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Photouncaged Sequence-specific Interstrand DNA Cross-Linking with Photolabile 4-oxo-enal-modified Oligonucleotides
Sun, Jingjing; Tang, Xinjing
刊名SCIENTIFIC REPORTS
2015-05-28
DOI10.1038/srep10473
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]DUPLEX DNA ; SELECTIVE FORMATION ; IN-VITRO ; CYTIDINE ; ALKYLATION ; EFFICIENT ; REPAIR ; LIGHT ; SITE ; RNA
英文摘要

DNA cross-linking technology is an attractive tool for the detection, regulation, and manipulation of genes. In this study, a series of photolabile4-oxo-enal-modified oligonucleotides functionalized with photosensitive O-nitrobenzyl derivatives were rationally designed as a new kind of photocaged crosslinking agents. A comprehensive evaluation of cross-linking reactions for different nucleobases in complementary strands under different conditions suggested that the modified DNA oligonucleotides tended to form interstrand cross-linking to nucleobases with the potential of thymidine > guanosine >> cytidine similar to adenosine. Different from previous literature reports that cytidine and adenosine were preferential cross-linked nucleobases with 4-oxo-enal moieties, our study represents the first example of DNA cross-linking for T and G selectivity using 4-oxo-enal moiety. The cross-linked adducts were identified and their cross-linking mechanism was also illustrated. This greatly expands the applications of 4-oxo-enal derivatives in the studies of DNA damage and RNA structure

语种英语
WOS记录号WOS:000355546900001
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64004
专题北京大学药学院
北京大学医学部管理机构_医学部
北京大学深圳医院_神经内科
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Sun, Jingjing,Tang, Xinjing. Photouncaged Sequence-specific Interstrand DNA Cross-Linking with Photolabile 4-oxo-enal-modified Oligonucleotides[J]. SCIENTIFIC REPORTS,2015,5.
APA Sun, Jingjing,&Tang, Xinjing.(2015).Photouncaged Sequence-specific Interstrand DNA Cross-Linking with Photolabile 4-oxo-enal-modified Oligonucleotides.SCIENTIFIC REPORTS,5.
MLA Sun, Jingjing,et al."Photouncaged Sequence-specific Interstrand DNA Cross-Linking with Photolabile 4-oxo-enal-modified Oligonucleotides".SCIENTIFIC REPORTS 5(2015).
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