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学科主题: 基础医学
题名:
Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines
作者: Peng, HM; Chen, GA
关键词: embryonic stem cell ; derivation ; passage ; serum-free cultivation
刊名: HUMAN REPRODUCTION
发表日期: 2006
DOI: 10.1093/humrep/dei275
卷: 21, 期:1, 页:217-222
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: PLURIPOTENTIAL CELLS ; HUMAN BLASTOCYSTS ; MOUSE EMBRYOS ; GERM-LINE ; IN-VITRO ; DIFFERENTIATION ; CARDIOMYOCYTES ; DERIVATION ; CULTURE
英文摘要:

BACKGROUND: Serum-containing and serum-free media were used to derive human embryonic stem (HES) cells from donated oocytes and embryos. METHODS and RESULTS: Inner cell masses (ICM) were isolated by immunosurgery. The HES cells were found to be easily obtained and expanded in a serum-free medium. The efficacy in establishing human embryonic stem cell lines improved in a serum-free medium compared with that in serum-containing media. Four HES cell lines were derived from 13 isolated ICM on mouse embryonic fibroblast feeder layers. All four cell lines possess the same characteristics and differentiating potency: normal 46, XX or 46, XY karyotype; and expressing a series of surface markers such as APase, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, but not SSEA-1. They can form embryoid bodies in suspension culture and develop teratomas comprising derivatives of three embryonic germ layers when injected into severe combined immunodeficient mice. CONCLUSION: These preliminary results suggest that serum-free cultivation may be superior to serum-containing cultivation for deriving human embryonic stem cells.

语种: 英语
WOS记录号: WOS:000233846700030
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63976
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100083, Peoples R China
2.Gen Hosp PLA, Dept Obstet & Gynecol, Beijing 100853, Peoples R China
3.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Peng, HM,Chen, GA. Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines[J]. HUMAN REPRODUCTION,2006,21(1):217-222.
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