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Protective effect of hydrogen sulfide on TNF-alpha and IFN-gamma-induced injury of intestinal epithelial barrier function in Caco-2 monolayers
Chen, Shan-wen; Zhu, Jing; Zuo, Shuai; Zhang, Jun-ling; Chen, Zi-yi; Chen, Guo-wei; Wang, Xin; Pan, Yi-sheng; Liu, Yu-cun; Wang, Peng-yuan
关键词H2S NF-kappa B Tight junction ZO-1 MLCK
刊名INFLAMMATION RESEARCH
2015-10-01
DOI10.1007/s00011-015-0862-5
64期:10页:789-797
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology
研究领域[WOS]Cell Biology ; Immunology
关键词[WOS]LIGHT-CHAIN KINASE ; TIGHT JUNCTION PERMEABILITY ; IN-VIVO ; PHOSPHORYLATION ; MECHANISM ; RATS ; DISRUPTION ; EXPRESSION ; INCREASE ; INFLAMMATION
英文摘要

Background and aim Studies have verified the protective effect of Hydrogen Sulfide (H2S) on gastric ulcer and ulcerative colitis, but the mechanisms are not fully illustrated. In this study, the possible protective effect of H2S on TNF-alpha/IFN-gamma induced barrier dysfunction was investigated in Caco-2 cell monolayers.

Method The barrier function of Caco-2 monolayers was evaluated by measuring trans-epithelial electrical resistance (TEER) and FITC-Dextran 4 kDa (FD-4) trans-membrane flux. ZO-1 and Occludin were chosen as markers of the localization of tight junction (TJ) proteins for immunofluorescence. The expression of MLCK and phosphorylation level of myosin light chain (MLC) were measured by immunoblotting. The activation of NF-kB p65 was analyzed by EMSA and immunofluorescence.

Results NaHS at 500uM significantly attenuated TNF-alpha/IFN-gamma-indueced Caco-2 monolayer barrier injury. The increased expression of MLCK and increased phosphorylation level of MLC induced by TNF-alpha/IFN-gamma was also inhibited significantly by NaHS. Additionally, NaHS inhibited TNF-alpha/IFN-gamma induced activation and nuclear translocation of NF-kB p65.

Conclusion The present study reveals the protective effect of H2S on TNF-alpha and IFN-gamma-induced injury of intestinal epithelial barrier function in Caco-2 monolayers and suggests that the suppression of MLCK-P-MLC signaling mediated by NF-kB P65 might be one of the mechanisms underlying the protective effect of H2S.

语种英语
WOS记录号WOS:000360997000006
资助机构China Health and Medical Development Foundation
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64016
专题北京大学第一临床医学院
北京大学第一临床医学院_普通外科
作者单位Peking Univ, Div Gen Surg, Peking Univ Hosp 1, Beijing 100034, Peoples R China
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Chen, Shan-wen,Zhu, Jing,Zuo, Shuai,et al. Protective effect of hydrogen sulfide on TNF-alpha and IFN-gamma-induced injury of intestinal epithelial barrier function in Caco-2 monolayers[J]. INFLAMMATION RESEARCH,2015,64(10):789-797.
APA Chen, Shan-wen.,Zhu, Jing.,Zuo, Shuai.,Zhang, Jun-ling.,Chen, Zi-yi.,...&Wang, Peng-yuan.(2015).Protective effect of hydrogen sulfide on TNF-alpha and IFN-gamma-induced injury of intestinal epithelial barrier function in Caco-2 monolayers.INFLAMMATION RESEARCH,64(10),789-797.
MLA Chen, Shan-wen,et al."Protective effect of hydrogen sulfide on TNF-alpha and IFN-gamma-induced injury of intestinal epithelial barrier function in Caco-2 monolayers".INFLAMMATION RESEARCH 64.10(2015):789-797.
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