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学科主题: 临床医学
题名:
Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajun in breast cancer cells
作者: Fu, Yujie1,2; Kadioglu, Onat3; Wiench, Benjamin3; Wei, Zuofu1,2; Gao, Chang4; Luo, Meng1,2; Gu, Chengbo1,2; Zu, Yuangang1,2; Efferth, Thomas3
关键词: Apoptosis ; Breast cancer ; Cell cycle ; Microarray ; Pharmacogenomics ; Fabaceae
刊名: PHYTOMEDICINE
发表日期: 2015-04-15
DOI: 10.1016/j.phymed.2015.02.005
卷: 22, 期:4, 页:462-468
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]: NATURAL COMPOUNDS ; GENE-EXPRESSION ; ENDOCRINE RESISTANCE ; ANTICANCER ACTIVITY ; SKIN-CANCER ; S PHASE ; STATISTICS ; GROWTH ; P21 ; BRCA2
英文摘要:

Background: The low abundant cajanin stilbene acid (CSA) from Pigeon Pea (Cajanus cajan) has been shown to kill estrogen receptor alpha positive cancer cells in vitro and in vivo. Downstream effects such as cell cycle and apoptosis-related mechanisms have not been analyzed yet.

Material and methods: We analyzed the activity of CSA by means of flow cytometry (cell cycle distribution, mitochondrial membrane potential, MMP), confocal laser scanning microscopy (MMP), DNA fragmentation assay (apoptosis), Western blotting (Bax and Bcl-2 expression, caspase-3 activation) as well as mRNA microarray hybridization and Ingenuity pathway analysis.

Results: CSA induced G2/M arrest and apoptosis in a concentration dependent manner from 8.88 to 14.79 mu M. The MMP broke down, Bax was upregulated, Bcl-2 downregulated and caspase-3 activated. Microarray profiling revealed that CSA affected BRCA-related DNA damage response and cell cycle regulated chromosomal replication pathways.

Conclusion: CSA inhibited breast cancer cells by DNA damage and cell cycle related signaling pathways leading to cell cycle arrest and apoptosis. (C) 2015 Elsevier GmbH. All rights reserved.

语种: 英语
所属项目编号: 201004040 ; 2012-4-06 ; JC200704 ; MS2010DBLY031
项目资助者: Special Fund of Forestry Industrial Research for Public Welfare of China ; Importation of International Advanced Forestry Science and Technology, National Forestry Bureau ; Heilongjiang Province Science Foundation for Excellent Youths ; Project for Distinguished Teacher Abroad, Chinese Ministry of Education
WOS记录号: WOS:000353556300006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64013
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Northeast Forestry Univ, Minist Educ, Key Lab Forest Plant Ecol, Harbin 150040, Peoples R China
2.Northeast Forestry Univ, Minist Educ, Engn Res Ctr Forest Biopreparat, Harbin 150040, Peoples R China
3.Johannes Gutenberg Univ Mainz, Inst Pharm & Biochem, Dept Pharmaceut Biol, D-55128 Mainz, Germany
4.Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Fu, Yujie,Kadioglu, Onat,Wiench, Benjamin,et al. Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajun in breast cancer cells[J]. PHYTOMEDICINE,2015,22(4):462-468.
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