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IR@PKUHSC  > 北京大学药学院  > 天然药物学系  > 期刊论文
学科主题: 药学
题名:
Identification of Cistanche Species by Chemical and Inter-simple Sequence Repeat Fingerprinting
作者: Shi, Hai Ming2; Wang, Shi Jing2; Wang, Meng Yue2; Tu, Peng Fei1; Li, Xiao Bo2
关键词: authentic identification ; Cistanche ; fingerprint ; HPLC ; inter-simple sequence repeat
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2009
卷: 32, 期:1, 页:142-146
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: PHENYLETHANOID GLYCOSIDES ; SPACER ; HERBA ; SALSA ; CHINA ; MICE
英文摘要:

Herba Cistanches is a common traditional Chinese medicine that has been used to reinforce the vital function of kidney and induce laxation for more than two thousands years. Four Cistanche species were found as Herba Cistanches in China herbal markets, including C. deserticola, C. tubulosa, C. salsa and C sinensis. Phenylethanoid glycosides, particularly echinacoside and acteoside, are considered as active ingredients in Cistanche species. The contents of these compounds showed variation in different species and geographical sources. Standard chemical fingerprints were generated from each of four Cistanche species, which could be identification markers. In genetic analysis of Cistanche species, ninety-four inter-simple sequence repeat (ISSR) primers were used for polymerase chain reaction (PCR) amplification, and of which eight primers were found to be sufficient to distinguish different Cistanche species. As a result, the chemical fingerprint combined with the genetic fingerprint for distinction of Cistanche species could serve as markers for quality control of Herba Cistanches.

语种: 英语
所属项目编号: 30371042
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000262069200026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63972
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China

Recommended Citation:
Shi, Hai Ming,Wang, Shi Jing,Wang, Meng Yue,et al. Identification of Cistanche Species by Chemical and Inter-simple Sequence Repeat Fingerprinting[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2009,32(1):142-146.
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