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学科主题: 公共卫生
题名:
Reaction mechanism of dicofol removal by cellulase
作者: Wang, Ziyuan1; Yang, Ting1; Zhai, Zihan1; Zhang, Boya2; Zhang, Jianbo1
关键词: Dicofol ; Cellulase ; Endo-glucanases activity ; Oxidative degradation ; OH free radical
刊名: JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
发表日期: 2015-10-01
DOI: 10.1016/j.jes.2015.03.015
卷: 36, 页:22-28
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
关键词[WOS]: BIODEGRADATION KINETICS ; CHILDHOOD-CANCER ; ROT FUNGI ; PESTICIDES ; SOIL ; DDT ; DEGRADATION ; ESTROGEN ; RECEPTOR ; ENZYME
英文摘要:

It remains unclear whether dicofol should be defined as a persistent organic pollutant. Its environmental persistence has gained attention. This study focused on its degradation by cellulase. Cellulase was separated using a gel chromatogram, and its degradation activity towards dicofol involved its endoglucanase activity. By analyzing the kinetic parameters of cellulase reacting with mixed substrates, it was shown that cellulase reacted on dicofol and carboxyl methyl cellulose through two different active centers. Thus, the degradation of dicofol was shown to be an oxidative process by cellulase. Next, by comparing the impacts of tert-butyl alcohol (a typical OH free-radical inhibitor) on the removal efficiencies of dicofol under both cellulase and Fenton reagent systems, it was shown that the removal of dicofol was initiated by OH free radicals produced by cellulase. Finally, 4,4′-dichloro-dibenzophenone and chloride were detected using gas chromatography mass spectrometry and ion chromatography analysis, which supported our hypothesis. The reaction mechanism was analyzed and involved an attack by OH free radicals at the orthocarbon of dicofol, resulting in the degradation product 4,4′-dichloro-dibenzophenone. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.

语种: 英语
所属项目编号: 09Z02ESPCP ; 201209020
项目资助者: special fund provided by the State Key Joint Laboratory of Environmental Simulation and Pollution Control (Peking University) ; Special Research Funding for Public Benefit Industries from National Ministry of Environmental Protection
WOS记录号: WOS:000362983500003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64058
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Peking Univ, Coll Environm Sci & Engn, State Key Joint Lab Environm Simulat & Pollut Con, Collaborat Innovat Ctr Reg Environm Qual, Beijing 100871, Peoples R China
2.Peking Univ, Sch Publ Hlth, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Ziyuan,Yang, Ting,Zhai, Zihan,et al. Reaction mechanism of dicofol removal by cellulase[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2015,36:22-28.
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