IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique
Chen, Zhanguang1; Qian, Sihua1; Chen, Xi2; Chen, Junhui3; Zhang, Guomin1; Zeng, Guihua1
关键词Resonance light scattering Paclitaxel Doxorubicin Epirubicin Mitoxantrone
刊名MICROCHIMICA ACTA
2012-04-01
DOI10.1007/s00604-011-0747-4
177期:1-2页:67-73
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]FLOW-INJECTION ANALYSIS ; NUCLEIC-ACIDS ; RAYLEIGH-SCATTERING ; SENSITIVE ASSAY ; BREAST-CANCER ; PROTEINS ; CARDIOTOXICITY ; DOXORUBICIN ; NANOGRAMS
英文摘要

We have developed a method to investigate the interaction between DNA-targeted anthracyclines and DNA in the presence of the drug paclitaxel. It is based on resonance light scattering (RLS) and on the finding that anthracyclines when bound to DNA undergo a dramatic enhancement in their RLS intensities, while paclitaxel does not display such an effect. However, the RLS intensities of the anthracyclines-DNA associates are remarkably enhanced again on addition of paclitaxel. UV-visible spectra reveal interactions between paclitaxel and anthracyclines, but no reaction between paclitaxel and DNA. Consequently, paclitaxel, though not DNA-targeted, can improve the DNA-binding capabilities of anthracyclines. Binding constants between anthracyclines and DNA, and improved efficiency of paclitaxel on the DNA-binding capabilities of anthracyclines were calculated. The DNA binding constants of doxorubicin, epirubicin, and mitoxantrone, respectively, are 4.53 x 10(5) L mol(-1), 6.05 x 10(5) L mol(-1), and 9.47 x 10(5) L mol(-1). The improved values in presence of paclitaxel are 78%, 47% and 19%. We also have investigated the effects of drug concentrations and the order of adding the drugs. Displacement studies (using methylene blue as a competitive agent) provided additional information on the mechanisms of the interaction between paclitaxel and anthracyclines.

语种英语
WOS记录号WOS:000304134700008
项目编号2006B35630009 ; YR09009
资助机构Guangdong Science and Technology Department ; Science Foundation of Shantou University
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63922
专题北京大学深圳医院
作者单位1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China
3.Peking Univ, Dept Radiol, Shenzhen Hosp, Shenzhen 518036, Peoples R China
推荐引用方式
GB/T 7714
Chen, Zhanguang,Qian, Sihua,Chen, Xi,et al. Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique[J]. MICROCHIMICA ACTA,2012,177(1-2):67-73.
APA Chen, Zhanguang,Qian, Sihua,Chen, Xi,Chen, Junhui,Zhang, Guomin,&Zeng, Guihua.(2012).Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique.MICROCHIMICA ACTA,177(1-2),67-73.
MLA Chen, Zhanguang,et al."Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique".MICROCHIMICA ACTA 177.1-2(2012):67-73.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, Zhanguang]的文章
[Qian, Sihua]的文章
[Chen, Xi]的文章
百度学术
百度学术中相似的文章
[Chen, Zhanguang]的文章
[Qian, Sihua]的文章
[Chen, Xi]的文章
必应学术
必应学术中相似的文章
[Chen, Zhanguang]的文章
[Qian, Sihua]的文章
[Chen, Xi]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。