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Reaction mechanism of dicofol removal by cellulase
Wang, Ziyuan1; Yang, Ting1; Zhai, Zihan1; Zhang, Boya2; Zhang, Jianbo1
关键词Dicofol Cellulase Endo-glucanases activity Oxidative degradation OH free radical
刊名JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
2015-10-01
DOI10.1016/j.jes.2015.03.015
36页:22-28
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Environmental Sciences
研究领域[WOS]Environmental Sciences & Ecology
关键词[WOS]BIODEGRADATION KINETICS ; CHILDHOOD-CANCER ; ROT FUNGI ; PESTICIDES ; SOIL ; DDT ; DEGRADATION ; ESTROGEN ; RECEPTOR ; ENZYME
英文摘要

It remains unclear whether dicofol should be defined as a persistent organic pollutant. Its environmental persistence has gained attention. This study focused on its degradation by cellulase. Cellulase was separated using a gel chromatogram, and its degradation activity towards dicofol involved its endoglucanase activity. By analyzing the kinetic parameters of cellulase reacting with mixed substrates, it was shown that cellulase reacted on dicofol and carboxyl methyl cellulose through two different active centers. Thus, the degradation of dicofol was shown to be an oxidative process by cellulase. Next, by comparing the impacts of tert-butyl alcohol (a typical OH free-radical inhibitor) on the removal efficiencies of dicofol under both cellulase and Fenton reagent systems, it was shown that the removal of dicofol was initiated by OH free radicals produced by cellulase. Finally, 4,4′-dichloro-dibenzophenone and chloride were detected using gas chromatography mass spectrometry and ion chromatography analysis, which supported our hypothesis. The reaction mechanism was analyzed and involved an attack by OH free radicals at the orthocarbon of dicofol, resulting in the degradation product 4,4′-dichloro-dibenzophenone. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.

语种英语
WOS记录号WOS:000362983500003
项目编号09Z02ESPCP ; 201209020
资助机构special fund provided by the State Key Joint Laboratory of Environmental Simulation and Pollution Control (Peking University) ; Special Research Funding for Public Benefit Industries from National Ministry of Environmental Protection
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64058
专题北京大学公共卫生学院
北京大学第一临床医学院_病理科
北京大学第三临床医学院_呼吸科
作者单位1.Peking Univ, Coll Environm Sci & Engn, State Key Joint Lab Environm Simulat & Pollut Con, Collaborat Innovat Ctr Reg Environm Qual, Beijing 100871, Peoples R China
2.Peking Univ, Sch Publ Hlth, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Wang, Ziyuan,Yang, Ting,Zhai, Zihan,et al. Reaction mechanism of dicofol removal by cellulase[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2015,36:22-28.
APA Wang, Ziyuan,Yang, Ting,Zhai, Zihan,Zhang, Boya,&Zhang, Jianbo.(2015).Reaction mechanism of dicofol removal by cellulase.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,36,22-28.
MLA Wang, Ziyuan,et al."Reaction mechanism of dicofol removal by cellulase".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 36(2015):22-28.
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