学科主题基础医学
New features of DNA damage by acid hydrolysis in MALDI-TOF mass spectrum
Liu, Dan1; Zou, Xiajuan1; Zhong, Lijun1; Lou, Yaxin1; Yang, Bin1; Yin, Yuxin2
关键词DNA MALDI mass spectrometry Acid hydrolysis Nucleotide damage Terminal site
刊名INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
2014-12-01
DOI10.1016/j.ijms.2014.10.001
374页:20-25
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Physics, Atomic, Molecular & Chemical ; Spectroscopy
研究领域[WOS]Physics ; Spectroscopy
关键词[WOS]NONCOVALENT INTERACTION ; SPECTROMETRY
英文摘要

Acid hydrolysis of DNA oligonucleotides leads to nucleotides damage, especially affecting those containing purine bases. Previous research focused mainly on the types of cleaved nucleobases and the cleavage efficiency. There has been no report about the nucleotide site. In this study, 10 DNA oligonucleotides with differing dAMP and dGMP sites were synthesized to study the influence of these sites with MALDI-TOF MS, which offers a powerful tool with advantages of rapid speed, high sensitivity, high tolerance and simple mass spectra. Results show that, with acid hydrolysis, cleavage of terminal nucleotides differs from those in the middle in the MALDI-TOF mass spectra. In addition, in the course of further degradation, there are obvious differences between nucleotides at the 5′ and 3′-termini. (C) 2014 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000347130300004
项目编号2010CB912202 ; 30930021
资助机构China National Major Scientific Program (973 Project) ; National Natural Science Foundation of China
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64049
专题北京大学基础医学院_北京大学系统生物医学研究所
北京大学医学信息学中心
北京大学医学部管理机构_医学部
北京大学基础医学院
北京大学医药卫生分析中心_研发办公室
作者单位1.Peking Univ, Med & Hlth Analyt Ctr, Prote Lab, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Inst Syst Biomed, Hlth Sci Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Liu, Dan,Zou, Xiajuan,Zhong, Lijun,et al. New features of DNA damage by acid hydrolysis in MALDI-TOF mass spectrum[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2014,374:20-25.
APA Liu, Dan,Zou, Xiajuan,Zhong, Lijun,Lou, Yaxin,Yang, Bin,&Yin, Yuxin.(2014).New features of DNA damage by acid hydrolysis in MALDI-TOF mass spectrum.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,374,20-25.
MLA Liu, Dan,et al."New features of DNA damage by acid hydrolysis in MALDI-TOF mass spectrum".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 374(2014):20-25.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
1-s2.0-S138738061400(475KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Dan]的文章
[Zou, Xiajuan]的文章
[Zhong, Lijun]的文章
百度学术
百度学术中相似的文章
[Liu, Dan]的文章
[Zou, Xiajuan]的文章
[Zhong, Lijun]的文章
必应学术
必应学术中相似的文章
[Liu, Dan]的文章
[Zou, Xiajuan]的文章
[Zhong, Lijun]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1-s2.0-S1387380614004072-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。