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学科主题: 临床医学
题名:
Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique
作者: Chen, Zhanguang1; Qian, Sihua1; Chen, Xi2; Chen, Junhui3; Zhang, Guomin1; Zeng, Guihua1
关键词: Resonance light scattering ; Paclitaxel ; Doxorubicin ; Epirubicin ; Mitoxantrone
刊名: MICROCHIMICA ACTA
发表日期: 2012-04-01
DOI: 10.1007/s00604-011-0747-4
卷: 177, 期:1-2, 页:67-73
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: FLOW-INJECTION ANALYSIS ; NUCLEIC-ACIDS ; RAYLEIGH-SCATTERING ; SENSITIVE ASSAY ; BREAST-CANCER ; PROTEINS ; CARDIOTOXICITY ; DOXORUBICIN ; NANOGRAMS
英文摘要:

We have developed a method to investigate the interaction between DNA-targeted anthracyclines and DNA in the presence of the drug paclitaxel. It is based on resonance light scattering (RLS) and on the finding that anthracyclines when bound to DNA undergo a dramatic enhancement in their RLS intensities, while paclitaxel does not display such an effect. However, the RLS intensities of the anthracyclines-DNA associates are remarkably enhanced again on addition of paclitaxel. UV-visible spectra reveal interactions between paclitaxel and anthracyclines, but no reaction between paclitaxel and DNA. Consequently, paclitaxel, though not DNA-targeted, can improve the DNA-binding capabilities of anthracyclines. Binding constants between anthracyclines and DNA, and improved efficiency of paclitaxel on the DNA-binding capabilities of anthracyclines were calculated. The DNA binding constants of doxorubicin, epirubicin, and mitoxantrone, respectively, are 4.53 x 10(5) L mol(-1), 6.05 x 10(5) L mol(-1), and 9.47 x 10(5) L mol(-1). The improved values in presence of paclitaxel are 78%, 47% and 19%. We also have investigated the effects of drug concentrations and the order of adding the drugs. Displacement studies (using methylene blue as a competitive agent) provided additional information on the mechanisms of the interaction between paclitaxel and anthracyclines.

语种: 英语
所属项目编号: 2006B35630009 ; YR09009
项目资助者: Guangdong Science and Technology Department ; Science Foundation of Shantou University
WOS记录号: WOS:000304134700008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63922
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China
3.Peking Univ, Dept Radiol, Shenzhen Hosp, Shenzhen 518036, Peoples R China

Recommended Citation:
Chen, Zhanguang,Qian, Sihua,Chen, Xi,et al. Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique[J]. MICROCHIMICA ACTA,2012,177(1-2):67-73.
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