IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Ventana immunohistochemistry ALK (D5F3) detection of ALK expression in pleural effusion samples of lung adenocarcinoma
Wang, Zheng1; Wu, Xiaonan2; Shi, Yuankai3; Han, Xiaohong3; Cheng, Gang2; Cui, Di1; Li, Lin2; Zhang, Yuhui4; Mu, Xinlin5; Zhang, Li6; Yang, Li1; Di, Jing1; Yu, Qi1; Liu, Dongge1
关键词ALK diagnosis fluorescence in situ hybridization immunohistochemistry lung cancer NSCLC qRT-PCR
刊名PERSONALIZED MEDICINE
2015
DOI10.2217/PME.15.3
12期:4页:349-357
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]GENE REARRANGEMENT ; CANCER PATIENTS ; EGFR MUTATIONS ; CRIZOTINIB ; FUSION ; FISH ; KRAS ; IDENTIFICATION ; INHIBITORS ; CARCINOMA
英文摘要

Aim: To evaluate the Ventana IHC ALK (D5F3) assay for detecting anaplastic lymphoma kinase (ALK) protein expression in pleural effusion samples. Methods: Historical, selected (wild-type EGFR, K-RAS) pleural effusion cytologic blocks of lung adenocarcinoma samples (Study 1) and unselected lung adenocarcinoma pleural effusion cytologic blocks (Study 2) were tested by Ventana IHC ALK (D5F3) assay. Quantitative real-time-PCR was used to verify immunohistochemistry results. Results: A total of 17 out of 100 (Study 1) and ten out of 104 (Study 2) pleural effusion samples were ALK expression positive by the Ventana IHC ALK (D5F3) assay. The ALK fusion results with immunohistochemistry and quantitative real-time-PCR had a concordance rate of 87.5% ( = 0.886; p < 0.001). Conclusion: The Ventana IHC ALK (D5F3) assay is a reliable tool for detecting ALK protein expression in pleural effusion samples.

语种英语
WOS记录号WOS:000358945300005
项目编号D141100000214003
资助机构Beijing Municipal Science and Technology commission
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64034
专题北京大学第二临床医学院
北京大学第二临床医学院_呼吸科
作者单位1.Minist Hlth, Beijing Hosp, Dept Med Oncol, Beijing, Peoples R China
2.Minist Hlth, Beijing Hosp, Dept Pathol, Beijing, Peoples R China
3.Chinese Acad Med Sci, Canc Inst & Hosp, Dept Med Oncol, Beijing 100730, Peoples R China
4.Capital Med Univ, Beijing Inst Resp Med, Beijing Chao Yang Hosp, Beijing, Peoples R China
5.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100871, Peoples R China
6.Beijing Union Med Coll Hosp, Dept Resp Med, Beijing, Peoples R China
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GB/T 7714
Wang, Zheng,Wu, Xiaonan,Shi, Yuankai,et al. Ventana immunohistochemistry ALK (D5F3) detection of ALK expression in pleural effusion samples of lung adenocarcinoma[J]. PERSONALIZED MEDICINE,2015,12(4):349-357.
APA Wang, Zheng.,Wu, Xiaonan.,Shi, Yuankai.,Han, Xiaohong.,Cheng, Gang.,...&Liu, Dongge.(2015).Ventana immunohistochemistry ALK (D5F3) detection of ALK expression in pleural effusion samples of lung adenocarcinoma.PERSONALIZED MEDICINE,12(4),349-357.
MLA Wang, Zheng,et al."Ventana immunohistochemistry ALK (D5F3) detection of ALK expression in pleural effusion samples of lung adenocarcinoma".PERSONALIZED MEDICINE 12.4(2015):349-357.
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