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IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Conversion of cadherin isoforms in cultured human gastric carcinoma cells
作者: Wang, Bing-Ling; Zhang, Zhi-Qian; Ke, Yang
关键词: E-cadherin ; N-cadherin ; transcription factor ; gastric cancer
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2006-02-14
卷: 12, 期:6, 页:966-970
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: EPITHELIAL-MESENCHYMAL TRANSITIONS ; REPRESSES E-CADHERIN ; SNAIL EXPRESSION ; BREAST-CANCER ; TUMOR-METASTASIS ; ADHESION SYSTEM ; N-CADHERIN ; IN-VITRO ; INVASION ; MORPHOGENESIS
英文摘要:

AIM: To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation.

METHODS: The expressions of cell adhesion molecules (including E-cadherin, N-cadherin, a-catenin, P-catenin) and cadherin transcription factors including snail, slug and twist were determined by reverse transcriptase-polymerase chain reaction(RT-PCR), immunoblotting and immunofluorescence in SV40-immortalized human gastric cell line Ges-1 and human gastric cancer cell lines MGC-803, BGC-823 and SGC-7901.

RESULTS: All cell lines expressed N-cadherin, but not E-cadherin. N-cadherin immunofluorescence was detected at cell membranous adherents junctions where co-localization with immunofluorescent staining of inner surface adhesion proteins alpha- and beta-catenins was observed. The transformed Ges-1 and gastric cancer cell lines all expressed transcription factors (snail, slug and twist) which inhibited the expression of E-cadherin and triggered epithelial-mesenchymal transformation.

CONCLUSION: Cadherin isoforms can change from E-cadherin to N-cadherin in transformed human gastric cancer cells, which is associated with intracellular events of stomach carcinogenesis and high expression of corresponding transcription factors. (c) 2006 The WJG Press. All rights reserved.

语种: 英语
WOS记录号: WOS:000239994700021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63924
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Beijing 100034, Peoples R China
2.Peking Univ, Beijing Inst Canc Res, Beijing 100034, Peoples R China

Recommended Citation:
Wang, Bing-Ling,Zhang, Zhi-Qian,Ke, Yang. Conversion of cadherin isoforms in cultured human gastric carcinoma cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2006,12(6):966-970.
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