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学科主题: 药学
题名:
Redox-responsive catiomer based on PEG-ss-chitosan oligosaccharide-ss-polyethylenimine copolymer for effective gene delivery
作者: Jia, Lejiao1; Li, Zhenyu2; Zhang, Dianrui1; Zhang, Qiang3; Shen, Jingyi1; Guo, Hejian1; Tian, Xiaona1; Liu, Guangpu1; Zheng, Dandan1; Qi, Lisi1
刊名: POLYMER CHEMISTRY
发表日期: 2013
DOI: 10.1039/c2py20781h
卷: 4, 期:1, 页:156-165
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Polymer Science
研究领域[WOS]: Polymer Science
关键词[WOS]: IN-VITRO ; TRIBLOCK COPOLYMERS ; DNA POLYPLEXES ; PLASMID DNA ; TRANSFECTION EFFICIENCY ; NONVIRAL VECTORS ; LOW CYTOTOXICITY ; CANCER-THERAPY ; NANOPARTICLES ; PEGYLATION
英文摘要:

The chitosan oligosaccharide-based disulfide-containing polyethylenimine derivative PEG-ss-COS-ss-PEI was synthesized and evaluated as a nonviral gene delivery carrier. The structure of the obtained polymers was confirmed by H-1 NMR and FTIR. PEG-ss-COS-ss-PEI copolymers could effectively condense DNA into small particles with average diameters less than 120 nm and the zeta potential of +15.7 mV at the N/P ratio of 15/1. Additionally, the resultant polyplexes showed excellent colloidal stability against 150 mM NaCl and had a better buffering capacity of similar to 44%, which was more than double the buffering capacity of PEI1.8k (similar to 20%). In the presence of 10 mM glutathione (GSH), however, polyplexes of PEG-ss-COS-ss-PEI were rapidly unpacked, as revealed by significant increase of particle sizes to over 800 nm. In vitro experiments revealed that the PEG-ss-COS-ss-PEI copolymers not only had much lower cytotoxicity, but also displayed high transfection efficiency as compared to the control branch 25 kDa PEI. This study indicates that a reducibly degradable copolymer PEG-ss-COS-ss-PEI composed of low molecular weight PEI, chitosan oligosaccharide and PEG via disulfide-containing linkages can be a promising gene delivery carrier.

语种: 英语
所属项目编号: 2009CB930300
项目资助者: National Basic Research Program of China (973 Program)
WOS记录号: WOS:000311970900017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63895
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Shandong Univ, Dept Pharmaceut, Coll Pharm, Jinan 250012, Peoples R China
2.Shandong Univ, Key Lab Chem Biol, Minist Educ, Coll Pharm, Jinan 250012, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Jia, Lejiao,Li, Zhenyu,Zhang, Dianrui,et al. Redox-responsive catiomer based on PEG-ss-chitosan oligosaccharide-ss-polyethylenimine copolymer for effective gene delivery[J]. POLYMER CHEMISTRY,2013,4(1):156-165.
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