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学科主题: 临床医学
题名:
The expression and role of hybrid insulin/insulin-like growth factor receptor type 1 in endometrial carcinoma cells
作者: Zhang, Guo; Li, Xiaoping; Zhang, Lili; Zhao, Lijun; Jiang, Jing; Wang, Jianliu; Wei, Lihui
刊名: CANCER GENETICS AND CYTOGENETICS
发表日期: 2010-07-15
DOI: 10.1016/j.cancergencyto.2010.04.007
卷: 200, 期:2, 页:140-148
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Genetics & Heredity
研究领域[WOS]: Oncology ; Genetics & Heredity
关键词[WOS]: FACTOR-I RECEPTOR ; INSULIN-LIKE ; IGF-I ; ENDOTHELIAL-CELLS ; INCREASED ABUNDANCE ; CANCER ; DETERMINANTS ; PATHWAY ; BINDING
英文摘要:

Insulin receptor (IR) and type 1 insulin-like growth factor receptor (IGF-IR) can assemble heteromerically as a hybrid insulin/IGF-I receptor (hybrid-R) in tissues that express both molecules. There is little information about hybrid-R in endometrial carcinoma, in which both IR and IGF-IR are frequently overexpressed. We used immunoprecipitation to detect hybrid-R expression in two endometrial carcinoma cell lines: HEC-1a, which has low estrogen receptor (ER) expression, and Ishikawa, which is positive for ER expression. To explore the role of hybrid-R in endometrial carcinoma cells, we examined phosphorylation of extracellular signal-regulated kinase (ERK1/2), which is a key molecule in the mitogen-activated protein kinase (MAPK) pathway. The effect of inhibiting IGF-I, IGF-II, and insulin on cell cycle progression and apoptosis was assessed by flow cytometry. Both cell lines expressed hybrid-R, and HEC-1a cells had higher expression levels than did Ishikawa cells. IGF-I induced ERK1/2 phosphorylation in HEC-1a cells mainly through hybrid-R; in Ishikawa cells, this effect was mediated only in part by hybrid-R. Insulin stimulated ERK1/2 phosphorylation partly through hybrid-R in HEC-1a cells, but not in Ishikawa cells. Both IGF′s and insulin increased cellular DNA content in the S phase of the cell cycle in HEC-1a through hybrid-R. In contrast, in Ishikawa cells, only insulin enhanced DNA content in S phase through hybrid-R. Both IGFs and insulin significantly decreased apoptosis in HEC-1a cells through hybrid-R, and a similar but moderate effect was observed in Ishikawa cells. Hybrid-R, which is present in endometrial carcinoma cells, may have an important role in mediating IGF- and insulin-induced cell growth and in preventing apoptosis. (C) 2010 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 200800010095 ; 30973181
项目资助者: Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China
WOS记录号: WOS:000279373500009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63941
Appears in Collections:北京大学第二临床医学院_妇科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Gynecol, Beijing 100044, Peoples R China

Recommended Citation:
Zhang, Guo,Li, Xiaoping,Zhang, Lili,et al. The expression and role of hybrid insulin/insulin-like growth factor receptor type 1 in endometrial carcinoma cells[J]. CANCER GENETICS AND CYTOGENETICS,2010,200(2):140-148.
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