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学科主题: 临床医学
题名:
AICAR stimulates IL-6 production via p38 MAPK in cardiac fibroblasts in adult mice: A possible role for AMPK
作者: Du, JH; Xu, N; Song, Y; Xu, M; Lu, ZZ; Han, CD; Zhang, YY
关键词: AMPK ; IL-6 ; p38 MAPK ; cardiac fibroblasts
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2005-12-02
DOI: 10.1016/j.bbrc.2005.09.174
卷: 337, 期:4, 页:1139-1144
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ACTIVATED PROTEIN-KINASE ; TUMOR-NECROSIS-FACTOR ; N-TERMINAL KINASE ; ANGIOTENSIN-II ; GLUCOSE-TRANSPORT ; SKELETAL-MUSCLE ; KAPPA-B ; INTERLEUKIN-6 SYNTHESIS ; SIGNALING PATHWAYS ; GENE-EXPRESSION
英文摘要:

Though known as a sensor of energy balance, AMP-activated protein kinase (AMPK) was recently shown to limit damage and apoptotic activity and contribute to the late preconditioning in heart. Interleukin-6 was also reported to involve in anti-apoptosis and cardio-protection in myocardium. Interestingly, both AMPK activity and IL-6 level were increased in response to ischemia, hypertrophy and oxidative stress. To determine whether AMPK activation will promote IL-6 production, cardiac fibroblasts (CFs) from mice were incubated with AMPK activator, 5-aminoimidazole-4-carboxamide-1-4-ribofuranoside (AICAR). The results demonstrated that AICAR time and dose-dependently stimulated IL-6 production by ELISA and immunoflurescence. Pretreatment with p38 nitogen-activated protein kinase (MAPK) inhibitor blocked AICAR-induced IL-6 production; furthermore, AICAR-activated p38 MAPK phosphorylation by Western blot. To confirm that the increase in IL-6 production is ascribed to AMPK activation, we used another known AMPK activator. metformin. It also dose-dependently potentiated IL-6 production in CFs, and this potentiation could be reversed by p38 MAPK inhibitor. In conclusion, AMPK activation promoted IL-6 production in CFs via p38 MAPK-dependent pathway. (c) 2005 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000233120600019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64074
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: Peking Univ, Inst Vasc Med, Third Hosp, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100083, Peoples R China

Recommended Citation:
Du, JH,Xu, N,Song, Y,et al. AICAR stimulates IL-6 production via p38 MAPK in cardiac fibroblasts in adult mice: A possible role for AMPK[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,337(4):1139-1144.
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