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学科主题: 基础医学
题名:
New features of DNA damage by acid hydrolysis in MALDI-TOF mass spectrum
作者: Liu, Dan1; Zou, Xiajuan1; Zhong, Lijun1; Lou, Yaxin1; Yang, Bin1; Yin, Yuxin2
关键词: DNA ; MALDI mass spectrometry ; Acid hydrolysis ; Nucleotide damage ; Terminal site
刊名: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
发表日期: 2014-12-01
DOI: 10.1016/j.ijms.2014.10.001
卷: 374, 页:20-25
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Physics, Atomic, Molecular & Chemical ; Spectroscopy
研究领域[WOS]: Physics ; Spectroscopy
关键词[WOS]: NONCOVALENT INTERACTION ; SPECTROMETRY
英文摘要:

Acid hydrolysis of DNA oligonucleotides leads to nucleotides damage, especially affecting those containing purine bases. Previous research focused mainly on the types of cleaved nucleobases and the cleavage efficiency. There has been no report about the nucleotide site. In this study, 10 DNA oligonucleotides with differing dAMP and dGMP sites were synthesized to study the influence of these sites with MALDI-TOF MS, which offers a powerful tool with advantages of rapid speed, high sensitivity, high tolerance and simple mass spectra. Results show that, with acid hydrolysis, cleavage of terminal nucleotides differs from those in the middle in the MALDI-TOF mass spectra. In addition, in the course of further degradation, there are obvious differences between nucleotides at the 5′ and 3′-termini. (C) 2014 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2010CB912202 ; 30930021
项目资助者: China National Major Scientific Program (973 Project) ; National Natural Science Foundation of China
WOS记录号: WOS:000347130300004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64049
Appears in Collections:基础医学院_北京大学系统生物医学研究所_期刊论文

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作者单位: 1.Peking Univ, Med & Hlth Analyt Ctr, Prote Lab, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Inst Syst Biomed, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Liu, Dan,Zou, Xiajuan,Zhong, Lijun,et al. New features of DNA damage by acid hydrolysis in MALDI-TOF mass spectrum[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2014,374:20-25.
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