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学科主题: 临床医学
题名:
Ventana immunohistochemistry ALK (D5F3) detection of ALK expression in pleural effusion samples of lung adenocarcinoma
作者: Wang, Zheng1; Wu, Xiaonan2; Shi, Yuankai3; Han, Xiaohong3; Cheng, Gang2; Cui, Di1; Li, Lin2; Zhang, Yuhui4; Mu, Xinlin5; Zhang, Li6; Yang, Li1; Di, Jing1; Yu, Qi1; Liu, Dongge1
关键词: ALK ; diagnosis ; fluorescence in situ hybridization ; immunohistochemistry ; lung cancer ; NSCLC ; qRT-PCR
刊名: PERSONALIZED MEDICINE
发表日期: 2015
DOI: 10.2217/PME.15.3
卷: 12, 期:4, 页:349-357
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: GENE REARRANGEMENT ; CANCER PATIENTS ; EGFR MUTATIONS ; CRIZOTINIB ; FUSION ; FISH ; KRAS ; IDENTIFICATION ; INHIBITORS ; CARCINOMA
英文摘要:

Aim: To evaluate the Ventana IHC ALK (D5F3) assay for detecting anaplastic lymphoma kinase (ALK) protein expression in pleural effusion samples. Methods: Historical, selected (wild-type EGFR, K-RAS) pleural effusion cytologic blocks of lung adenocarcinoma samples (Study 1) and unselected lung adenocarcinoma pleural effusion cytologic blocks (Study 2) were tested by Ventana IHC ALK (D5F3) assay. Quantitative real-time-PCR was used to verify immunohistochemistry results. Results: A total of 17 out of 100 (Study 1) and ten out of 104 (Study 2) pleural effusion samples were ALK expression positive by the Ventana IHC ALK (D5F3) assay. The ALK fusion results with immunohistochemistry and quantitative real-time-PCR had a concordance rate of 87.5% ( = 0.886; p < 0.001). Conclusion: The Ventana IHC ALK (D5F3) assay is a reliable tool for detecting ALK protein expression in pleural effusion samples.

语种: 英语
所属项目编号: D141100000214003
项目资助者: Beijing Municipal Science and Technology commission
WOS记录号: WOS:000358945300005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64034
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Minist Hlth, Beijing Hosp, Dept Med Oncol, Beijing, Peoples R China
2.Minist Hlth, Beijing Hosp, Dept Pathol, Beijing, Peoples R China
3.Chinese Acad Med Sci, Canc Inst & Hosp, Dept Med Oncol, Beijing 100730, Peoples R China
4.Capital Med Univ, Beijing Inst Resp Med, Beijing Chao Yang Hosp, Beijing, Peoples R China
5.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100871, Peoples R China
6.Beijing Union Med Coll Hosp, Dept Resp Med, Beijing, Peoples R China

Recommended Citation:
Wang, Zheng,Wu, Xiaonan,Shi, Yuankai,et al. Ventana immunohistochemistry ALK (D5F3) detection of ALK expression in pleural effusion samples of lung adenocarcinoma[J]. PERSONALIZED MEDICINE,2015,12(4):349-357.
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