IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajun in breast cancer cells
Fu, Yujie1,2; Kadioglu, Onat3; Wiench, Benjamin3; Wei, Zuofu1,2; Gao, Chang4; Luo, Meng1,2; Gu, Chengbo1,2; Zu, Yuangang1,2; Efferth, Thomas3
关键词Apoptosis Breast cancer Cell cycle Microarray Pharmacogenomics Fabaceae
刊名PHYTOMEDICINE
2015-04-15
DOI10.1016/j.phymed.2015.02.005
22期:4页:462-468
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]NATURAL COMPOUNDS ; GENE-EXPRESSION ; ENDOCRINE RESISTANCE ; ANTICANCER ACTIVITY ; SKIN-CANCER ; S PHASE ; STATISTICS ; GROWTH ; P21 ; BRCA2
英文摘要

Background: The low abundant cajanin stilbene acid (CSA) from Pigeon Pea (Cajanus cajan) has been shown to kill estrogen receptor alpha positive cancer cells in vitro and in vivo. Downstream effects such as cell cycle and apoptosis-related mechanisms have not been analyzed yet.

Material and methods: We analyzed the activity of CSA by means of flow cytometry (cell cycle distribution, mitochondrial membrane potential, MMP), confocal laser scanning microscopy (MMP), DNA fragmentation assay (apoptosis), Western blotting (Bax and Bcl-2 expression, caspase-3 activation) as well as mRNA microarray hybridization and Ingenuity pathway analysis.

Results: CSA induced G2/M arrest and apoptosis in a concentration dependent manner from 8.88 to 14.79 mu M. The MMP broke down, Bax was upregulated, Bcl-2 downregulated and caspase-3 activated. Microarray profiling revealed that CSA affected BRCA-related DNA damage response and cell cycle regulated chromosomal replication pathways.

Conclusion: CSA inhibited breast cancer cells by DNA damage and cell cycle related signaling pathways leading to cell cycle arrest and apoptosis. (C) 2015 Elsevier GmbH. All rights reserved.

语种英语
WOS记录号WOS:000353556300006
项目编号201004040 ; 2012-4-06 ; JC200704 ; MS2010DBLY031
资助机构Special Fund of Forestry Industrial Research for Public Welfare of China ; Importation of International Advanced Forestry Science and Technology, National Forestry Bureau ; Heilongjiang Province Science Foundation for Excellent Youths ; Project for Distinguished Teacher Abroad, Chinese Ministry of Education
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64013
专题北京大学第二临床医学院
作者单位1.Northeast Forestry Univ, Minist Educ, Key Lab Forest Plant Ecol, Harbin 150040, Peoples R China
2.Northeast Forestry Univ, Minist Educ, Engn Res Ctr Forest Biopreparat, Harbin 150040, Peoples R China
3.Johannes Gutenberg Univ Mainz, Inst Pharm & Biochem, Dept Pharmaceut Biol, D-55128 Mainz, Germany
4.Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Fu, Yujie,Kadioglu, Onat,Wiench, Benjamin,et al. Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajun in breast cancer cells[J]. PHYTOMEDICINE,2015,22(4):462-468.
APA Fu, Yujie.,Kadioglu, Onat.,Wiench, Benjamin.,Wei, Zuofu.,Gao, Chang.,...&Efferth, Thomas.(2015).Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajun in breast cancer cells.PHYTOMEDICINE,22(4),462-468.
MLA Fu, Yujie,et al."Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajun in breast cancer cells".PHYTOMEDICINE 22.4(2015):462-468.
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