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Honokiol up-regulates prostacyclin synthease protein expression an inhibits endothelial cell apoptosis
Zhang, Xiaoxue; Chen, Shizhong; Wang, Yinye
关键词honokiol thrombosis rat aortic endothelial cells prostacyclin (PGI(2)) prostacyclin synthease apoptosis
刊名EUROPEAN JOURNAL OF PHARMACOLOGY
2007-01-05
DOI10.1016/j.ejphar.2006.09.065
554期:1页:1-7
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]MAGNOLIA-OFFICINALIS ; IN-VITRO ; ACTIVATED PLATELETS ; LIPID-PEROXIDATION ; INJURY ; CYCLOOXYGENASE ; PREVENTION
英文摘要

Honokiol is a bioactive compound extracted from the Chinese medicinal herb Magnolia officinalis. We recently demonstrated that honokiol inhibited arterial thrombosis through stimulation of prostacyclin (PGI(2)) generation and endothelial cell protection. The current study is designed to investigate its mechanism of stimulation of PGI(2) generation and cell protection. 6-keto-PGF1 alpha, the stable metabolite of PGI(2), in the media of rat aortic endothelial cells was measured with radio immunoassay kits. Indomethacin, an inhibitor of cyclooxygenase (COX) and tranylcypromine, a prostacyclin synthease inhibitor were used to ascertain the target enzyme affected by honokiol. Prostacyclin synthease protein levels in endothelial cells were determined by Western blot analysis using an anti-PGI(2) synthease rabbit polyclonal antibody. Flow cytometry was used to quantify the apoptotic cells and spectrophotometry was used to test the caspase-3 activity. Honokiol (0.376-37.6 mu M) increased the level of 6-keto-PGF1 alpha in the media of normal endothelial cells. It counteracted the inhibitory effect of tranylcypromine on the PGI(2) generation, but did not influence the effect of indomethacin; evidently, honokiol up-regulated the expression of prostacyclin synthease in the endothelial cells. These effects showed perfect concentration-dependent behavior. In addition, at lower concentration (0.376-3.76 mu M), honokiol significantly decreased the percentage of apoptotic endothelial cells induced by oxidized low-density lipoprotem (ox-LDL) and significantly lowered the activity of caspase-3 stimulated by ox-LDL. A high dose of honokiol (37.6 mu M), however, failed to influence either of them. In conclusion, honokiol augments PGI(2) generation in normal endothelial cells; its effect on PGI(2) generation attributes to up-regulation of prostacyclin synthease expression; its cell protection may be correlated with its inhibition on apoptosis of endothelial cells. These findings have partly revealed the molecular mechanism of honokiol on inhibiting arterial thrombosis. (c) 2006 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000243980300001
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63914
专题北京大学药学院_分子与细胞药理学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmacol, Beijing 100083, Peoples R China
2.Peking Univ, Dept Nat Med Chem, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, Xiaoxue,Chen, Shizhong,Wang, Yinye. Honokiol up-regulates prostacyclin synthease protein expression an inhibits endothelial cell apoptosis[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2007,554(1):1-7.
APA Zhang, Xiaoxue,Chen, Shizhong,&Wang, Yinye.(2007).Honokiol up-regulates prostacyclin synthease protein expression an inhibits endothelial cell apoptosis.EUROPEAN JOURNAL OF PHARMACOLOGY,554(1),1-7.
MLA Zhang, Xiaoxue,et al."Honokiol up-regulates prostacyclin synthease protein expression an inhibits endothelial cell apoptosis".EUROPEAN JOURNAL OF PHARMACOLOGY 554.1(2007):1-7.
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