IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Continual Exposure to Cigarette Smoke Extracts Induces Tumor-Like Transformation of Human Nontumor Bronchial Epithelial Cells in a Microfluidic Chip
Li, Encheng1; Xu, Zhiyun1; Liu, Fen1; Wang, Huiling1; Wen, Jiabin1; Shao, Shujuan2; Zhang, Lichuan3; Wang, Lei4; Liu, Chong4; Lu, Jianxin5; Wang, Wenxin6; Gao, Zhancheng7; Wang, Qi1
关键词Smoking Transformation Epithelial cells Reactive oxygen species Microfluidic chip
刊名JOURNAL OF THORACIC ONCOLOGY
2014-08-01
9期:8页:1091-1100
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Respiratory System
研究领域[WOS]Oncology ; Respiratory System
关键词[WOS]OBSTRUCTIVE PULMONARY-DISEASE ; LUNG-CANCER ; OXIDATIVE STRESS ; DOWN-REGULATION ; FOLLOW-UP ; INFLAMMATION ; ACTIVATION ; PROLIFERATION ; TUMORIGENESIS ; SENSITIVITY
英文摘要

Introduction: Heavy cigarette smoking-related chronic obstructive pulmonary disease is an independent risk factor for lung squamous carcinoma. However, the mechanisms underlying the malignant transformation of bronchial epithelial cells are unclear.

Methods: In our study, human tumor-adjacent bronchial epithelial cells were obtained from 10 cases with smoking-related chronic obstructive pulmonary disease and lung squamous carcinoma and cultured in an established microfluidic chip for continual exposure to cigarette smoke extracts (CSE) to investigate the potential tumor-like transformation and mechanisms. The integrated microfluidic chip included upstream concentration gradient generator and downstream cell culture chambers supplied by flowing medium containing different concentrations of CSE.

Results: Our results showed that continual exposure to low doses of CSE promoted cell proliferation whereas to high doses of CSE triggered cell apoptosis. Continual exposure to CSE promoted reactive oxygen species production in human epithelial cells in a dose-dependent manner. More importantly, continual exposure to low dose of CSE promoted the epithelial-to-mesenchymal transition process and anchorage-independent growth, and increased chromosome instability in bronchial epithelial cells, accompanied by activating the GRP78, NF-kappa B, and PI3K pathways.

Conclusions: The established microfluidic chip is suitable for primary culture of human tumor-adjacent bronchial epithelial cells to investigate the malignant transformation. Continual exposure to low doses of CSE promoted tumor-like transformation of human nontumor bronchial epithelial cells by inducing reactive oxygen species production and activating the relevant signaling.

语种英语
WOS记录号WOS:000340138700010
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63971
Collection北京大学第二临床医学院
北京大学第二临床医学院_呼吸科
作者单位1.Dalian Med Univ, Affiliated Hosp 2, Dept Resp Med, Dalian, Peoples R China
2.Dalian Med Univ, Dept Histol & Embryol, Dalian, Peoples R China
3.Dalian Univ, Affiliated Zhongshan Hosp, Dept Resp Med, Dalian 116012, Peoples R China
4.Dalian Univ Technol, Key Lab Micro Nano Technol & Syst Liaoning Prov, Dalian, Peoples R China
5.Wenzhou Med Coll, Key Lab Lab Med, Zhejiang Prov Key Lab Med Genet, Minist Educ, Wenzhou, Zhejiang, Peoples R China
6.Univ Coll Dublin, Sch Med & Med Sci, Charles Inst Dermatol, Dublin, Ireland
7.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Li, Encheng,Xu, Zhiyun,Liu, Fen,et al. Continual Exposure to Cigarette Smoke Extracts Induces Tumor-Like Transformation of Human Nontumor Bronchial Epithelial Cells in a Microfluidic Chip[J]. JOURNAL OF THORACIC ONCOLOGY,2014,9(8):1091-1100.
APA Li, Encheng.,Xu, Zhiyun.,Liu, Fen.,Wang, Huiling.,Wen, Jiabin.,...&Wang, Qi.(2014).Continual Exposure to Cigarette Smoke Extracts Induces Tumor-Like Transformation of Human Nontumor Bronchial Epithelial Cells in a Microfluidic Chip.JOURNAL OF THORACIC ONCOLOGY,9(8),1091-1100.
MLA Li, Encheng,et al."Continual Exposure to Cigarette Smoke Extracts Induces Tumor-Like Transformation of Human Nontumor Bronchial Epithelial Cells in a Microfluidic Chip".JOURNAL OF THORACIC ONCOLOGY 9.8(2014):1091-1100.
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