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Ginsenoside Rg1 inhibits autophagy in H9c2 cardiomyocytes exposed to hypoxia/reoxygenation
Zhang, Zi-Long; Fan, Yan; Liu, Mei-Lin
关键词Ginsenoside Rg1 Autophagy AMPK alpha H9c2 cells Hypoxia/reoxygenation
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
2012-06-01
DOI10.1007/s11010-012-1265-3
365期:1-2页:243-250
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
资助者National Science and Technology Major Projects "Major New Drugs Innovation and Development&prime ; &prime ; National Science and Technology Major Projects "Major New Drugs Innovation and Development&prime ; &prime
研究领域[WOS]Cell Biology
关键词[WOS]CELL-DEATH ; MYOCARDIAL ISCHEMIA/REPERFUSION ; GLUCOSE DEPRIVATION ; RAT CARDIOMYOCYTE ; CARDIAC MYOCYTES ; PROTEIN-KINASE ; INJURY ; HEART ; MECHANISMS ; STRESS
英文摘要

Ginsenoside Rg1 promotes antioxidative protection and intracellular calcium homeostasis in cardiomyocytes hypoxia/reoxygenation (H/R) model. However, the pharmacological effects of G-Rg1 on autophagy in cardiomyocytes have not been reported. In this study, we employed H9c2 cardiomyocytes as a model to investigate the effects of G-Rg1 on autophagy in cardiomyocytes under H/R stress. Our results showed that H/R induced increased level of LC3B-2, an autophagy marker, in a time-dependent manner in association with decreased cell viability and cellular ATP content. H/R-induced autophagy and apoptosis were further confirmed by morphological examination. 100 mu mol/l Rg1-inhibited H/R induced autophagy and apoptosis, and this was associated with the increase of cellular ATP content and the relief of oxidative stress in the cells. Mechanistically, we found that Rg1 inhibited the activation of AMPK alpha, promoted the activation of mTOR, and decreased the levels of LC3B-2 and Beclin-1. In conclusion, our data suggest that H/R induces autophagy in H9c2 cells leading to cell injury. Rg1 inhibits autophagosomal formation and apoptosis in the cells, which may be beneficial to the survival of cardiomyocytes under H/R.

语种英语
所属项目编号2009ZX09103-441
资助者National Science and Technology Major Projects "Major New Drugs Innovation and Development&prime ; &prime ; National Science and Technology Major Projects "Major New Drugs Innovation and Development&prime ; &prime
WOS记录号WOS:000303472400027
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63988
专题北京大学第一临床医学院_老年病内科
作者单位Peking Univ, Dept Geriatr, Hosp 1, Beijing 100034, Peoples R China
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Zhang, Zi-Long,Fan, Yan,Liu, Mei-Lin. Ginsenoside Rg1 inhibits autophagy in H9c2 cardiomyocytes exposed to hypoxia/reoxygenation[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2012,365(1-2):243-250.
APA Zhang, Zi-Long,Fan, Yan,&Liu, Mei-Lin.(2012).Ginsenoside Rg1 inhibits autophagy in H9c2 cardiomyocytes exposed to hypoxia/reoxygenation.MOLECULAR AND CELLULAR BIOCHEMISTRY,365(1-2),243-250.
MLA Zhang, Zi-Long,et al."Ginsenoside Rg1 inhibits autophagy in H9c2 cardiomyocytes exposed to hypoxia/reoxygenation".MOLECULAR AND CELLULAR BIOCHEMISTRY 365.1-2(2012):243-250.
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