|Alteration of cholinergic and peptidergic neurotransmitters in rat ileum induced by acute stress following transient intestinal infection is mast cell dependent|
|Leng Yu-xin1; Wei Yan-yu1; Chen Hong1; Zhou Shu-pei2; Yang Yan-lin3; Duan Li-ping1|
|关键词||mast cells transient infection acute cold restriction stress acetylcholine substance P|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||IRRITABLE-BOWEL-SYNDROME ; PSYCHOLOGICAL STRESS ; ACETYLTRANSFERASE ACTIVITY ; MUSCLE HYPERCONTRACTILITY ; VISCERAL HYPERALGESIA ; TRICHINELLA-SPIRALIS ; ABDOMINAL-PAIN ; RECEPTOR ; PATHOGENESIS ; INFLAMMATION|
Background Mast cells are implicated in the development of irritable bowel syndrome (IBS), which is associated with the activation of the "neural-immune" system. The aim of this study was to investigate the role of mast cells in the remodeling of cholinergic and peptidergic neurotransmitters induced by acute cold restriction stress (ACRS) post infection (PI) using mast cell deficient rats (Ws/Ws) and their wild-type controls (+/+).
Methods Transient intestinal infection was initiated by giving 1500 Trichinella spiralis (TS.) larvae by gavage. ACRS was induced for 2 hours at day 100 PI. Samples of terminal ilea were prepared for H&E staining, mast cell counting and activation and assessment of IL-1 beta and IL-10.
Results When infected, both strains of rats experienced an acute infectious stage followed by a recovery. Histological scores were significantly higher in infected rats compared with those of the non-infected controls at day 10 PI (10 day-PI vs. control: +/+: 2.75 +/- 0.17 vs. 0.42 +/- 0.09; Ws/Ws: 2.67 +/- 0.67 vs. 0.50 +/- 0.34; P <0.01). In +/+ rats, post-infection ACRS induced the formation of low-grade inflammation, represented by the imbalance of IL-1 beta and IL-10 (IL-1 beta: PI+ACRS vs. control: (1812.24 +/- 561.61) vs. (1275.97 +/- 410.21) pg/g, P <0.05; IL-10: PI+ACRS vs. control: (251.9 +/- 39.8) vs. (255.3 +/- 24.7) pg/g, P >0.05), accompanied by hyperplasia and activation of mast cells (PI+ACRS vs. control: 58.8 +/- 19.2 vs. 28.0 +/- 7.6; P <0.01). The balance between acetylcholine (ACh) and substance P (SP) was also disturbed (ACh: PI+ACRS vs. control: (743.94 +/- 238.72) vs. (1065.68 +/- 256.46) pg/g, P <0.05; SP: PI+ACRS vs. control: (892.60 +/- 231.12) vs. (696.61 +/- 148.61) pg/g, P <0.05). Nevertheless, similar changes of IL-1 beta/IL-10 and ACh/SP were not detected in Ws/Ws rats.
Conclusion The imbalance of ACh/SP, together with the activation of mucosal immunity induced by post-infection ACRS were lacking in mast cell deficient rats, which supports the premise that mast cells play an important role in cholinergic and peptidergic remodeling in the ileum of rats. Chin Med J 2010;123(2):227-233
|项目编号||30470776 ; 7082102|
|资助机构||National Natural Science Foundation of China ; Beijing Natural Science Foundation|
|作者单位||1.Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100191, Peoples R China|
2.Peking Univ, Hlth Sci Ctr, Dept Lab Anim Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Med Res Ctr, Beijing 100191, Peoples R China
|Leng Yu-xin,Wei Yan-yu,Chen Hong,et al. Alteration of cholinergic and peptidergic neurotransmitters in rat ileum induced by acute stress following transient intestinal infection is mast cell dependent[J]. CHINESE MEDICAL JOURNAL,2010,123(2):227-233.|
|APA||Leng Yu-xin,Wei Yan-yu,Chen Hong,Zhou Shu-pei,Yang Yan-lin,&Duan Li-ping.(2010).Alteration of cholinergic and peptidergic neurotransmitters in rat ileum induced by acute stress following transient intestinal infection is mast cell dependent.CHINESE MEDICAL JOURNAL,123(2),227-233.|
|MLA||Leng Yu-xin,et al."Alteration of cholinergic and peptidergic neurotransmitters in rat ileum induced by acute stress following transient intestinal infection is mast cell dependent".CHINESE MEDICAL JOURNAL 123.2(2010):227-233.|