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学科主题: 临床医学
题名:
Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes
作者: Fan, Yong1; Zhao, Hong-Cui2,3,4; Liu, Jianqiao1; Tan, Tao5,6; Ding, Ting2,3; Li, Rong2,3,4; Zhao, Yue2,4; Yan, Jie2,3; Sun, Xiaofang1; Yu, Yang2,3,4; Qiao, Jie2,3,4
刊名: SCIENTIFIC REPORTS
发表日期: 2015-02-03
DOI: 10.1038/srep08192
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: EPIDERMAL-GROWTH-FACTOR ; SINGLE-CELL ; KINASE 1 ; CYTOKINESIS FAILURE ; NEUROTROPHIC FACTOR ; EMBRYO DEVELOPMENT ; DYNACTIN COMPLEX ; GENE-EXPRESSION ; STEM-CELLS ; ANEUPLOIDY
英文摘要:

Fertilisation is the first step in embryonic development, and dynamic changes of key genes may potentially improve assisted reproduction techniques efficiency during this process. Here, we analysed genes that were differentially expressed between oocytes and zygotes and focused on cytokinesis- related genes. Plk1 and Dctn3 were identified as showing dramatic changes in expression during fertilisation and were suggested to play a key role in inducing aneuploidy in zygotes and 8-cell embryos. Moreover, we found that maternal Plk1 and Dctn3 were expressed at lower levels in in vitro matured oocytes, which may have contributed to the high ratio of resulting embryos with abnormal Plk1 and Dctn3 expression levels, thereby reducing the developmental competence of the resulting embryos. Furthermore, the overexpression of Dctn3 can silence Plk1 expression, which suggests a potential regulation mechanism. In conclusion, our present study showed that aberrant expression of Plk1 and Dctn3 increases embryo aneuploidy and developmental failure, particularly in in vitro matured oocytes. Our results facilitate a better understanding of the effects of oocyte maternal gene expression on embryonic development and can be used to improve the outcome of assisted reproduction techniques.

语种: 英语
所属项目编号: 2014CB943203 ; 2011CB944504 ; 31371521 ; 81100473 ; 81370766 ; 2012J2200006 ; Yq2013135 ; 2013KJCX0149
项目资助者: Ministry of Science and Technology of China ; National Natural Science Funds ; Beijing Nova Program ; Zhujiang Science and Technology Star Project of Guangzhou ; Guangdong Province Higher Education Funding
WOS记录号: WOS:000348703200003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63911
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Yunnan Key Lab Primate Biomed Res, Kunming 650500, Peoples R China
3.Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Major Obstetr Dis Guangdong Prov, Guangzhou 510150, Guangdong, Peoples R China
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
5.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
6.Kunming Biomed Int & Natl Engn Res Ctr Biomed & A, Kunming 650500, Peoples R China

Recommended Citation:
Fan, Yong,Zhao, Hong-Cui,Liu, Jianqiao,et al. Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes[J]. SCIENTIFIC REPORTS,2015,5.
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