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学科主题: 药学
题名:
Photouncaged Sequence-specific Interstrand DNA Cross-Linking with Photolabile 4-oxo-enal-modified Oligonucleotides
作者: Sun, Jingjing; Tang, Xinjing
刊名: SCIENTIFIC REPORTS
发表日期: 2015-05-28
DOI: 10.1038/srep10473
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: DUPLEX DNA ; SELECTIVE FORMATION ; IN-VITRO ; CYTIDINE ; ALKYLATION ; EFFICIENT ; REPAIR ; LIGHT ; SITE ; RNA
英文摘要:

DNA cross-linking technology is an attractive tool for the detection, regulation, and manipulation of genes. In this study, a series of photolabile4-oxo-enal-modified oligonucleotides functionalized with photosensitive O-nitrobenzyl derivatives were rationally designed as a new kind of photocaged crosslinking agents. A comprehensive evaluation of cross-linking reactions for different nucleobases in complementary strands under different conditions suggested that the modified DNA oligonucleotides tended to form interstrand cross-linking to nucleobases with the potential of thymidine > guanosine >> cytidine similar to adenosine. Different from previous literature reports that cytidine and adenosine were preferential cross-linked nucleobases with 4-oxo-enal moieties, our study represents the first example of DNA cross-linking for T and G selectivity using 4-oxo-enal moiety. The cross-linked adducts were identified and their cross-linking mechanism was also illustrated. This greatly expands the applications of 4-oxo-enal derivatives in the studies of DNA damage and RNA structure

语种: 英语
所属项目编号: 2012CB720600 ; 2013CB933800 ; 21422201 ; 21372018 ; BMU 20110263
项目资助者: National Basic Research Program of China (&prime ; 973&prime ; Program) ; National Natural Science Foundation of China ; Innovation Team of Ministry of Education
WOS记录号: WOS:000355546900001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64004
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Sun, Jingjing,Tang, Xinjing. Photouncaged Sequence-specific Interstrand DNA Cross-Linking with Photolabile 4-oxo-enal-modified Oligonucleotides[J]. SCIENTIFIC REPORTS,2015,5.
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