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学科主题: 药学
题名:
Site-Specific RNase A Activity Was Dramatically Reduced in Serum from Multiple Types of Cancer Patients
作者: Huang, Weiyan1; Zhao, Mei2,3,4; Wei, Na1; Wang, Xiaoxia1; Cao, Huqing1; Du, Quan5,6; Liang, Zicai1
刊名: PLOS ONE
发表日期: 2014-05-07
DOI: 10.1371/journal.pone.0096490
卷: 9, 期:5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: RIBONUCLEASE-ACTIVITY ; PANCREATIC-CANCER ; A SUPERFAMILY ; HOST-DEFENSE ; PURIFICATION ; DIAGNOSIS ; SIRNA ; STABILITY ; SEQUENCE ; TUMOURS
英文摘要:

Potent RNase activities were found in the serum of mammals but the physiological function of the RNases was never well illustrated, largely due to the caveats in methods of RNase activity measurement. None of the existing methods can distinguish between RNases with different target specificities. A systematic study was recently carried out in our lab to investigate the site-specificity of serum RNases on double-stranded RNA substrates, and found that serum RNases cleave double- stranded RNAs predominantly at 5 ′-U/A-3 ′ and 5 ′-C/A-3 ′ dinucleotide sites, in a manner closely resembling RNase A. Based on this finding, a FRET assay was developed in the current study to measure this site-specific serum RNase activity in human samples using a double stranded RNA substrate. We demonstrated that the method has a dynamic range of 10(-5) mg/ml- 10(-1) mg/ml using serial dilution of RNase A. The sera of 303 cancer patients were subjected to comparison with 128 healthy controls, and it was found that serum RNase activities visualized with this site-specific double stranded probe were found to be significantly reduced in patients with gastric cancer, liver cancer, pancreatic cancer, esophageal cancer, ovary cancer, cervical cancer, bladder cancer, kidney cancer and lung cancer, while only minor changes were found in breast and colon cancer patients. This is the first report using double stranded RNA as probe to quantify site-specific activities of RNase A in a serum. The results illustrated that RNase A might be further evaluated to determine if it can serve as a new class of biomarkers for certain cancer types.

语种: 英语
所属项目编号: 5132015 ; 2011CBA01102 ; 2012AA022501 ; 2011B090400478 ; 20090001110052 ; 200800010019
项目资助者: Beijing Natural Science Foundation ; National Basic Research Program of China ; National High-tech R&amp ; D Program of China ; Guangdong Science and Technology Department ; Department of Education of China
WOS记录号: WOS:000335728900062
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63915
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China
2.Chinese Acad Med Sci, Canc Inst & Hosp, Dept Etiol & Carcinogenesis, Beijing 100730, Peoples R China
3.Chinese Acad Med Sci, Canc Inst & Hosp, State Key Lab Mol Oncol, Beijing 100730, Peoples R China
4.Peking Union Med Coll, Beijing 100021, Peoples R China
5.Peking Univ, Sch Pharmaceut Sci, Inst Mol Med, Beijing 100871, Peoples R China
6.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

Recommended Citation:
Huang, Weiyan,Zhao, Mei,Wei, Na,et al. Site-Specific RNase A Activity Was Dramatically Reduced in Serum from Multiple Types of Cancer Patients[J]. PLOS ONE,2014,9(5).
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