IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Comparison of Osteogenic Potentials of BMP4 Transduced Stem Cells from Autologous Bone Marrow and Fat Tissue in a Rabbit Model of Calvarial Defects
Lin, Lin1; Shen, Qi2; Wei, Xuelei3; Hou, Yu1; Xue, Tao1; Fu, Xin1; Duan, Xiaoning1; Yu, Changlong1
关键词Bone marrow stem cells Adipose-derived stem cells Bone morphogenetic protein 4 Gene therapy Tissue engineering
刊名CALCIFIED TISSUE INTERNATIONAL
2009-07-01
DOI10.1007/s00223-009-9250-x
85期:1页:55-65
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism
资助者State Sports General Administration of P. R. China ; State Sports General Administration of P. R. China
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]MUSCLE-DERIVED CELLS ; STROMAL CELLS ; ADIPOSE-TISSUE ; MORPHOGENETIC PROTEIN-4 ; EXPRESSING BMP4 ; GENE-THERAPY ; IN-VITRO ; REPAIR ; REGENERATION ; RAT
英文摘要

We compared bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) of adult rabbits under identical conditions in terms of their culture characteristics, proliferation capacity, osteogenic differentiation potentials induced by adenovirus-containing bone morphogenetic protein 4 (Ad-BMP4) in vitro, and capacity to repair calvarial defects in the rabbit model by autologous transplantation ex vivo. According to the results of growth curve, cell cycle, and telomerase activity analysis, ADSCs possess a higher proliferation potential. Both of the Ad-BMP4 transduced MSCs expressed BMP4 mRNA and protein and underwent osteogenic differentiation. Up-regulated mRNA expression of all osteogenic genes was observed in differentiated BMSCs and ADSCs, but with different patterns confirmed by real-time RT-PCR. Deposition of calcified extracellular matrix was significantly greater in differentiated ADSCs compared with differentiated BMSCs. X-ray and histological examination indicated significant bone regeneration in the calvarial defects transplanted with Ad-BMP4 transduced autologous MSCs compared to the control groups. There was no significant difference in new bone formation in Ad-BMP4 transduced MSCs based on quantitative digital analysis of histological sections. The use of ADSCs often resulted in the growth of fat tissue structures in the control groups, and the fat tissue structures were not seen with BMSC cells. Our data demonstrate that BMP4 can be potently osteoinductive in vivo, resulting in bone repair. ADSCs may be an attractive alternative to BMSCs for bone tissue engineering under appropriate stimuli. But the easy adipogenic differentiation needs to be considered when choosing adipose tissue for specific clinical application.

语种英语
资助者State Sports General Administration of P. R. China ; State Sports General Administration of P. R. China
WOS记录号WOS:000268002200008
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63884
Collection北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ Third Hosp, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ First Hosp, Inst Urol, Beijing 100034, Peoples R China
3.Tianjin Hosp, Dept Orthopaed Surg, Tianjin 300211, Peoples R China
Recommended Citation
GB/T 7714
Lin, Lin,Shen, Qi,Wei, Xuelei,et al. Comparison of Osteogenic Potentials of BMP4 Transduced Stem Cells from Autologous Bone Marrow and Fat Tissue in a Rabbit Model of Calvarial Defects[J]. CALCIFIED TISSUE INTERNATIONAL,2009,85(1):55-65.
APA Lin, Lin.,Shen, Qi.,Wei, Xuelei.,Hou, Yu.,Xue, Tao.,...&Yu, Changlong.(2009).Comparison of Osteogenic Potentials of BMP4 Transduced Stem Cells from Autologous Bone Marrow and Fat Tissue in a Rabbit Model of Calvarial Defects.CALCIFIED TISSUE INTERNATIONAL,85(1),55-65.
MLA Lin, Lin,et al."Comparison of Osteogenic Potentials of BMP4 Transduced Stem Cells from Autologous Bone Marrow and Fat Tissue in a Rabbit Model of Calvarial Defects".CALCIFIED TISSUE INTERNATIONAL 85.1(2009):55-65.
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