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学科主题: 临床医学
题名:
The effects of co-delivery of BMSC-affinity peptide and rhTGF-beta 1 from coaxial electrospun scaffolds on chondrogenic differentiation
作者: Man, Zhentao1; Yin, Ling2; Shao, Zhenxing1; Zhang, Xin1; Hu, Xiaoqing1; Zhu, Jingxian1; Dai, Linghui1; Huang, Hongjie1; Yuan, Lan4; Zhou, Chunyan3; Chen, Haifeng2; Ao, Yingfang1
关键词: Coaxial electrospun fiber scaffold ; Cartilage tissue engineering ; BMSC-affinity peptide ; rhTGF-beta 1 ; Sustained release
刊名: BIOMATERIALS
发表日期: 2014-06-01
DOI: 10.1016/j.biomaterials.2014.03.031
卷: 35, 期:19, 页:5250-5260
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: MESENCHYMAL STEM-CELLS ; TISSUE ENGINEERING APPLICATIONS ; GROWTH-FACTOR-BETA ; IN-VITRO ; CARTILAGE REGENERATION ; ARTICULAR CHONDROCYTES ; CONTROLLED-RELEASE ; NANOFIBERS ; BONE ; COLLAGEN
英文摘要:

Electrospinning is a promising technology for the fabrication of scaffolds in cartilage tissue engineering. Two other important elements for tissue engineering are seed cells and bioactive factors. Bone marrowderived stem cells (BMSCs) and rhTGF-beta 1 are extensively studied for cartilage regeneration. However, little is known about scaffolds that can both specifically enrich BMSCs and release rhTGF-beta 1 to promote chondrogenic differentiation of the incorporated BMSCs. In this study, we first fabricated coaxial electrospun fibers using a polyvinyl pyrrolidone/bovine serum albumin/rhTGF-beta 1 composite solution as the core fluid and poly(e-caprolactone) solution as the sheath fluid. Structural analysis revealed that scaffold fibers were relatively uniform with a diameter of 674.4 +/- 159.6 nm; the core shell structure of coaxial fibers was homogeneous and proteins were evenly distributed in the core. Subsequently, the BMSCspecific affinity peptide E7 was conjugated to the coaxial electrospun fibers to develop a co-delivery system of rhTGF-beta 1 and E7. The results of H-1 nuclear magnetic resonance indicate that the conjugation between the E7 and scaffolds was covalent. The rhTGF-beta 1 incorporated in E7-modified scaffolds could maintain sustained release and bioactivity. Cell adhesion, spreading, and DNA content analyses indicate that the E7 promoted BMSC initial adhesion, and that the scaffolds containing both E7 and rhTGF-beta 1 (CBrhTE) were the most favorable for BMSC survival. Meanwhile, CBrhTE scaffolds could promote the chondrogenic differentiation ability of BMSCs. Overall, the CBrhTE scaffold could synchronously improve all three of the basic components required for cartilage tissue engineering in vitro, which paves the road for designing and building more efficient tissue scaffolds for cartilage repair. (C) 2014 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2012CB933903
项目资助者: National Basic Research Program of China
WOS记录号: WOS:000335705600021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64073
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
4.Peking Univ, Med & Healthy Anal Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Man, Zhentao,Yin, Ling,Shao, Zhenxing,et al. The effects of co-delivery of BMSC-affinity peptide and rhTGF-beta 1 from coaxial electrospun scaffolds on chondrogenic differentiation[J]. BIOMATERIALS,2014,35(19):5250-5260.
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