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学科主题: 临床医学
题名:
Protective effect of hydrogen sulfide on TNF-alpha and IFN-gamma-induced injury of intestinal epithelial barrier function in Caco-2 monolayers
作者: Chen, Shan-wen; Zhu, Jing; Zuo, Shuai; Zhang, Jun-ling; Chen, Zi-yi; Chen, Guo-wei; Wang, Xin; Pan, Yi-sheng; Liu, Yu-cun; Wang, Peng-yuan
关键词: H2S ; NF-kappa B ; Tight junction ; ZO-1 ; MLCK
刊名: INFLAMMATION RESEARCH
发表日期: 2015-10-01
DOI: 10.1007/s00011-015-0862-5
卷: 64, 期:10, 页:789-797
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology
研究领域[WOS]: Cell Biology ; Immunology
关键词[WOS]: LIGHT-CHAIN KINASE ; TIGHT JUNCTION PERMEABILITY ; IN-VIVO ; PHOSPHORYLATION ; MECHANISM ; RATS ; DISRUPTION ; EXPRESSION ; INCREASE ; INFLAMMATION
英文摘要:

Background and aim Studies have verified the protective effect of Hydrogen Sulfide (H2S) on gastric ulcer and ulcerative colitis, but the mechanisms are not fully illustrated. In this study, the possible protective effect of H2S on TNF-alpha/IFN-gamma induced barrier dysfunction was investigated in Caco-2 cell monolayers.

Method The barrier function of Caco-2 monolayers was evaluated by measuring trans-epithelial electrical resistance (TEER) and FITC-Dextran 4 kDa (FD-4) trans-membrane flux. ZO-1 and Occludin were chosen as markers of the localization of tight junction (TJ) proteins for immunofluorescence. The expression of MLCK and phosphorylation level of myosin light chain (MLC) were measured by immunoblotting. The activation of NF-kB p65 was analyzed by EMSA and immunofluorescence.

Results NaHS at 500uM significantly attenuated TNF-alpha/IFN-gamma-indueced Caco-2 monolayer barrier injury. The increased expression of MLCK and increased phosphorylation level of MLC induced by TNF-alpha/IFN-gamma was also inhibited significantly by NaHS. Additionally, NaHS inhibited TNF-alpha/IFN-gamma induced activation and nuclear translocation of NF-kB p65.

Conclusion The present study reveals the protective effect of H2S on TNF-alpha and IFN-gamma-induced injury of intestinal epithelial barrier function in Caco-2 monolayers and suggests that the suppression of MLCK-P-MLC signaling mediated by NF-kB P65 might be one of the mechanisms underlying the protective effect of H2S.

语种: 英语
项目资助者: China Health and Medical Development Foundation
WOS记录号: WOS:000360997000006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64016
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: Peking Univ, Div Gen Surg, Peking Univ Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Chen, Shan-wen,Zhu, Jing,Zuo, Shuai,et al. Protective effect of hydrogen sulfide on TNF-alpha and IFN-gamma-induced injury of intestinal epithelial barrier function in Caco-2 monolayers[J]. INFLAMMATION RESEARCH,2015,64(10):789-797.
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