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Suppression of early experimental osteoarthritis by in vivo delivery of the adenoviral vector-medated NF-kappa Bp65-specific siRNA
Chen, L. X.; Lin, L.; Wang, H. J.; Wei, X. L.; Fu, X.; Zhang, J. Y.; Yu, C. L.
关键词siRNA NF-kappa Bp65 adenoviral vector IL-1 beta TNF-alpha osteoarthritis
刊名OSTEOARTHRITIS AND CARTILAGE
2008-02-01
DOI10.1016/j.joca.2007.06.006
16期:2页:174-184
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics ; Rheumatology
研究领域[WOS]Orthopedics ; Rheumatology
关键词[WOS]INTERLEUKIN-1 RECEPTOR ANTAGONIST ; MEDIATED GENE-TRANSFER ; SMALL INTERFERING RNA ; NF-KAPPA-B ; MAMMALIAN-CELLS ; RHEUMATOID-ARTHRITIS ; GUINEA-PIGS ; EXPRESSION ; JOINT ; GROWTH
英文摘要

Objective: This study was to use adenoviral vector-mediated nuclear factor-kappa Bp65 (NF-kappa Bp65)-specific siRNA (Ad-siRNA(NF-kappa BP65)) to suppress the progression of early osteoarthritis (OA) in rat model, and therefore to explore a new gene therapy for OA.

Methods: Reverse transcription polymerase chain reaction was performed to confirm the silencing effect of Ad-siRNA(NF-kappa Bp65) in cultured rat chondrocytes. Transection of the medial collateral ligament plus partial medial meniscectomy was operated in the knee of rats to establish OA model. Histological analysis was made to assess the morphological change of cartilage and synovium, and enzyme-linked immunosorbent assay was made to measure the expression of cytokines, such as interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha), in synovial fluid. The silencing effect of Ad-siRNA(NF-kappa Bp65) on NF-kappa Bp65 in cartilage and synovium of knee was measured with Western blot and the activation of NF-kappa B was measured with electrophoretic mobility shift assays.

Results: Ad-siRNA(NF-kappa Bp65) can inhibit the activation of NF-kappa B and the expression of NF-kappa Bp65 in cartilage and synovium of the knee, restrain the induction of IL-1 beta and TNF-alpha in synovial fluid, alleviate the inflammation of synovium and reduce the degradation of cartilage in early phase of experimental OA.

Conclusions: Ad-siRNA(NF-kappa Bp61) can suppress the progression of the early experimental OA which suggests that Ad-siRNA(NF-kappa Bp65) has potential to be a useful preventive and therapeutic agent for CA. (c) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000253638500003
引用统计
被引频次:76[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63932
专题北京大学第三临床医学院_运动医学研究所
北京大学精神卫生研究所_护理部
作者单位Peking Univ, Hosp 3, Inst Sports Med, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Chen, L. X.,Lin, L.,Wang, H. J.,et al. Suppression of early experimental osteoarthritis by in vivo delivery of the adenoviral vector-medated NF-kappa Bp65-specific siRNA[J]. OSTEOARTHRITIS AND CARTILAGE,2008,16(2):174-184.
APA Chen, L. X..,Lin, L..,Wang, H. J..,Wei, X. L..,Fu, X..,...&Yu, C. L..(2008).Suppression of early experimental osteoarthritis by in vivo delivery of the adenoviral vector-medated NF-kappa Bp65-specific siRNA.OSTEOARTHRITIS AND CARTILAGE,16(2),174-184.
MLA Chen, L. X.,et al."Suppression of early experimental osteoarthritis by in vivo delivery of the adenoviral vector-medated NF-kappa Bp65-specific siRNA".OSTEOARTHRITIS AND CARTILAGE 16.2(2008):174-184.
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