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学科主题基础医学
Biorheological changes of dendritic cells at the different differentiation stages
Wang, Xianwei1,2; Chen, Dan1; Zhang, Xiaolan1; Jia, Binbin3; Xie, Lide3; Sun, Dagong1; Ka, Weibo1; Yao, Weijuan1; Wen, Zong-Yao1
关键词Dendritic cells migration ability rheological properties
刊名CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
2010
DOI10.3233/CH-2010-1334
46期:4页:265-273
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology ; Peripheral Vascular Disease
研究领域[WOS]Hematology ; Cardiovascular System & Cardiology
关键词[WOS]CANCER-IMMUNOTHERAPY ; ENDOTHELIAL-CELLS ; MIGRATION ; CYTOSKELETON ; ADHESION ; DEFORMABILITY ; MATURATION ; CD62L ; GENE
英文摘要

The differentiation, maturation and functioning of Dendritic cells (DCs) are dynamic processes. This study investigated the changes of DCs′ migration ability and biorheological properties during their differentiation. Transmigration assay showed that, DCs′ migration rate was improved significantly as they differentiate (p < 0.05); NSC (Rac1 blocker) treatment could significantly decrease their migration rates (p < 0.05). Confocal images showed that, F-actin uniformly distributed in monocytes; with DC′s differentiation, F-actin began to remodel and gather at the site of dendrites; the images presented surface ruffles and uneven sawtooth-like cytoskeletal structures. Fluorescence polarization analysis showed that, membrane fluidity was increased significantly with DC′s differentiation (p < 0.05). CD62L was upregulated significantly (p < 0.05) on the third and ninth days. CD2 was upregulated significantly (p < 0.05) until the seventh day. DC′s electrophoretic mobility was increased continuously, especially increased significantly from the third day to the fifth day and the final stage (p < 0.05). These results indicate that there are significant changes in the biorheological properties of DCs during their differentiation.

语种英语
WOS记录号WOS:000285683300002
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64063
专题北京大学基础医学院_生物物理学系
作者单位1.Chengde Med Coll, Chengde, Hebei Province, Peoples R China
2.Xinxiang Med Coll, Xinxiang, Henan Province, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100871, Peoples R China
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Wang, Xianwei,Chen, Dan,Zhang, Xiaolan,et al. Biorheological changes of dendritic cells at the different differentiation stages[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2010,46(4):265-273.
APA Wang, Xianwei.,Chen, Dan.,Zhang, Xiaolan.,Jia, Binbin.,Xie, Lide.,...&Wen, Zong-Yao.(2010).Biorheological changes of dendritic cells at the different differentiation stages.CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,46(4),265-273.
MLA Wang, Xianwei,et al."Biorheological changes of dendritic cells at the different differentiation stages".CLINICAL HEMORHEOLOGY AND MICROCIRCULATION 46.4(2010):265-273.
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