3 ; ankylosing spondylitis ; SNP
刊名: CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
发表日期: 2012-07-01
卷: 30, 期:4, 页:525-533
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: GENOME-WIDE ASSOCIATION ; MASS-SPECTROMETRY ; SYNOVIAL-FLUID ; D METABOLITES ; GC ; BIOMARKERS ; GLOBULIN ; THERAPY ; SERUM
英文摘要:

Objective

This study used a proteomic approach to screen the proteins with decreased expression in the synovial tissues of rheumatoid arthritis (RA) patients by comparing their expression profiles to that of osteoarthritis (OA) and ankylosing spondylitis (AS) patients. The result was complemented by a SNP analysis.

Methods

Proteins extracted from the synovial membranes (n=10 for each disease) were separated by 2-D electrophoresis. The proteins with significantly decreased expression in the RA samples were subjected to MALD1-TOFITOF MS. The result was verified using western blotting. Mg SNPs located in the targeted gene were assessed using the Tayman assay in a cohort of 267 Chinese patients with RA, 51 patients with AS and 160 healthy controls. The genotyping result was confirmed in a large cohort of 389 patients with RA, 200 patients with AS and 371 healthy controls

Results

The proteomic approach detected significantly decreased expression of vitamin D-binding protein (VDBP) in the synovial membranes from patients with RA, which was confirmed with western blot analysis. rs2282679 was significantly associated with RA and AS (P=0.026794 and 0.007566, respectively). The result was confirmed in a large cohort of RA (OR=0.678639, 95%C1=10.541113-0.851118.1, p=0.000776) and AS (OR=0.564053, 95%C1=10.433716-0.7335581, p=.1.79e-005).

Conclusion

1,25-dihydroxyvitamin D3 inhibits cell proliferation. immunoglobulin production and the release of c)′tokines through binding to VDBP. VDBP also mediates bone resorption by activating osteoclasts. The decreased expression and the genetic effect of VDBP in RA suggest a novel pathogenic pathway that vitamin D contributes to the arthritic process of the disease.

语种: 英语
WOS记录号: WOS:000308897200012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63881
Appears in Collections:北京大学第二临床医学院_风湿免疫科_期刊论文

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作者单位: 1.Shandong Univ, Shandong Qianfoshan Hosp, Orthopaed Surg Ctr, Jinan 250100, Shandong, Peoples R China
2.Shandong Acad Med Sci, Med Biotechnol Res Ctr, Prov Lab Modern Med & Technol Shandong, Natl Lab Biodrugs,Minist Hlth, Jinan, Shandong, Peoples R China
3.Beijing Univ, Dept Rheumatol, Peoples Hosp, Beijing 100871, Peoples R China
4.Shandong Univ, Lab Res Ctr, Shandong Qianfoshan Hosp, Jinan, Shandong, Peoples R China

Recommended Citation:
Yan, X.,Zhao, Y.,Pan, J.,et al. Vitamin D-binding protein (group-specific component) has decreased expression in rheumatoid arthritis[J]. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY,2012,30(4):525-533.
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