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学科主题基础医学
Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain
Han, Ying1; Li, Yan1; Xiao, Xing1; Liu, Jia1; Meng, Xiang-Ling1; Liu, Feng-Yu1; Xing, Guo-Gang1; Wan, You1,2,3
关键词formaldehyde TRPV1 cancer pain mitogen-activated protein kinase phosphatidylinositol 3-kinase
刊名NEUROSCIENCE BULLETIN
2012-04-01
DOI10.1007/s12264-012-1211-0
28期:2页:165-172
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]PRIMARY SENSORY NEURONS ; DORSAL-ROOT GANGLION ; COMPLETE FREUNDS-ADJUVANT ; VANILLOID RECEPTOR TRPV1 ; HEAT HYPERALGESIA ; PHOSPHATIDYLINOSITOL 3-KINASE ; KINASE ACTIVATION ; SENSITIZATION ; CONTRIBUTES ; EXPRESSION
英文摘要

Objective Our previous study showed that tumor tissue-derived formaldehyde at low concentrations plays an important role in bone cancer pain through activating transient receptor potential vanilloid subfamily member 1 (TRPV1). The present study further explored whether this tumor tissue-derived endogenous formaldehyde regulates TRPV1 expression in a rat model of bone cancer pain, and if so, what the possible signal pathways are during the development of this type of pain. Methods A rat model of bone cancer pain was established by injecting living MRMT-1 tumor cells into the tibia. The formaldehyde levels were determined by high performance liquid chromatography, and the expression of TRPV1 was examined with Western blot and RT-PCR. In primary cultured dorsal root ganglion (DRG) neurons, the expression of TRPV1 was assessed after treatment with 100 mu mol/L formaldehyde with or without pre-addition of PD98059 [an inhibitor for extracellular signal-regulated kinase], SB203580 (a p38 inhibitor), SP600125 [an inhibitor for c-Jun N-terminal kinase], BIM [a protein kinase C (PKC) inhibitor] or LY294002 [a phosphatidylinositol 3-kinase (PI3K) inhibitor]. Results In the rat model of bone cancer pain, formaldehyde concentration increased in blood plasma, bone marrow and the spinal cord. TRPV1 protein expression was also increased in the DRG. In primary cultured DRG neurons, 100 mu mol/L formaldehyde significantly increased the TRPV1 expression level. Pre-incubation with PD98059, SB203580, SP600125 or LY294002, but not BIM, inhibited the formaldehyde-induced increase of TRPV1 expression. Conclusion Formaldehyde at a very low concentration up-regulates TRPV1 expression through mitogen-activated protein kinase and PI3K, but not PKC, signaling pathways. These results further support our previous finding that TRPV1 in peripheral afferents plays a role in bone cancer pain.

语种英语
WOS记录号WOS:000304463800009
项目编号81070893 ; 81171042 ; 31171063 ; BMU20100014 ; 20090450266
资助机构National Natural Science Foundation of China ; Ministry of Education of China ; Ministry of Education ; China Postdoctoral Science Foundation ; Beijing Municipal Commission of Education
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64083
专题北京大学基础医学院_神经生物学系
北京大学基础医学院
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Key Lab Neurosci, Minist Educ, Minist Hlth, Beijing 100191, Peoples R China
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Han, Ying,Li, Yan,Xiao, Xing,et al. Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain[J]. NEUROSCIENCE BULLETIN,2012,28(2):165-172.
APA Han, Ying.,Li, Yan.,Xiao, Xing.,Liu, Jia.,Meng, Xiang-Ling.,...&Wan, You.(2012).Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain.NEUROSCIENCE BULLETIN,28(2),165-172.
MLA Han, Ying,et al."Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain".NEUROSCIENCE BULLETIN 28.2(2012):165-172.
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