北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 神经生物学系  > 期刊论文
学科主题: 基础医学
题名:
Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain
作者: Han, Ying1; Li, Yan1; Xiao, Xing1; Liu, Jia1; Meng, Xiang-Ling1; Liu, Feng-Yu1; Xing, Guo-Gang1; Wan, You1,2,3
关键词: formaldehyde ; TRPV1 ; cancer pain ; mitogen-activated protein kinase ; phosphatidylinositol 3-kinase
刊名: NEUROSCIENCE BULLETIN
发表日期: 2012-04-01
DOI: 10.1007/s12264-012-1211-0
卷: 28, 期:2, 页:165-172
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: PRIMARY SENSORY NEURONS ; DORSAL-ROOT GANGLION ; COMPLETE FREUNDS-ADJUVANT ; VANILLOID RECEPTOR TRPV1 ; HEAT HYPERALGESIA ; PHOSPHATIDYLINOSITOL 3-KINASE ; KINASE ACTIVATION ; SENSITIZATION ; CONTRIBUTES ; EXPRESSION
英文摘要:

Objective Our previous study showed that tumor tissue-derived formaldehyde at low concentrations plays an important role in bone cancer pain through activating transient receptor potential vanilloid subfamily member 1 (TRPV1). The present study further explored whether this tumor tissue-derived endogenous formaldehyde regulates TRPV1 expression in a rat model of bone cancer pain, and if so, what the possible signal pathways are during the development of this type of pain. Methods A rat model of bone cancer pain was established by injecting living MRMT-1 tumor cells into the tibia. The formaldehyde levels were determined by high performance liquid chromatography, and the expression of TRPV1 was examined with Western blot and RT-PCR. In primary cultured dorsal root ganglion (DRG) neurons, the expression of TRPV1 was assessed after treatment with 100 mu mol/L formaldehyde with or without pre-addition of PD98059 [an inhibitor for extracellular signal-regulated kinase], SB203580 (a p38 inhibitor), SP600125 [an inhibitor for c-Jun N-terminal kinase], BIM [a protein kinase C (PKC) inhibitor] or LY294002 [a phosphatidylinositol 3-kinase (PI3K) inhibitor]. Results In the rat model of bone cancer pain, formaldehyde concentration increased in blood plasma, bone marrow and the spinal cord. TRPV1 protein expression was also increased in the DRG. In primary cultured DRG neurons, 100 mu mol/L formaldehyde significantly increased the TRPV1 expression level. Pre-incubation with PD98059, SB203580, SP600125 or LY294002, but not BIM, inhibited the formaldehyde-induced increase of TRPV1 expression. Conclusion Formaldehyde at a very low concentration up-regulates TRPV1 expression through mitogen-activated protein kinase and PI3K, but not PKC, signaling pathways. These results further support our previous finding that TRPV1 in peripheral afferents plays a role in bone cancer pain.

语种: 英语
所属项目编号: 81070893 ; 81171042 ; 31171063 ; BMU20100014 ; 20090450266
项目资助者: National Natural Science Foundation of China ; Ministry of Education of China ; Ministry of Education ; China Postdoctoral Science Foundation ; Beijing Municipal Commission of Education
WOS记录号: WOS:000304463800009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64083
Appears in Collections:基础医学院_神经生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain.pdf(1024KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Key Lab Neurosci, Minist Educ, Minist Hlth, Beijing 100191, Peoples R China

Recommended Citation:
Han, Ying,Li, Yan,Xiao, Xing,et al. Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain[J]. NEUROSCIENCE BULLETIN,2012,28(2):165-172.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Han, Ying]'s Articles
[Li, Yan]'s Articles
[Xiao, Xing]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Han, Ying]‘s Articles
[Li, Yan]‘s Articles
[Xiao, Xing]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace