学科主题基础医学
Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines
Peng, HM; Chen, GA
关键词embryonic stem cell derivation passage serum-free cultivation
刊名HUMAN REPRODUCTION
2006
DOI10.1093/humrep/dei275
21期:1页:217-222
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]PLURIPOTENTIAL CELLS ; HUMAN BLASTOCYSTS ; MOUSE EMBRYOS ; GERM-LINE ; IN-VITRO ; DIFFERENTIATION ; CARDIOMYOCYTES ; DERIVATION ; CULTURE
英文摘要

BACKGROUND: Serum-containing and serum-free media were used to derive human embryonic stem (HES) cells from donated oocytes and embryos. METHODS and RESULTS: Inner cell masses (ICM) were isolated by immunosurgery. The HES cells were found to be easily obtained and expanded in a serum-free medium. The efficacy in establishing human embryonic stem cell lines improved in a serum-free medium compared with that in serum-containing media. Four HES cell lines were derived from 13 isolated ICM on mouse embryonic fibroblast feeder layers. All four cell lines possess the same characteristics and differentiating potency: normal 46, XX or 46, XY karyotype; and expressing a series of surface markers such as APase, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, but not SSEA-1. They can form embryoid bodies in suspension culture and develop teratomas comprising derivatives of three embryonic germ layers when injected into severe combined immunodeficient mice. CONCLUSION: These preliminary results suggest that serum-free cultivation may be superior to serum-containing cultivation for deriving human embryonic stem cells.

语种英语
WOS记录号WOS:000233846700030
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被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63976
专题北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100083, Peoples R China
2.Gen Hosp PLA, Dept Obstet & Gynecol, Beijing 100853, Peoples R China
3.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China
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Peng, HM,Chen, GA. Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines[J]. HUMAN REPRODUCTION,2006,21(1):217-222.
APA Peng, HM,&Chen, GA.(2006).Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines.HUMAN REPRODUCTION,21(1),217-222.
MLA Peng, HM,et al."Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines".HUMAN REPRODUCTION 21.1(2006):217-222.
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