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学科主题: 基础医学
题名:
Biorheological changes of dendritic cells at the different differentiation stages
作者: Wang, Xianwei1,2; Chen, Dan1; Zhang, Xiaolan1; Jia, Binbin3; Xie, Lide3; Sun, Dagong1; Ka, Weibo1; Yao, Weijuan1; Wen, Zong-Yao1
关键词: Dendritic cells ; migration ability ; rheological properties
刊名: CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
发表日期: 2010
DOI: 10.3233/CH-2010-1334
卷: 46, 期:4, 页:265-273
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Peripheral Vascular Disease
研究领域[WOS]: Hematology ; Cardiovascular System & Cardiology
关键词[WOS]: CANCER-IMMUNOTHERAPY ; ENDOTHELIAL-CELLS ; MIGRATION ; CYTOSKELETON ; ADHESION ; DEFORMABILITY ; MATURATION ; CD62L ; GENE
英文摘要:

The differentiation, maturation and functioning of Dendritic cells (DCs) are dynamic processes. This study investigated the changes of DCs′ migration ability and biorheological properties during their differentiation. Transmigration assay showed that, DCs′ migration rate was improved significantly as they differentiate (p < 0.05); NSC (Rac1 blocker) treatment could significantly decrease their migration rates (p < 0.05). Confocal images showed that, F-actin uniformly distributed in monocytes; with DC′s differentiation, F-actin began to remodel and gather at the site of dendrites; the images presented surface ruffles and uneven sawtooth-like cytoskeletal structures. Fluorescence polarization analysis showed that, membrane fluidity was increased significantly with DC′s differentiation (p < 0.05). CD62L was upregulated significantly (p < 0.05) on the third and ninth days. CD2 was upregulated significantly (p < 0.05) until the seventh day. DC′s electrophoretic mobility was increased continuously, especially increased significantly from the third day to the fifth day and the final stage (p < 0.05). These results indicate that there are significant changes in the biorheological properties of DCs during their differentiation.

语种: 英语
所属项目编号: 30770532
项目资助者: National Natural Science Foundations of China
WOS记录号: WOS:000285683300002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64063
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Chengde Med Coll, Chengde, Hebei Province, Peoples R China
2.Xinxiang Med Coll, Xinxiang, Henan Province, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Xianwei,Chen, Dan,Zhang, Xiaolan,et al. Biorheological changes of dendritic cells at the different differentiation stages[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2010,46(4):265-273.
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