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Alteration of cholinergic and peptidergic neurotransmitters in rat ileum induced by acute stress following transient intestinal infection is mast cell dependent
Leng Yu-xin1; Wei Yan-yu1; Chen Hong1; Zhou Shu-pei2; Yang Yan-lin3; Duan Li-ping1
关键词mast cells transient infection acute cold restriction stress acetylcholine substance P
刊名CHINESE MEDICAL JOURNAL
2010-01-20
DOI10.3760/cma.j.issn.0366-6999.2010.02.020
123期:2页:227-233
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]IRRITABLE-BOWEL-SYNDROME ; PSYCHOLOGICAL STRESS ; ACETYLTRANSFERASE ACTIVITY ; MUSCLE HYPERCONTRACTILITY ; VISCERAL HYPERALGESIA ; TRICHINELLA-SPIRALIS ; ABDOMINAL-PAIN ; RECEPTOR ; PATHOGENESIS ; INFLAMMATION
英文摘要

Background Mast cells are implicated in the development of irritable bowel syndrome (IBS), which is associated with the activation of the "neural-immune" system. The aim of this study was to investigate the role of mast cells in the remodeling of cholinergic and peptidergic neurotransmitters induced by acute cold restriction stress (ACRS) post infection (PI) using mast cell deficient rats (Ws/Ws) and their wild-type controls (+/+).

Methods Transient intestinal infection was initiated by giving 1500 Trichinella spiralis (TS.) larvae by gavage. ACRS was induced for 2 hours at day 100 PI. Samples of terminal ilea were prepared for H&E staining, mast cell counting and activation and assessment of IL-1 beta and IL-10.

Results When infected, both strains of rats experienced an acute infectious stage followed by a recovery. Histological scores were significantly higher in infected rats compared with those of the non-infected controls at day 10 PI (10 day-PI vs. control: +/+: 2.75 +/- 0.17 vs. 0.42 +/- 0.09; Ws/Ws: 2.67 +/- 0.67 vs. 0.50 +/- 0.34; P <0.01). In +/+ rats, post-infection ACRS induced the formation of low-grade inflammation, represented by the imbalance of IL-1 beta and IL-10 (IL-1 beta: PI+ACRS vs. control: (1812.24 +/- 561.61) vs. (1275.97 +/- 410.21) pg/g, P <0.05; IL-10: PI+ACRS vs. control: (251.9 +/- 39.8) vs. (255.3 +/- 24.7) pg/g, P >0.05), accompanied by hyperplasia and activation of mast cells (PI+ACRS vs. control: 58.8 +/- 19.2 vs. 28.0 +/- 7.6; P <0.01). The balance between acetylcholine (ACh) and substance P (SP) was also disturbed (ACh: PI+ACRS vs. control: (743.94 +/- 238.72) vs. (1065.68 +/- 256.46) pg/g, P <0.05; SP: PI+ACRS vs. control: (892.60 +/- 231.12) vs. (696.61 +/- 148.61) pg/g, P <0.05). Nevertheless, similar changes of IL-1 beta/IL-10 and ACh/SP were not detected in Ws/Ws rats.

Conclusion The imbalance of ACh/SP, together with the activation of mucosal immunity induced by post-infection ACRS were lacking in mast cell deficient rats, which supports the premise that mast cells play an important role in cholinergic and peptidergic remodeling in the ileum of rats. Chin Med J 2010;123(2):227-233

语种英语
WOS记录号WOS:000274319800020
项目编号30470776 ; 7082102
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63980
专题北京大学第三临床医学院_消化科
北京大学医学部管理机构_科学研究处
实验动物科学部
北京大学第二临床医学院_心血管内科
北京大学第三临床医学院_危重医学科
作者单位1.Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Lab Anim Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Med Res Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Leng Yu-xin,Wei Yan-yu,Chen Hong,et al. Alteration of cholinergic and peptidergic neurotransmitters in rat ileum induced by acute stress following transient intestinal infection is mast cell dependent[J]. CHINESE MEDICAL JOURNAL,2010,123(2):227-233.
APA Leng Yu-xin,Wei Yan-yu,Chen Hong,Zhou Shu-pei,Yang Yan-lin,&Duan Li-ping.(2010).Alteration of cholinergic and peptidergic neurotransmitters in rat ileum induced by acute stress following transient intestinal infection is mast cell dependent.CHINESE MEDICAL JOURNAL,123(2),227-233.
MLA Leng Yu-xin,et al."Alteration of cholinergic and peptidergic neurotransmitters in rat ileum induced by acute stress following transient intestinal infection is mast cell dependent".CHINESE MEDICAL JOURNAL 123.2(2010):227-233.
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