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PCR microchip array based on polymer bonding technique
Yu, XM; Li, T; Hao, L; Zhang, DC
关键词PCR microchip array polymer bonding DNA amplification
刊名JOURNAL OF ELECTRONIC PACKAGING
2005-03-01
DOI10.1115/1.1849231
127期:1页:38-42
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Electrical & Electronic ; Engineering, Mechanical
研究领域[WOS]Engineering
关键词[WOS]AMPLIFICATION
英文摘要

A polymerase chain reaction (PCR) microchip array with a dimension of 21.3 x 17.5 mm(2) has been fabricated by the silicon micromachining technique. The chip is composed of 192 rectangular reaction chambers with the volume of 50 nL. In order to package the silicon-based PCR chip, a low-temperature bonding technique using polymers as the intermediate layers has been developed. The tested polymers include positive photoresist, polyimide, and epoxy. Using stamping and sandwich techniques, void-free and liquid-proof bondings of silicon to a glass cover were achieved with the three polymers. The experimental results of fluorescence-based PCR demonstrate that the DNA amplification can be performed in the chip arrays with nanoliter volumes and the polymer bonding technique is biocompatible and suitable,for the microchip packaging.

语种英语
WOS记录号WOS:000228418200008
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64003
专题北京大学第一临床医学院_血液内科
作者单位1.Peking Univ, Inst Microelect, Beijing 100871, Peoples R China
2.Peking Univ, First Hosp, Dept Hematol, Beijing 100034, Peoples R China
3.Peking Univ, Inst Microelect, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Yu, XM,Li, T,Hao, L,et al. PCR microchip array based on polymer bonding technique[J]. JOURNAL OF ELECTRONIC PACKAGING,2005,127(1):38-42.
APA Yu, XM,Li, T,Hao, L,&Zhang, DC.(2005).PCR microchip array based on polymer bonding technique.JOURNAL OF ELECTRONIC PACKAGING,127(1),38-42.
MLA Yu, XM,et al."PCR microchip array based on polymer bonding technique".JOURNAL OF ELECTRONIC PACKAGING 127.1(2005):38-42.
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